Removal of dsRNA byproducts using affinity chromatography

Double-stranded RNA (dsRNA) molecules are immunogenic byproducts of in vitro transcription of single-stranded RNA (ssRNA). Removal of dsRNA from ssRNA is difficult because the byproducts have similar sizes, sequences, and charges to the desired ssRNA. Here, we describe a dsRNA-specific affinity resi...

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Bibliographic Details
Main Authors:	Nathaniel E. Clark, Mateusz Kozarski, Sinem Demirel Asci, Jurgen Van den Heuvel, Matt R. Schraut, Roger A. Winters, Kelley Kearns, Thomas C. Scanlon, Senne Dillen
Format:	Article
Language:	English
Published:	Elsevier 2025-06-01
Series:	Molecular Therapy: Nucleic Acids
Subjects:	MT: Oligonucleotides: Therapies and Applications double-stranded RNA messenger RNA circular RNA self-amplifying RNA in vitro transcription
Online Access:	http://www.sciencedirect.com/science/article/pii/S2162253125001039
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Removal of dsRNA byproducts using affinity chromatography

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