Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline
ObjectiveOral submucous fibrosis (OSF) is a chronic oral mucosal disease, which exerts a profound impact on patients’ daily life and currently lacks efficacious therapeutic interventions. Epigallocatechin-3-gallate (EGCG), the abundant polyphenol found in green tea, exhibits remarkable anti-fibrotic...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1540559/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832576343452155904 |
|---|---|
| author | Ge Gao Ge Gao Caipeng Lin Caipeng Lin Ruibo Li Xi Xie Hai-Bin Luo Hai-Bin Luo |
| author_facet | Ge Gao Ge Gao Caipeng Lin Caipeng Lin Ruibo Li Xi Xie Hai-Bin Luo Hai-Bin Luo |
| author_sort | Ge Gao |
| collection | DOAJ |
| description | ObjectiveOral submucous fibrosis (OSF) is a chronic oral mucosal disease, which exerts a profound impact on patients’ daily life and currently lacks efficacious therapeutic interventions. Epigallocatechin-3-gallate (EGCG), the abundant polyphenol found in green tea, exhibits remarkable anti-fibrotic effects on the skin. However, the research on OSF regarding EGCG is relatively limited.PurposeWe aimed to investigate the potential therapeutic effect of EGCG against OSF using an arecoline (ARE) -induced rat model and primary rat oral fibroblasts.MethodsPrimary rat oral mucosal fibroblasts (ROMF) were isolated and identified. Optimal ARE concentrations were established using the Cell Counting Kit-8. The impact of ARE on extracellular matrix (ECM)-related protein expression was assessed through RT-qPCR and Western blot techniques. Similarly, the effects of EGCG on ARE-induced ECM changes in ROMF were evaluated. The study also established an OSF model in Sprague-Dawley rats, induced by ARE, with pathological changes characterized using HE and Masson’s staining, further assessing the impact of ARE on ECM-related protein expression in rat oral tissues through RT-qPCR and Western blot methods.ResultsEGCG effectively suppressed the ARE-induced ECM components while concurrently improving the OSF pathological process in vitro and in vivo.ConclusionThe results indicate that the natural product EGCG effectively suppressed the increased ECM components induced by ARE and concurrently improved the OSF pathological process, indicating that EGCG could be potentially a novel anti-fibrotic candidate drug for the treatment of OSF. |
| format | Article |
| id | doaj-art-6f883cfa5ddf42c880733899a0dbf8f5 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-6f883cfa5ddf42c880733899a0dbf8f52025-01-31T06:40:01ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011610.3389/fphar.2025.15405591540559Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecolineGe Gao0Ge Gao1Caipeng Lin2Caipeng Lin3Ruibo Li4Xi Xie5Hai-Bin Luo6Hai-Bin Luo7Key Laboratory of Tropical Biological Resources of Ministry of Education and Hainan Engineering Research Center for Drug Screening and Evaluation, School of Pharmaceutical Sciences, Hainan University, Haikou, ChinaSchool of Life and Health Sciences, Hainan University, Haikou, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education and Hainan Engineering Research Center for Drug Screening and Evaluation, School of Pharmaceutical Sciences, Hainan University, Haikou, ChinaSchool of Life and Health Sciences, Hainan University, Haikou, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education and Hainan Engineering Research Center for Drug Screening and Evaluation, School of Pharmaceutical Sciences, Hainan University, Haikou, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education and Hainan Engineering Research Center for Drug Screening and Evaluation, School of Pharmaceutical Sciences, Hainan University, Haikou, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education and Hainan Engineering Research Center for Drug Screening and Evaluation, School of Pharmaceutical Sciences, Hainan University, Haikou, ChinaSong Li’s Academician Workstation of Hainan University (School of Pharmaceutical Sciences), Sanya, ChinaObjectiveOral submucous fibrosis (OSF) is a chronic oral mucosal disease, which exerts a profound impact on patients’ daily life and currently lacks efficacious therapeutic interventions. Epigallocatechin-3-gallate (EGCG), the abundant polyphenol found in green tea, exhibits remarkable anti-fibrotic effects on the skin. However, the research on OSF regarding EGCG is relatively limited.PurposeWe aimed to investigate the potential therapeutic effect of EGCG against OSF using an arecoline (ARE) -induced rat model and primary rat oral fibroblasts.MethodsPrimary rat oral mucosal fibroblasts (ROMF) were isolated and identified. Optimal ARE concentrations were established using the Cell Counting Kit-8. The impact of ARE on extracellular matrix (ECM)-related protein expression was assessed through RT-qPCR and Western blot techniques. Similarly, the effects of EGCG on ARE-induced ECM changes in ROMF were evaluated. The study also established an OSF model in Sprague-Dawley rats, induced by ARE, with pathological changes characterized using HE and Masson’s staining, further assessing the impact of ARE on ECM-related protein expression in rat oral tissues through RT-qPCR and Western blot methods.ResultsEGCG effectively suppressed the ARE-induced ECM components while concurrently improving the OSF pathological process in vitro and in vivo.ConclusionThe results indicate that the natural product EGCG effectively suppressed the increased ECM components induced by ARE and concurrently improved the OSF pathological process, indicating that EGCG could be potentially a novel anti-fibrotic candidate drug for the treatment of OSF.https://www.frontiersin.org/articles/10.3389/fphar.2025.1540559/fullepigallocatechin-3-gallategreen teaoral submucous fibrosisarecolineextracellular matrix |
| spellingShingle | Ge Gao Ge Gao Caipeng Lin Caipeng Lin Ruibo Li Xi Xie Hai-Bin Luo Hai-Bin Luo Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline Frontiers in Pharmacology epigallocatechin-3-gallate green tea oral submucous fibrosis arecoline extracellular matrix |
| title | Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline |
| title_full | Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline |
| title_fullStr | Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline |
| title_full_unstemmed | Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline |
| title_short | Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline |
| title_sort | epigallocatechin 3 gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline |
| topic | epigallocatechin-3-gallate green tea oral submucous fibrosis arecoline extracellular matrix |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1540559/full |
| work_keys_str_mv | AT gegao epigallocatechin3gallateinhibitsthecollagenaccumulationoforalsubmucousfibrosisinducedbyarecoline AT gegao epigallocatechin3gallateinhibitsthecollagenaccumulationoforalsubmucousfibrosisinducedbyarecoline AT caipenglin epigallocatechin3gallateinhibitsthecollagenaccumulationoforalsubmucousfibrosisinducedbyarecoline AT caipenglin epigallocatechin3gallateinhibitsthecollagenaccumulationoforalsubmucousfibrosisinducedbyarecoline AT ruiboli epigallocatechin3gallateinhibitsthecollagenaccumulationoforalsubmucousfibrosisinducedbyarecoline AT xixie epigallocatechin3gallateinhibitsthecollagenaccumulationoforalsubmucousfibrosisinducedbyarecoline AT haibinluo epigallocatechin3gallateinhibitsthecollagenaccumulationoforalsubmucousfibrosisinducedbyarecoline AT haibinluo epigallocatechin3gallateinhibitsthecollagenaccumulationoforalsubmucousfibrosisinducedbyarecoline |