‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic Channels
Microfluidic sensors incorporated onto chips allow sensor miniaturization and high-throughput analyses for point-of-care or non-clinical analytical tools. Three-dimensional (3D) printing based on femtosecond laser direct writing (fs-LDW) is useful for creating 3D microstructures with high spatial re...
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MDPI AG
2025-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/15/2/104 |
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| author | Daniela Serien Koji Sugioka Aiko Narazaki |
| author_facet | Daniela Serien Koji Sugioka Aiko Narazaki |
| author_sort | Daniela Serien |
| collection | DOAJ |
| description | Microfluidic sensors incorporated onto chips allow sensor miniaturization and high-throughput analyses for point-of-care or non-clinical analytical tools. Three-dimensional (3D) printing based on femtosecond laser direct writing (fs-LDW) is useful for creating 3D microstructures with high spatial resolution because the structures are printed in 3D space along a designated laser light path. High-performance biochips can be fabricated using the ‘ship-in-a-bottle’ integration technique, in which functional microcomponents or biomimetic structures are embedded inside closed microchannels using fs-LDW. Solutions containing protein biomacromolecules as a precursor can be used to fabricate microstructures that retain their native protein functions. Here, we demonstrate the ship-in-a-bottle integration of pure 3D proteinaceous microstructures that exhibit pH sensitivity. We fabricated proteinaceous mesh structures with gap sizes of 10 and 5 μm. The sizes of these gaps changed when exposed to physiological buffers ranging from pH of 4 to 10. The size of the gaps in the mesh can be shrunk and expanded repeatedly by changing the pH of the surrounding buffer. Fs-LDW enables the construction of microscopic proteinaceous meshes that exhibit dynamic functions such as pH sensing and might find applications for filtering particles in microfluidic channels. |
| format | Article |
| id | doaj-art-6e98fbcb56cb4d2bac0f7be1adb84d61 |
| institution | Kabale University |
| issn | 2079-4991 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj-art-6e98fbcb56cb4d2bac0f7be1adb84d612025-01-24T13:44:09ZengMDPI AGNanomaterials2079-49912025-01-0115210410.3390/nano15020104‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic ChannelsDaniela Serien0Koji Sugioka1Aiko Narazaki2Innovative Laser Processing Group, Research Institute for Advanced Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Ibaraki, JapanAdvanced Laser Processing Research Team, RIKEN Center for Advanced Photonics, Wako 351-0198, Saitama, JapanInnovative Laser Processing Group, Research Institute for Advanced Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Ibaraki, JapanMicrofluidic sensors incorporated onto chips allow sensor miniaturization and high-throughput analyses for point-of-care or non-clinical analytical tools. Three-dimensional (3D) printing based on femtosecond laser direct writing (fs-LDW) is useful for creating 3D microstructures with high spatial resolution because the structures are printed in 3D space along a designated laser light path. High-performance biochips can be fabricated using the ‘ship-in-a-bottle’ integration technique, in which functional microcomponents or biomimetic structures are embedded inside closed microchannels using fs-LDW. Solutions containing protein biomacromolecules as a precursor can be used to fabricate microstructures that retain their native protein functions. Here, we demonstrate the ship-in-a-bottle integration of pure 3D proteinaceous microstructures that exhibit pH sensitivity. We fabricated proteinaceous mesh structures with gap sizes of 10 and 5 μm. The sizes of these gaps changed when exposed to physiological buffers ranging from pH of 4 to 10. The size of the gaps in the mesh can be shrunk and expanded repeatedly by changing the pH of the surrounding buffer. Fs-LDW enables the construction of microscopic proteinaceous meshes that exhibit dynamic functions such as pH sensing and might find applications for filtering particles in microfluidic channels.https://www.mdpi.com/2079-4991/15/2/104femtosecond laser direct writing3D printingmicrofluidic integrationpH-actuation |
| spellingShingle | Daniela Serien Koji Sugioka Aiko Narazaki ‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic Channels Nanomaterials femtosecond laser direct writing 3D printing microfluidic integration pH-actuation |
| title | ‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic Channels |
| title_full | ‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic Channels |
| title_fullStr | ‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic Channels |
| title_full_unstemmed | ‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic Channels |
| title_short | ‘Ship-in-a-Bottle’ Integration of pH-Sensitive 3D Proteinaceous Meshes into Microfluidic Channels |
| title_sort | ship in a bottle integration of ph sensitive 3d proteinaceous meshes into microfluidic channels |
| topic | femtosecond laser direct writing 3D printing microfluidic integration pH-actuation |
| url | https://www.mdpi.com/2079-4991/15/2/104 |
| work_keys_str_mv | AT danielaserien shipinabottleintegrationofphsensitive3dproteinaceousmeshesintomicrofluidicchannels AT kojisugioka shipinabottleintegrationofphsensitive3dproteinaceousmeshesintomicrofluidicchannels AT aikonarazaki shipinabottleintegrationofphsensitive3dproteinaceousmeshesintomicrofluidicchannels |