<i>Epigynum auritum</i>-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial Applications
The use of biomass feedstocks for producing high-value-added chemicals is gaining significant attention in the academic community. In this study, near-infrared carbon dots (NIR-CDs) with antimicrobial and bioimaging functions were prepared from <i>Epigynum auritum</i> branches and leaves...
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MDPI AG
2025-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/2/422 |
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| author | Wenfeng Shi Jiahui Li Junmei Pu Guiguang Cheng Yaping Liu Shanshan Xiao Jianxin Cao |
| author_facet | Wenfeng Shi Jiahui Li Junmei Pu Guiguang Cheng Yaping Liu Shanshan Xiao Jianxin Cao |
| author_sort | Wenfeng Shi |
| collection | DOAJ |
| description | The use of biomass feedstocks for producing high-value-added chemicals is gaining significant attention in the academic community. In this study, near-infrared carbon dots (NIR-CDs) with antimicrobial and bioimaging functions were prepared from <i>Epigynum auritum</i> branches and leaves using a novel green synthesis approach. The spectral properties of the synthesized NIR-CDs were characterized by ultraviolet–visible (UV-Vis) absorption and fluorescence spectroscopy. The crystal structures of the NIR-CDs were further characterized by high-resolution transmission electron microscopy (HR-TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), and X-ray diffraction (XRD). The NIR-CDs exhibited minimal toxicity, excellent biocompatibility, and high penetrability in both in vivo and in vitro environments, making them ideal luminescent probes for bioimaging applications. Moreover, the antimicrobial activity of NIR-CDs was tested against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>), showing significant bacterial growth inhibition. The antimicrobial effect is likely attributed to the NIR-CDs disrupting the cell membrane integrity, leading to the leakage of the intracellular contents. Therefore, NIR-CDs hold promise as fluorescent bioimaging probes and antimicrobial agents. |
| format | Article |
| id | doaj-art-6ee1c07f32b64eebbcc0d53b1f070330 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-6ee1c07f32b64eebbcc0d53b1f0703302025-01-24T13:43:59ZengMDPI AGMolecules1420-30492025-01-0130242210.3390/molecules30020422<i>Epigynum auritum</i>-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial ApplicationsWenfeng Shi0Jiahui Li1Junmei Pu2Guiguang Cheng3Yaping Liu4Shanshan Xiao5Jianxin Cao6Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaThe use of biomass feedstocks for producing high-value-added chemicals is gaining significant attention in the academic community. In this study, near-infrared carbon dots (NIR-CDs) with antimicrobial and bioimaging functions were prepared from <i>Epigynum auritum</i> branches and leaves using a novel green synthesis approach. The spectral properties of the synthesized NIR-CDs were characterized by ultraviolet–visible (UV-Vis) absorption and fluorescence spectroscopy. The crystal structures of the NIR-CDs were further characterized by high-resolution transmission electron microscopy (HR-TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), and X-ray diffraction (XRD). The NIR-CDs exhibited minimal toxicity, excellent biocompatibility, and high penetrability in both in vivo and in vitro environments, making them ideal luminescent probes for bioimaging applications. Moreover, the antimicrobial activity of NIR-CDs was tested against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>), showing significant bacterial growth inhibition. The antimicrobial effect is likely attributed to the NIR-CDs disrupting the cell membrane integrity, leading to the leakage of the intracellular contents. Therefore, NIR-CDs hold promise as fluorescent bioimaging probes and antimicrobial agents.https://www.mdpi.com/1420-3049/30/2/422near-infrared carbon dotsbacteriostaticbioimaging |
| spellingShingle | Wenfeng Shi Jiahui Li Junmei Pu Guiguang Cheng Yaping Liu Shanshan Xiao Jianxin Cao <i>Epigynum auritum</i>-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial Applications Molecules near-infrared carbon dots bacteriostatic bioimaging |
| title | <i>Epigynum auritum</i>-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial Applications |
| title_full | <i>Epigynum auritum</i>-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial Applications |
| title_fullStr | <i>Epigynum auritum</i>-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial Applications |
| title_full_unstemmed | <i>Epigynum auritum</i>-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial Applications |
| title_short | <i>Epigynum auritum</i>-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial Applications |
| title_sort | i epigynum auritum i derived near infrared carbon dots for bioimaging and antimicrobial applications |
| topic | near-infrared carbon dots bacteriostatic bioimaging |
| url | https://www.mdpi.com/1420-3049/30/2/422 |
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