Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem
ABSTRACT Environmental DNA (eDNA) offers an efficient approach to biodiversity monitoring and biosecurity surveillance in coastal ecosystems. Understanding eDNA dynamics in tidal areas is critical for guiding sampling strategy design and interpreting molecular detection results. In a field study con...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Environmental DNA |
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| Online Access: | https://doi.org/10.1002/edn3.70039 |
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| author | Michelle Scriver Ulla vonAmmon Xavier Pochon Martin Zirngibl Francois Audrezet Kaeden Leonard Kylie Pedersen Nick Bamford Ngairo Tahere Jo‐Ann L. Stanton Neil J. Gemmell Anastasija Zaiko |
| author_facet | Michelle Scriver Ulla vonAmmon Xavier Pochon Martin Zirngibl Francois Audrezet Kaeden Leonard Kylie Pedersen Nick Bamford Ngairo Tahere Jo‐Ann L. Stanton Neil J. Gemmell Anastasija Zaiko |
| author_sort | Michelle Scriver |
| collection | DOAJ |
| description | ABSTRACT Environmental DNA (eDNA) offers an efficient approach to biodiversity monitoring and biosecurity surveillance in coastal ecosystems. Understanding eDNA dynamics in tidal areas is critical for guiding sampling strategy design and interpreting molecular detection results. In a field study conducted at Ōpua, Bay of Islands, Aotearoa, New Zealand, an international marina with a dynamic coastal environment, we investigated eDNA detection patterns across a local spatial and temporal gradient. Hourly samples were collected from six sampling stations around the marina, from artificial shore structures and a channel, over a 12‐h tidal cycle. Detected eukaryotic communities were assessed using eDNA metabarcoding analysis of the small‐subunit ribosomal RNA (18S rRNA) and mitochondrial Cytochrome C Oxidase subunit I (COI) genes. This core community was screened for marine nonindigenous species (NIS) using an online metabarcoding data screening app (ExPAT). Community composition varied significantly based on sampling location and time, with most of the core community captured around mid‐tide. The NIS community exhibited inconsistent detection for some species (e.g., the amphipod Monocorophium acherusicum) across space and time, whereas other NIS (e.g., the mussel Arcuatula senhousia) were consistently detected and behaved similar to the core community. Overall, species‐specific eDNA detection patterns may be linked to the biology and movement of the organism. The results indicated slightly higher NIS detection from artificial structures at the shore stations and within 2 h of low tide. For marine biosecurity applications, users may want to prioritize sampling near‐shore during low tide, while samples collected during mid‐tide may provide more comprehensive insights into the core community for biomonitoring. Further field studies across multiple tidal cycles and marinas may guide the standardization of molecular biomonitoring and biosurveillance sampling strategies and support their broader integration into marine biosecurity programs. |
| format | Article |
| id | doaj-art-6e79dd5f4e9144e693dfd801b48d50cb |
| institution | Kabale University |
| issn | 2637-4943 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Environmental DNA |
| spelling | doaj-art-6e79dd5f4e9144e693dfd801b48d50cb2025-01-29T05:11:50ZengWileyEnvironmental DNA2637-49432024-11-0166n/an/a10.1002/edn3.70039Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal EcosystemMichelle Scriver0Ulla vonAmmon1Xavier Pochon2Martin Zirngibl3Francois Audrezet4Kaeden Leonard5Kylie Pedersen6Nick Bamford7Ngairo Tahere8Jo‐Ann L. Stanton9Neil J. Gemmell10Anastasija Zaiko11Biosecurity Group Cawthron Institute Nelson New ZealandBiosecurity Group Cawthron Institute Nelson New ZealandBiosecurity Group Cawthron Institute Nelson New ZealandBiosecurity Group Cawthron Institute Nelson New ZealandBiosecurity Group Cawthron Institute Nelson New ZealandNorthland Regional Council Whangārei New ZealandNorthland Regional Council Whangārei New ZealandNorthland Regional Council Whangārei New ZealandPōkai o Ngāti Manu Karetu New ZealandDepartment of Anatomy University of Otago Dunedin New ZealandDepartment of Anatomy University of Otago Dunedin New ZealandBiosecurity Group Cawthron Institute Nelson New ZealandABSTRACT Environmental DNA (eDNA) offers an efficient approach to biodiversity monitoring and biosecurity surveillance in coastal ecosystems. Understanding eDNA dynamics in tidal areas is critical for guiding sampling strategy design and interpreting molecular detection results. In a field study conducted at Ōpua, Bay of Islands, Aotearoa, New Zealand, an international marina with a dynamic coastal environment, we investigated eDNA detection patterns across a local spatial and temporal gradient. Hourly samples were collected from six sampling stations around the marina, from artificial shore structures and a channel, over a 12‐h tidal cycle. Detected eukaryotic communities were assessed using eDNA metabarcoding analysis of the small‐subunit ribosomal RNA (18S rRNA) and mitochondrial Cytochrome C Oxidase subunit I (COI) genes. This core community was screened for marine nonindigenous species (NIS) using an online metabarcoding data screening app (ExPAT). Community composition varied significantly based on sampling location and time, with most of the core community captured around mid‐tide. The NIS community exhibited inconsistent detection for some species (e.g., the amphipod Monocorophium acherusicum) across space and time, whereas other NIS (e.g., the mussel Arcuatula senhousia) were consistently detected and behaved similar to the core community. Overall, species‐specific eDNA detection patterns may be linked to the biology and movement of the organism. The results indicated slightly higher NIS detection from artificial structures at the shore stations and within 2 h of low tide. For marine biosecurity applications, users may want to prioritize sampling near‐shore during low tide, while samples collected during mid‐tide may provide more comprehensive insights into the core community for biomonitoring. Further field studies across multiple tidal cycles and marinas may guide the standardization of molecular biomonitoring and biosurveillance sampling strategies and support their broader integration into marine biosecurity programs.https://doi.org/10.1002/edn3.70039biomonitoring screeningenvironmental DNAmarine biosecuritymetabarcodingnonindigenous speciesspatiotemporal gradient |
| spellingShingle | Michelle Scriver Ulla vonAmmon Xavier Pochon Martin Zirngibl Francois Audrezet Kaeden Leonard Kylie Pedersen Nick Bamford Ngairo Tahere Jo‐Ann L. Stanton Neil J. Gemmell Anastasija Zaiko Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem Environmental DNA biomonitoring screening environmental DNA marine biosecurity metabarcoding nonindigenous species spatiotemporal gradient |
| title | Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem |
| title_full | Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem |
| title_fullStr | Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem |
| title_full_unstemmed | Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem |
| title_short | Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem |
| title_sort | ebbs and flows of marine biodiversity navigating spatiotemporal patterns of environmental dna in a coastal tidal ecosystem |
| topic | biomonitoring screening environmental DNA marine biosecurity metabarcoding nonindigenous species spatiotemporal gradient |
| url | https://doi.org/10.1002/edn3.70039 |
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