PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway
Objective. To assess the expression levels of exchange protein 1 directly activated by cAMP (Epac1) and phosphodiesterase 4 (PDE4) in rectal carcinoma, and their associations with clinicopathological indexes. In addition, the associations of PDE4 and Epac1 with A-kinase anchor protein 95, connexin 4...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2019/7145198 |
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| author | Xiangyu Kong Ganghao Ai Dai Wang Renzhen Chen Dongbei Guo Youliang Yao Kai Wang Guiye Liang Fengjie Qi Wenzhi Liu Yongxing Zhang |
| author_facet | Xiangyu Kong Ganghao Ai Dai Wang Renzhen Chen Dongbei Guo Youliang Yao Kai Wang Guiye Liang Fengjie Qi Wenzhi Liu Yongxing Zhang |
| author_sort | Xiangyu Kong |
| collection | DOAJ |
| description | Objective. To assess the expression levels of exchange protein 1 directly activated by cAMP (Epac1) and phosphodiesterase 4 (PDE4) in rectal carcinoma, and their associations with clinicopathological indexes. In addition, the associations of PDE4 and Epac1 with A-kinase anchor protein 95, connexin 43, cyclin D1, and cyclin E1 were evaluated. Methods. The PV-9000 two-step immunohistochemistry method was used to determine protein expression in 44 rectal carcinoma tissue samples and 16 paracarcinoma tissue specimens. Results. The positive rate of PDE4 protein expression in rectal carcinoma tissues was higher than that of paracarcinoma tissues (59.09% vs. 12.5%, P<0.05). Similar findings were obtained for Epac1 (55% vs. 6.25%, P<0.05). No significant associations of PDE4 and Epac1 with degree of differentiation, histological type, and lymph node metastasis were found in rectal carcinoma (P>0.05). Correlations between PDE4 and Epac1, PDE4 and Cx43, PDE4 and cyclin E1, and Epac1 and Cx43 were observed (all P<0.05). There was no correlation between the other protein pairs examined (P>0.05). Conclusion. PDE4 and Epac1 expression levels are increased in rectal carcinoma tissues, suggesting that the two proteins may be involved in the development of this malignancy. Meanwhile, correlations between PDE4 and Epac1, PDE4 and Cx43, PDE4 and cyclin E1, and Epac1 and Cx43 suggested synergistic effects of these proteins in promoting rectal carcinoma. |
| format | Article |
| id | doaj-art-6eb741d7a36f422f99b8021fce260418 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-6eb741d7a36f422f99b8021fce2604182025-02-03T01:00:17ZengWileyAnalytical Cellular Pathology2210-71772210-71852019-01-01201910.1155/2019/71451987145198PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP PathwayXiangyu Kong0Ganghao Ai1Dai Wang2Renzhen Chen3Dongbei Guo4Youliang Yao5Kai Wang6Guiye Liang7Fengjie Qi8Wenzhi Liu9Yongxing Zhang10Department of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001 Liaoning, ChinaDepartment of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001 Liaoning, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, ChinaDepartment of Pathology, The third Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518001, ChinaDepartment of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001 Liaoning, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, ChinaObjective. To assess the expression levels of exchange protein 1 directly activated by cAMP (Epac1) and phosphodiesterase 4 (PDE4) in rectal carcinoma, and their associations with clinicopathological indexes. In addition, the associations of PDE4 and Epac1 with A-kinase anchor protein 95, connexin 43, cyclin D1, and cyclin E1 were evaluated. Methods. The PV-9000 two-step immunohistochemistry method was used to determine protein expression in 44 rectal carcinoma tissue samples and 16 paracarcinoma tissue specimens. Results. The positive rate of PDE4 protein expression in rectal carcinoma tissues was higher than that of paracarcinoma tissues (59.09% vs. 12.5%, P<0.05). Similar findings were obtained for Epac1 (55% vs. 6.25%, P<0.05). No significant associations of PDE4 and Epac1 with degree of differentiation, histological type, and lymph node metastasis were found in rectal carcinoma (P>0.05). Correlations between PDE4 and Epac1, PDE4 and Cx43, PDE4 and cyclin E1, and Epac1 and Cx43 were observed (all P<0.05). There was no correlation between the other protein pairs examined (P>0.05). Conclusion. PDE4 and Epac1 expression levels are increased in rectal carcinoma tissues, suggesting that the two proteins may be involved in the development of this malignancy. Meanwhile, correlations between PDE4 and Epac1, PDE4 and Cx43, PDE4 and cyclin E1, and Epac1 and Cx43 suggested synergistic effects of these proteins in promoting rectal carcinoma.http://dx.doi.org/10.1155/2019/7145198 |
| spellingShingle | Xiangyu Kong Ganghao Ai Dai Wang Renzhen Chen Dongbei Guo Youliang Yao Kai Wang Guiye Liang Fengjie Qi Wenzhi Liu Yongxing Zhang PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway Analytical Cellular Pathology |
| title | PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway |
| title_full | PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway |
| title_fullStr | PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway |
| title_full_unstemmed | PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway |
| title_short | PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway |
| title_sort | pde4 and epac1 synergistically promote rectal carcinoma via the camp pathway |
| url | http://dx.doi.org/10.1155/2019/7145198 |
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