Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B
We are engaged in airborne transmission and epidemiology studies of respiratory pathogens, with particular interest in human parainfluenza virus type 4 (hPIV-4) and other lesser studied viruses. In this paper, hPIV-4 was detected in primary rhesus monkey kidney (PRMK) cells that had been inoculated...
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| Language: | English |
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Wiley
2012-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.6064/2012/871201 |
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| author | John A. Lednicky Thomas B. Waltzek Micah D. Halpern Sara B. Hamilton |
| author_facet | John A. Lednicky Thomas B. Waltzek Micah D. Halpern Sara B. Hamilton |
| author_sort | John A. Lednicky |
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| description | We are engaged in airborne transmission and epidemiology studies of respiratory pathogens, with particular interest in human parainfluenza virus type 4 (hPIV-4) and other lesser studied viruses. In this paper, hPIV-4 was detected in primary rhesus monkey kidney (PRMK) cells that had been inoculated with nasopharyngeal swab material obtained from a child with a mild upper respiratory tract illness. Attempts to isolate the virus in pure culture were hampered by the presence of a fast-growing simian spumavirus that was a contaminant of the PRMK cells. Total RNA was extracted from the PRMK cell culture, and PCR followed by sequencing of a subgenomic section of the fusion protein gene suggested the hPIV-4 was subtype 4B. At the time of this work, two complete but dissimilar hPIV-4B genomes had been deposited by others in GenBank. To gain better insights on hPIV-4B, and to test methods that we are developing for viral forensics, the entire genomic sequence of our virus was determined from archived RNA. The hPIV-4B genomic sequence that we determined conforms to the paramyxovirus “rule of six.” Here, we compare and contrast the genetic features of the three completely sequenced hPIV-4B genomes currently present in GenBank. |
| format | Article |
| id | doaj-art-6f5b24d7bc784a8cac911c7cf29b770a |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
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| series | Scientifica |
| spelling | doaj-art-6f5b24d7bc784a8cac911c7cf29b770a2025-02-03T06:11:35ZengWileyScientifica2090-908X2012-01-01201210.6064/2012/871201871201Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4BJohn A. Lednicky0Thomas B. Waltzek1Micah D. Halpern2Sara B. Hamilton3Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, P.O. Box 100188, Gainesville, FL 32610-0188, USADepartment of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, P.O. Box 100188, Gainesville, FL 32610-0188, USAGenSol Diagnostics, 4945 Apollo Avenue, St. Cloud, FL 34773, USAMedical Countermeasures Division, MRIGlobal, 425 Volker Boulevard, Kansas City, MO 64110, USAWe are engaged in airborne transmission and epidemiology studies of respiratory pathogens, with particular interest in human parainfluenza virus type 4 (hPIV-4) and other lesser studied viruses. In this paper, hPIV-4 was detected in primary rhesus monkey kidney (PRMK) cells that had been inoculated with nasopharyngeal swab material obtained from a child with a mild upper respiratory tract illness. Attempts to isolate the virus in pure culture were hampered by the presence of a fast-growing simian spumavirus that was a contaminant of the PRMK cells. Total RNA was extracted from the PRMK cell culture, and PCR followed by sequencing of a subgenomic section of the fusion protein gene suggested the hPIV-4 was subtype 4B. At the time of this work, two complete but dissimilar hPIV-4B genomes had been deposited by others in GenBank. To gain better insights on hPIV-4B, and to test methods that we are developing for viral forensics, the entire genomic sequence of our virus was determined from archived RNA. The hPIV-4B genomic sequence that we determined conforms to the paramyxovirus “rule of six.” Here, we compare and contrast the genetic features of the three completely sequenced hPIV-4B genomes currently present in GenBank.http://dx.doi.org/10.6064/2012/871201 |
| spellingShingle | John A. Lednicky Thomas B. Waltzek Micah D. Halpern Sara B. Hamilton Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B Scientifica |
| title | Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B |
| title_full | Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B |
| title_fullStr | Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B |
| title_full_unstemmed | Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B |
| title_short | Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B |
| title_sort | comparative analysis of the full length genome sequence of a clinical isolate of human parainfluenza virus 4b |
| url | http://dx.doi.org/10.6064/2012/871201 |
| work_keys_str_mv | AT johnalednicky comparativeanalysisofthefulllengthgenomesequenceofaclinicalisolateofhumanparainfluenzavirus4b AT thomasbwaltzek comparativeanalysisofthefulllengthgenomesequenceofaclinicalisolateofhumanparainfluenzavirus4b AT micahdhalpern comparativeanalysisofthefulllengthgenomesequenceofaclinicalisolateofhumanparainfluenzavirus4b AT sarabhamilton comparativeanalysisofthefulllengthgenomesequenceofaclinicalisolateofhumanparainfluenzavirus4b |