5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells
5-Aminolevulinic acid hydrochloride (ALA)-mediated sonodynamic therapy (SDT) had anti-tumour effect on pancreatic cancer cells. Hence, ALA loaded lipid/poly(lactic-co-glycolic acid) (PLGA) microbubbles (MBs)-mediated SDT for pancreatic cancer has great potential. The average size of ALA-lipid MBs an...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1813743 |
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| author | Wei Yang Haitao Xu Qinghao Liu Chunxun Liu Jiahe Hu Ping Liu Tianyi Fang Yuxian Bai Jiuxin Zhu Rui Xie |
| author_facet | Wei Yang Haitao Xu Qinghao Liu Chunxun Liu Jiahe Hu Ping Liu Tianyi Fang Yuxian Bai Jiuxin Zhu Rui Xie |
| author_sort | Wei Yang |
| collection | DOAJ |
| description | 5-Aminolevulinic acid hydrochloride (ALA)-mediated sonodynamic therapy (SDT) had anti-tumour effect on pancreatic cancer cells. Hence, ALA loaded lipid/poly(lactic-co-glycolic acid) (PLGA) microbubbles (MBs)-mediated SDT for pancreatic cancer has great potential. The average size of ALA-lipid MBs and ALA-PLGA MBs was about 3.0 µm. The two kinds of MBs had good biocompatibility to normal HPDE6-C7 cells and were not toxic to pancreatic cancer cells. Compared with ALA-induced SDT, a statistically significant decrease in cell viability was observed in ALA lipid/PLGA MBs combined with ultrasound groups in AsPC-1 and BxPC-3 cells (p < .05). Obvious effect on the apoptotic rate, apoptosis and pyroptosis morphology, enhanced reactive oxygen species was found in ALA-lipid/PLGA MBs mediated SDT in vitro. Through in vivo study, we found ALA-lipid/PLGA MBs-mediated SDT was a promise treatment for pancreatic cancer. |
| format | Article |
| id | doaj-art-6e7d03bc97da4562a9d1e7c3c2ebc9c8 |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-6e7d03bc97da4562a9d1e7c3c2ebc9c82025-08-20T03:41:25ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-014811178118810.1080/21691401.2020.18137435-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cellsWei Yang0Haitao Xu1Qinghao Liu2Chunxun Liu3Jiahe Hu4Ping Liu5Tianyi Fang6Yuxian Bai7Jiuxin Zhu8Rui Xie9Harbin Medical University Cancer Hospital, Harbin, ChinaHarbin Medical University Cancer Hospital, Harbin, ChinaHarbin Medical University Cancer Hospital, Harbin, ChinaHarbin Medical University Cancer Hospital, Harbin, ChinaHarbin Medical University Cancer Hospital, Harbin, ChinaHarbin Medical University Cancer Hospital, Harbin, ChinaHarbin Medical University Cancer Hospital, Harbin, ChinaHarbin Medical University Cancer Hospital, Harbin, ChinaDepartment of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratories of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, ChinaHarbin Medical University Cancer Hospital, Harbin, China5-Aminolevulinic acid hydrochloride (ALA)-mediated sonodynamic therapy (SDT) had anti-tumour effect on pancreatic cancer cells. Hence, ALA loaded lipid/poly(lactic-co-glycolic acid) (PLGA) microbubbles (MBs)-mediated SDT for pancreatic cancer has great potential. The average size of ALA-lipid MBs and ALA-PLGA MBs was about 3.0 µm. The two kinds of MBs had good biocompatibility to normal HPDE6-C7 cells and were not toxic to pancreatic cancer cells. Compared with ALA-induced SDT, a statistically significant decrease in cell viability was observed in ALA lipid/PLGA MBs combined with ultrasound groups in AsPC-1 and BxPC-3 cells (p < .05). Obvious effect on the apoptotic rate, apoptosis and pyroptosis morphology, enhanced reactive oxygen species was found in ALA-lipid/PLGA MBs mediated SDT in vitro. Through in vivo study, we found ALA-lipid/PLGA MBs-mediated SDT was a promise treatment for pancreatic cancer.https://www.tandfonline.com/doi/10.1080/21691401.2020.1813743Pancreatic cancersonodynamic therapy5-aminolevulinic acidmicrobubblesapoptosispyroptosis |
| spellingShingle | Wei Yang Haitao Xu Qinghao Liu Chunxun Liu Jiahe Hu Ping Liu Tianyi Fang Yuxian Bai Jiuxin Zhu Rui Xie 5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells Artificial Cells, Nanomedicine, and Biotechnology Pancreatic cancer sonodynamic therapy 5-aminolevulinic acid microbubbles apoptosis pyroptosis |
| title | 5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells |
| title_full | 5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells |
| title_fullStr | 5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells |
| title_full_unstemmed | 5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells |
| title_short | 5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells |
| title_sort | 5 aminolevulinic acid hydrochloride loaded microbubbles mediated sonodynamic therapy in pancreatic cancer cells |
| topic | Pancreatic cancer sonodynamic therapy 5-aminolevulinic acid microbubbles apoptosis pyroptosis |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1813743 |
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