Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of European part of Russia
Introduction. Bats are recognized as primary natural reservoirs for alpha- and betacoronaviruses. The interspecies transmission of bat coronaviruses to other mammalian hosts, including livestock and humans, can lead to epidemics, epizootics, and global pandemics. Objective. This study aims to des...
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Central Research Institute for Epidemiology
2024-12-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/16702/947 |
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| author | Sergey V. Lenshin Tatyana V. Vishnevskaya Alexey V. Romashin Yulia I. Bulycheva Oleg I. Vyshemirsky Sophya A. Solovyeva Asya K. Gitelman Alexey S. Pazilin Dmitry K. Lvov Ben Hu Zheng-Li Shi Sergey V. Alkhovsky |
| author_facet | Sergey V. Lenshin Tatyana V. Vishnevskaya Alexey V. Romashin Yulia I. Bulycheva Oleg I. Vyshemirsky Sophya A. Solovyeva Asya K. Gitelman Alexey S. Pazilin Dmitry K. Lvov Ben Hu Zheng-Li Shi Sergey V. Alkhovsky |
| author_sort | Sergey V. Lenshin |
| collection | DOAJ |
| description | Introduction. Bats are recognized as primary natural reservoirs for alpha- and betacoronaviruses. The interspecies transmission of bat coronaviruses to other mammalian hosts, including livestock and humans, can lead to epidemics, epizootics, and global pandemics.
Objective. This study aims to describe coronaviruses associated with horseshoe bats (Rhinolophus spp.) in the southern regions of the European part of Russia.
Materials and methods. Fecal samples were collected from bats inhabiting caves on the southern macroslope of the Greater Caucasus (Sochi-Adler region) during 2020, 2021, and 2024. Viral genomes were detected and analyzed using high-throughput sequencing (NGS) and RT-PCR.
Results. A novel alphacoronavirus, designated Kudep virus (GenBank acc. # PQ649435), was identified in R. ferrumequinum. Presumably the Kudep virus represents a novel species within the subgenus Decacovirus of the genus Alphacoronavirus. The virus Showed 72% nucleotide identity to a Cardioderma bat coronavirus from Kenya and up to 67% nucleotide identity to the YN2012 virus group found in horseshoe bats in China. RT-PCR screening revealed active circulation of both Kudep virus and the previously described SARS-like betacoronavirus Khosta-1 in the study area. Infection rates in a single R. ferrumequinum colony during autumn 2021 reached 59.2% and 70.5% for Kudep and Khosta-1, respectively. Frequent co-infections with both viruses were observed in individual bats.
Conclusion. Our findings expand the understanding of the distribution of bat alphacoronaviruses and their genetic diversity. We demonstrate the presence of a persistent natural foci of two potentially zoonotic bat coronaviruses, ecologically associated with R. ferrumequinum in the southern European part of Russia. |
| format | Article |
| id | doaj-art-6f63564edd0e44d48b2aee8328dfaa1e |
| institution | Kabale University |
| issn | 0507-4088 2411-2097 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj-art-6f63564edd0e44d48b2aee8328dfaa1e2025-01-22T15:18:43ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972024-12-0169654655710.36233/0507-4088-2791256Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of European part of RussiaSergey V. Lenshin0https://orcid.org/0000-0001-6815-2869Tatyana V. Vishnevskaya1https://orcid.org/0000-0002-6963-8681Alexey V. Romashin2https://orcid.org/0000-0003-4751-1484Yulia I. Bulycheva3https://orcid.org/0000-0002-6798-7925Oleg I. Vyshemirsky4https://orcid.org/0000-0002-5345-8926Sophya A. Solovyeva5https://orcid.org/0009-0006-7021-7143Asya K. Gitelman6https://orcid.org/0000-0002-8410-2332Alexey S. Pazilin7https://orcid.org/0009-0002-5665-409XDmitry K. Lvov8https://orcid.org/0000-0001-8176-6582Ben Hu9https://orcid.org/0000-0001-9194-3474Zheng-Li Shi10https://orcid.org/0000-0001-8089-163XSergey V. Alkhovsky11https://orcid.org/0000-0001-6913-5841Stavropol Plague Control Research Institute of RospotrebnadzorD.I. Ivanovsky institute of virology of N.F Gamaleya national research center on epidemiology and microbiology of Ministry of health of Russian FederationSochi National ParkD.I. Ivanovsky institute of virology of N.F Gamaleya national research center on epidemiology and microbiology of