Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010

Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purp...

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Main Authors: Arindam Sarkar, Debjani Taraphdar, Shyamalendu Chatterjee
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Journal of Tropical Medicine
Online Access:http://dx.doi.org/10.1155/2012/960329
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author Arindam Sarkar
Debjani Taraphdar
Shyamalendu Chatterjee
author_facet Arindam Sarkar
Debjani Taraphdar
Shyamalendu Chatterjee
author_sort Arindam Sarkar
collection DOAJ
description Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by RT-PCR test for the detection of serotypes. Only 173 samples were ELISA positive. Out of 378 samples, 108 were RT-PCR positive. Out of 108 samples, 74 samples had monotypic infection with different serotypes of DENV and 33 samples had dual infections with DENV-2 and DENV-3. Only one sample had the infection with DENV-1, DENV-2, and DENV-3. DHF was found mainly among the patients, infected with multiple dengue serotypes. Only 3 dengue monotypic infected patients had suffered from DHF.
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spelling doaj-art-6f3904b7cc1e4194af642c9eac1845d22025-02-03T01:12:17ZengWileyJournal of Tropical Medicine1687-96861687-96942012-01-01201210.1155/2012/960329960329Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010Arindam Sarkar0Debjani Taraphdar1Shyamalendu Chatterjee2ICMR Virus Unit, Kolkata, ID & BG Hospital Campus 57, Dr. S. C. Banerjee Road, Beliaghata, Kolkata 700010, IndiaICMR Virus Unit, Kolkata, ID & BG Hospital Campus 57, Dr. S. C. Banerjee Road, Beliaghata, Kolkata 700010, IndiaICMR Virus Unit, Kolkata, ID & BG Hospital Campus 57, Dr. S. C. Banerjee Road, Beliaghata, Kolkata 700010, IndiaDengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by RT-PCR test for the detection of serotypes. Only 173 samples were ELISA positive. Out of 378 samples, 108 were RT-PCR positive. Out of 108 samples, 74 samples had monotypic infection with different serotypes of DENV and 33 samples had dual infections with DENV-2 and DENV-3. Only one sample had the infection with DENV-1, DENV-2, and DENV-3. DHF was found mainly among the patients, infected with multiple dengue serotypes. Only 3 dengue monotypic infected patients had suffered from DHF.http://dx.doi.org/10.1155/2012/960329
spellingShingle Arindam Sarkar
Debjani Taraphdar
Shyamalendu Chatterjee
Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010
Journal of Tropical Medicine
title Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010
title_full Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010
title_fullStr Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010
title_full_unstemmed Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010
title_short Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010
title_sort molecular typing of dengue virus circulating in kolkata india in 2010
url http://dx.doi.org/10.1155/2012/960329
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