Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays
Drug-induced nephrotoxicity causes huge morbidity and mortality at massive financial cost. The greatest burden of drug-induced acute kidney injury falls on the proximal tubular cells. To maintain their structure and function, renal proximal tubular cells need the shear stress from tubular fluid flow...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2021/6643324 |
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| author | Holly H. Birdsall Timothy G. Hammond |
| author_facet | Holly H. Birdsall Timothy G. Hammond |
| author_sort | Holly H. Birdsall |
| collection | DOAJ |
| description | Drug-induced nephrotoxicity causes huge morbidity and mortality at massive financial cost. The greatest burden of drug-induced acute kidney injury falls on the proximal tubular cells. To maintain their structure and function, renal proximal tubular cells need the shear stress from tubular fluid flow. Diverse techniques to reintroduce shear stress have been studied in a variety of proximal tubular like cell culture models. These studies often have limited replicates because of the huge cost of equipment and do not report all relevant parameters to allow reproduction and comparison of studies between labs. This review codifies the techniques used to reintroduce shear stress, the cell lines utilized, and the biological outcomes reported. Further, we propose a set of interventions to enhance future cell biology understanding of nephrotoxicity using cell culture models. |
| format | Article |
| id | doaj-art-6f3c64c94bb64699b44ef724b622ff6e |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-6f3c64c94bb64699b44ef724b622ff6e2025-02-03T06:43:49ZengWileyJournal of Toxicology1687-81911687-82052021-01-01202110.1155/2021/66433246643324Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity AssaysHolly H. Birdsall0Timothy G. Hammond1Departments of Otorhinolaryngology, Immunology, and Psychiatry, Baylor College of Medicine, Houston, TX 77030, USANephrology Section, Department of Medicine, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans 70112, USADrug-induced nephrotoxicity causes huge morbidity and mortality at massive financial cost. The greatest burden of drug-induced acute kidney injury falls on the proximal tubular cells. To maintain their structure and function, renal proximal tubular cells need the shear stress from tubular fluid flow. Diverse techniques to reintroduce shear stress have been studied in a variety of proximal tubular like cell culture models. These studies often have limited replicates because of the huge cost of equipment and do not report all relevant parameters to allow reproduction and comparison of studies between labs. This review codifies the techniques used to reintroduce shear stress, the cell lines utilized, and the biological outcomes reported. Further, we propose a set of interventions to enhance future cell biology understanding of nephrotoxicity using cell culture models.http://dx.doi.org/10.1155/2021/6643324 |
| spellingShingle | Holly H. Birdsall Timothy G. Hammond Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays Journal of Toxicology |
| title | Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays |
| title_full | Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays |
| title_fullStr | Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays |
| title_full_unstemmed | Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays |
| title_short | Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays |
| title_sort | role of shear stress on renal proximal tubular cells for nephrotoxicity assays |
| url | http://dx.doi.org/10.1155/2021/6643324 |
| work_keys_str_mv | AT hollyhbirdsall roleofshearstressonrenalproximaltubularcellsfornephrotoxicityassays AT timothyghammond roleofshearstressonrenalproximaltubularcellsfornephrotoxicityassays |