Effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0-21 d old broilers

A study evaluated the effects of limestone sources with different solubility on the efficacy of two phytases in broilers. A 2×5 factorial arrangement was employed with two commercial limestone sources and five diet treatments; a positive control (PC) diet with 7.2 g/kg P and 9.6 g/kg Ca, and a negat...

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Main Authors: R. Davin, C. Kwakernaak, Y. Dersjant-Li
Format: Article
Language:English
Published: Brill 2020-08-01
Series:Journal of Applied Animal Nutrition
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Online Access:https://www.wageningenacademic.com/doi/10.3920/JAAN2020.0003
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author R. Davin
C. Kwakernaak
Y. Dersjant-Li
author_facet R. Davin
C. Kwakernaak
Y. Dersjant-Li
author_sort R. Davin
collection DOAJ
description A study evaluated the effects of limestone sources with different solubility on the efficacy of two phytases in broilers. A 2×5 factorial arrangement was employed with two commercial limestone sources and five diet treatments; a positive control (PC) diet with 7.2 g/kg P and 9.6 g/kg Ca, and a negative control diet containing reductions of 1.87 g/kg available P, 1.99 g/kg Ca and 0.4 g/kg Na, supplemented with either Buttiauxella phytase (PhyB) or Escherichia coli phytase (PhyE) at 500 or 1000 FTU/kg diet. The two limestone sources were feed-grade commercial products with different particle sizes and solubility (fast-soluble (FS) 100%; slow-soluble (SS) 26% soluble after 30 minutes at pH 3) containing similar levels of Ca. Diets were fed to one-day-old Ross 308 males (n=2,400) with 30 birds/pen and eight pens/treatment in two phases (starter 0-10 d and grower 10-21 d). On d 21, ileal digesta was collected from 12 birds/pen to determine apparent ileal digestibility (AID) of P and Ca, and myo-inositol hexakisphosphate (IP6) disappearance, and tibias from four birds/pen for ash determination. The SS limestone improved body weight gain (BWG), feed intake (FI) and FCR vs FS limestone in starter/grower phases (P<0.05), and improved AID of P (P<0.05) and IP6 disappearance (P<0.05) at d 21. There was an interaction between limestone and phytase on BWG, FI and FCR in the grower phase (P≤0.05) whereby FS (vs SS) limestone reduced BWG at either dose of PhyE, but only at 500 FTU/kg of PhyB. At an equivalent dose, PhyB had higher BWG and feed intake than PhyE (P<0.05). At 1000 FTU/kg, performance was equivalent (BWG and FI) or superior (FCR) to the PC, PhyB produced greater tibia ash, AID of P and IP6 disappearance (P<0.05). The findings showed that the effects of limestone particle size on phytase efficacy varied with phytase source and dose.
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spelling doaj-art-6e708bda6ecb463289124e1300f581862024-12-02T08:44:35ZengBrillJournal of Applied Animal Nutrition2049-257X2020-08-0182617310.3920/JAAN2020.0003Effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0-21 d old broilersR. Davin0C. Kwakernaak1Y. Dersjant-Li2Schothorst Feed Research, P.O. Box 553, 8200 AM Lelystad, the Netherlands.Schothorst Feed Research, P.O. Box 553, 8200 AM Lelystad, the Netherlands.DuPont Animal Nutrition, DuPont Nutrition and Biosciences, Archimedesweg 30, 2333 CN Leiden, the Netherlands.A study evaluated the effects of limestone sources with different solubility on the efficacy of two phytases in broilers. A 2×5 factorial arrangement was employed with two commercial limestone sources and five diet treatments; a positive control (PC) diet with 7.2 g/kg P and 9.6 g/kg Ca, and a negative control diet containing reductions of 1.87 g/kg available P, 1.99 g/kg Ca and 0.4 g/kg Na, supplemented with either Buttiauxella phytase (PhyB) or Escherichia coli phytase (PhyE) at 500 or 1000 FTU/kg diet. The two limestone sources were feed-grade commercial products with different particle sizes and solubility (fast-soluble (FS) 100%; slow-soluble (SS) 26% soluble after 30 minutes at pH 3) containing similar levels of Ca. Diets were fed to one-day-old Ross 308 males (n=2,400) with 30 birds/pen and eight pens/treatment in two phases (starter 0-10 d and grower 10-21 d). On d 21, ileal digesta was collected from 12 birds/pen to determine apparent ileal digestibility (AID) of P and Ca, and myo-inositol hexakisphosphate (IP6) disappearance, and tibias from four birds/pen for ash determination. The SS limestone improved body weight gain (BWG), feed intake (FI) and FCR vs FS limestone in starter/grower phases (P<0.05), and improved AID of P (P<0.05) and IP6 disappearance (P<0.05) at d 21. There was an interaction between limestone and phytase on BWG, FI and FCR in the grower phase (P≤0.05) whereby FS (vs SS) limestone reduced BWG at either dose of PhyE, but only at 500 FTU/kg of PhyB. At an equivalent dose, PhyB had higher BWG and feed intake than PhyE (P<0.05). At 1000 FTU/kg, performance was equivalent (BWG and FI) or superior (FCR) to the PC, PhyB produced greater tibia ash, AID of P and IP6 disappearance (P<0.05). The findings showed that the effects of limestone particle size on phytase efficacy varied with phytase source and dose.https://www.wageningenacademic.com/doi/10.3920/JAAN2020.0003broiler chickenscalciumcalcium carbonatephosphorusphytase
spellingShingle R. Davin
C. Kwakernaak
Y. Dersjant-Li
Effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0-21 d old broilers
Journal of Applied Animal Nutrition
broiler chickens
calcium
calcium carbonate
phosphorus
phytase
title Effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0-21 d old broilers
title_full Effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0-21 d old broilers
title_fullStr Effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0-21 d old broilers
title_full_unstemmed Effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0-21 d old broilers
title_short Effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0-21 d old broilers
title_sort effect of two commercial limestone sources with different solubility on the efficacy of two phytases in 0 21 d old broilers
topic broiler chickens
calcium
calcium carbonate
phosphorus
phytase
url https://www.wageningenacademic.com/doi/10.3920/JAAN2020.0003
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AT ckwakernaak effectoftwocommerciallimestonesourceswithdifferentsolubilityontheefficacyoftwophytasesin021doldbroilers
AT ydersjantli effectoftwocommerciallimestonesourceswithdifferentsolubilityontheefficacyoftwophytasesin021doldbroilers