A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in Mice
Mice are the most frequently used animals in pharmacokinetic studies; however, collecting series of blood samples from mice is difficult because of their small sizes and tiny vessels. In addition, due to the small sample size, it is problematic to perform high required quantification. Thus, present...
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| Format: | Article |
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Wiley
2022-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/5952436 |
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| author | Ying Xu Song-xia Zhang Jing Guo Li-jie Chen Yu-ligh Liou Tai Rao Jing-bo Peng Ying Guo Wei-hua Huang Zhi-rong Tan Dong-sheng Ou-yang Hong-hao Zhou Wei Zhang Yao Chen |
| author_facet | Ying Xu Song-xia Zhang Jing Guo Li-jie Chen Yu-ligh Liou Tai Rao Jing-bo Peng Ying Guo Wei-hua Huang Zhi-rong Tan Dong-sheng Ou-yang Hong-hao Zhou Wei Zhang Yao Chen |
| author_sort | Ying Xu |
| collection | DOAJ |
| description | Mice are the most frequently used animals in pharmacokinetic studies; however, collecting series of blood samples from mice is difficult because of their small sizes and tiny vessels. In addition, due to the small sample size, it is problematic to perform high required quantification. Thus, present work aims to find an effective strategy for overcoming these challenges using trans-resveratrol as a tool drug. Based on the idea of a joint technology, the capillary microsampling (CMS) was chosen for blood sample collection from mice after delivery of trans-resveratrol (150 mg/kg) by gavage, and a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of trans-resveratrol and its main metabolites. All the mouse blood samples were exactly collected by CMS without obvious deviation. This provided credible samples for subsequent quantitative analysis. The HPLC-MS/MS method was found to be sensitive, accurate, and repeatable, and the pharmacokinetic parameters for all analytes were comparable with those reported in previous studies. However, the present joint technology offers the advantages of less animal damage, easy for sample preparation, and improved reliability. It has overcome some of the major limitations revealed in previous pharmacokinetic studies in mice and therefore provides a more effective option for future studies. |
| format | Article |
| id | doaj-art-6ec2c148e4344cab83125a7f4e3be213 |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-6ec2c148e4344cab83125a7f4e3be2132025-02-03T01:24:44ZengWileyJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/5952436A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in MiceYing Xu0Song-xia Zhang1Jing Guo2Li-jie Chen3Yu-ligh Liou4Tai Rao5Jing-bo Peng6Ying Guo7Wei-hua Huang8Zhi-rong Tan9Dong-sheng Ou-yang10Hong-hao Zhou11Wei Zhang12Yao Chen13Department of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyMice are the most frequently used animals in pharmacokinetic studies; however, collecting series of blood samples from mice is difficult because of their small sizes and tiny vessels. In addition, due to the small sample size, it is problematic to perform high required quantification. Thus, present work aims to find an effective strategy for overcoming these challenges using trans-resveratrol as a tool drug. Based on the idea of a joint technology, the capillary microsampling (CMS) was chosen for blood sample collection from mice after delivery of trans-resveratrol (150 mg/kg) by gavage, and a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of trans-resveratrol and its main metabolites. All the mouse blood samples were exactly collected by CMS without obvious deviation. This provided credible samples for subsequent quantitative analysis. The HPLC-MS/MS method was found to be sensitive, accurate, and repeatable, and the pharmacokinetic parameters for all analytes were comparable with those reported in previous studies. However, the present joint technology offers the advantages of less animal damage, easy for sample preparation, and improved reliability. It has overcome some of the major limitations revealed in previous pharmacokinetic studies in mice and therefore provides a more effective option for future studies.http://dx.doi.org/10.1155/2022/5952436 |
| spellingShingle | Ying Xu Song-xia Zhang Jing Guo Li-jie Chen Yu-ligh Liou Tai Rao Jing-bo Peng Ying Guo Wei-hua Huang Zhi-rong Tan Dong-sheng Ou-yang Hong-hao Zhou Wei Zhang Yao Chen A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in Mice Journal of Analytical Methods in Chemistry |
| title | A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in Mice |
| title_full | A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in Mice |
| title_fullStr | A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in Mice |
| title_full_unstemmed | A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in Mice |
| title_short | A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in Mice |
| title_sort | joint technology combining the advantages of capillary microsampling with mass spectrometry applied to the trans resveratrol pharmacokinetic study in mice |
| url | http://dx.doi.org/10.1155/2022/5952436 |
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