Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics
Radix Dipsaci (RD) is the dry root of the Dipsacus asper Wall. ex DC., which is commonly used for tonifying the kidney and strengthening bone. The purpose of this study was to analyze the difference between raw and salt-processed RD from the chemical composition comprehensively. The fingerprints of...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2024/2109127 |
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| author | Hangsha Wu Yue Lv Rui Tang Mingfang Zhao Yafei Li Feiyang Wei Changyu Li Weihong Ge Weifeng Du |
| author_facet | Hangsha Wu Yue Lv Rui Tang Mingfang Zhao Yafei Li Feiyang Wei Changyu Li Weihong Ge Weifeng Du |
| author_sort | Hangsha Wu |
| collection | DOAJ |
| description | Radix Dipsaci (RD) is the dry root of the Dipsacus asper Wall. ex DC., which is commonly used for tonifying the kidney and strengthening bone. The purpose of this study was to analyze the difference between raw and salt-processed RD from the chemical composition comprehensively. The fingerprints of raw and salt-processed RD were established by HPLC-DAD to determine the contents of loganin (LN), asperosaponin VI (AVI), caffeic acid (CaA), dipsanoside A (DA), dipsanoside B (DB), chlorogenic acid (CA), loganic acid (LA), isochlorogenic acid A (IA), isochlorogenic acid B (IB), and isochlorogenic acid C (IC). The results showed that after processing with salt, the components with increased contents were LA, CaA, DA, and AVI, and the components with decreased contents were CA, LN, IB, IA, IC, and DB. Then, the chemometric methods such as principal component analysis (PCA) and fisher discriminant analysis (FDA) were used to evaluate the quality of raw and salt-processed RD. In the classification of raw and salt-processed RD, the order of importance of each chemical component was LA > DB > IA > IC > IB > LN > CA > DA > AVI > CaA. These integrated methods successfully assessed the quality of raw and salt-processed RD, which will provide guidance for the development of RD as a clinical medication. |
| format | Article |
| id | doaj-art-6f198f6b938a446fb1593d59991c0400 |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-6f198f6b938a446fb1593d59991c04002025-02-03T01:31:59ZengWileyInternational Journal of Analytical Chemistry1687-87792024-01-01202410.1155/2024/2109127Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with ChemometricsHangsha Wu0Yue Lv1Rui Tang2Mingfang Zhao3Yafei Li4Feiyang Wei5Changyu Li6Weihong Ge7Weifeng Du8School of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesAcademy of Chinese Medical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesRadix Dipsaci (RD) is the dry root of the Dipsacus asper Wall. ex DC., which is commonly used for tonifying the kidney and strengthening bone. The purpose of this study was to analyze the difference between raw and salt-processed RD from the chemical composition comprehensively. The fingerprints of raw and salt-processed RD were established by HPLC-DAD to determine the contents of loganin (LN), asperosaponin VI (AVI), caffeic acid (CaA), dipsanoside A (DA), dipsanoside B (DB), chlorogenic acid (CA), loganic acid (LA), isochlorogenic acid A (IA), isochlorogenic acid B (IB), and isochlorogenic acid C (IC). The results showed that after processing with salt, the components with increased contents were LA, CaA, DA, and AVI, and the components with decreased contents were CA, LN, IB, IA, IC, and DB. Then, the chemometric methods such as principal component analysis (PCA) and fisher discriminant analysis (FDA) were used to evaluate the quality of raw and salt-processed RD. In the classification of raw and salt-processed RD, the order of importance of each chemical component was LA > DB > IA > IC > IB > LN > CA > DA > AVI > CaA. These integrated methods successfully assessed the quality of raw and salt-processed RD, which will provide guidance for the development of RD as a clinical medication.http://dx.doi.org/10.1155/2024/2109127 |
| spellingShingle | Hangsha Wu Yue Lv Rui Tang Mingfang Zhao Yafei Li Feiyang Wei Changyu Li Weihong Ge Weifeng Du Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics International Journal of Analytical Chemistry |
| title | Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics |
| title_full | Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics |
| title_fullStr | Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics |
| title_full_unstemmed | Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics |
| title_short | Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics |
| title_sort | analysis of quality differences in radix dipsaci before and after processing with salt based on quantitative control of hplc multi indicator components combined with chemometrics |
| url | http://dx.doi.org/10.1155/2024/2109127 |
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