Ministry of health of Russian FederationNational Research Center «Kurchatov Institute»D.I. Ivanovsky institute of virology of N.F Gamaleya national research center on epidemiology and microbiology of Ministry of health of Russian FederationD.I. Ivanovsky institute of virology of N.F Gamaleya national research center on epidemiology and microbiology of Ministry of health of Russian FederationD.I. Ivanovsky institute of virology of N.F Gamaleya national research center on epidemiology and microbiology of Ministry of health of Russian FederationD.I. Ivanovsky institute of virology of N.F Gamaleya national research center on epidemiology and microbiology of Ministry of health of Russian FederationWuhan Institute of Virology, Chinese Academy of SciencesWuhan Institute of Virology, Chinese Academy of SciencesD.I. Ivanovsky institute of virology of N.F Gamaleya national research center on epidemiology and microbiology of Ministry of health of Russian FederationIntroduction. Bats are recognized as primary natural reservoirs for alpha- and betacoronaviruses. The interspecies transmission of bat coronaviruses to other mammalian hosts, including livestock and humans, can lead to epidemics, epizootics, and global pandemics. Objective. This study aims to describe coronaviruses associated with horseshoe bats (Rhinolophus spp.) in the southern regions of the European part of Russia. Materials and methods. Fecal samples were collected from bats inhabiting caves on the southern macroslope of the Greater Caucasus (Sochi-Adler region) during 2020, 2021, and 2024. Viral genomes were detected and analyzed using high-throughput sequencing (NGS) and RT-PCR. Results. A novel alphacoronavirus, designated Kudep virus (GenBank acc. # PQ649435), was identified in R. ferrumequinum. Presumably the Kudep virus represents a novel species within the subgenus Decacovirus of the genus Alphacoronavirus. The virus Showed 72% nucleotide identity to a Cardioderma bat coronavirus from Kenya and up to 67% nucleotide identity to the YN2012 virus group found in horseshoe bats in China. RT-PCR screening revealed active circulation of both Kudep virus and the previously described SARS-like betacoronavirus Khosta-1 in the study area. Infection rates in a single R. ferrumequinum colony during autumn 2021 reached 59.2% and 70.5% for Kudep and Khosta-1, respectively. Frequent co-infections with both viruses were observed in individual bats. Conclusion. Our findings expand the understanding of the distribution of bat alphacoronaviruses and their genetic diversity. We demonstrate the presence of a persistent natural foci of two potentially zoonotic bat coronaviruses, ecologically associated with R. ferrumequinum in the southern European part of Russia.https://virusjour.crie.ru/jour/article/viewFile/16702/947batsbat coronaviruseskudep viruskhosta-1 virusemerging and re-emerging infectionsalphacoronavirusessars-cov-2horseshoe bats |
| spellingShingle | Sergey V. Lenshin Tatyana V. Vishnevskaya Alexey V. Romashin Yulia I. Bulycheva Oleg I. Vyshemirsky Sophya A. Solovyeva Asya K. Gitelman Alexey S. Pazilin Dmitry K. Lvov Ben Hu Zheng-Li Shi Sergey V. Alkhovsky Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of European part of Russia Вопросы вирусологии bats bat coronaviruses kudep virus khosta-1 virus emerging and re-emerging infections alphacoronaviruses sars-cov-2 horseshoe bats |
| title | Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of European part of Russia |
| title_full | Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of European part of Russia |
| title_fullStr | Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of European part of Russia |
| title_full_unstemmed | Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of European part of Russia |
| title_short | Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of European part of Russia |
| title_sort | identification of a new alphacoronavirus coronaviridae i alphacoronavirus i associated with the greater horseshoe bat i rhinolophus ferrumequinum i in the south of european part of russia |
| topic | bats bat coronaviruses kudep virus khosta-1 virus emerging and re-emerging infections alphacoronaviruses sars-cov-2 horseshoe bats |
| url | https://virusjour.crie.ru/jour/article/viewFile/16702/947 |
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