Inhibitory Effect of <i>Moringa oleifera</i> Seed Extract and Its Behenic Acid Component on <i>Staphylococcus aureus</i> Biofilm Formation
<b>Background/Objectives:</b> Inhibiting biofilm formation without killing cells facilitates the physical removal of contaminating bacteria while minimizing the opportunity for resistant bacteria to emerge. <b>Results:</b> The <i>M</i>. <i>oleifera</i>...
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MDPI AG
2024-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/14/1/19 |
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| author | Seoyoung Kim Tae-Jong Kim |
| author_facet | Seoyoung Kim Tae-Jong Kim |
| author_sort | Seoyoung Kim |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Inhibiting biofilm formation without killing cells facilitates the physical removal of contaminating bacteria while minimizing the opportunity for resistant bacteria to emerge. <b>Results:</b> The <i>M</i>. <i>oleifera</i> methanolic seed extract contained 1.48% behenic acid, significantly inhibiting <i>S</i>. <i>aureus</i> biofilm formation. Although behenic acid did not affect cell growth, it inhibited biofilm formation in a concentration-dependent manner, up to 20 mg/L. The cell physiology changes caused by behenic acid are potentially unrelated to biofilm formation inhibition, as no correlation was noted between cell hydrophobicity, polysaccharide production, extracellular DNA production, or protein production and behenic acid concentration. Thus, it was hypothesized that the surfactant properties of behenic acid contribute to its ability to inhibit biofilm formation, as a similar biofilm-inhibitory effect was observed when <i>S. aureus</i> was administered 1% Tween 80, a surfactant. <b>Methods</b>: A methanolic extract of <i>Moringa oleifera</i> seeds was selected from a library of edible plant extracts to inhibit <i>Staphylococcus aureus</i> biofilm formation without cell killing. <b>Conclusions</b>: Behenic acid is a saturated fatty acid that is used as an ingredient in cosmetics and ointments; thus, behenic acid may benefit the skin by inhibiting the biofilm formation of <i>S</i>. <i>aureus</i>, a commensal skin pathogen. |
| format | Article |
| id | doaj-art-6e669e5a4489456090fdb13faade08c4 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-6e669e5a4489456090fdb13faade08c42025-01-24T13:18:34ZengMDPI AGAntibiotics2079-63822024-12-011411910.3390/antibiotics14010019Inhibitory Effect of <i>Moringa oleifera</i> Seed Extract and Its Behenic Acid Component on <i>Staphylococcus aureus</i> Biofilm FormationSeoyoung Kim0Tae-Jong Kim1Department of Forest Products and Biotechnology, Kookmin University, Seoul 02707, Republic of KoreaDepartment of Forest Products and Biotechnology, Kookmin University, Seoul 02707, Republic of Korea<b>Background/Objectives:</b> Inhibiting biofilm formation without killing cells facilitates the physical removal of contaminating bacteria while minimizing the opportunity for resistant bacteria to emerge. <b>Results:</b> The <i>M</i>. <i>oleifera</i> methanolic seed extract contained 1.48% behenic acid, significantly inhibiting <i>S</i>. <i>aureus</i> biofilm formation. Although behenic acid did not affect cell growth, it inhibited biofilm formation in a concentration-dependent manner, up to 20 mg/L. The cell physiology changes caused by behenic acid are potentially unrelated to biofilm formation inhibition, as no correlation was noted between cell hydrophobicity, polysaccharide production, extracellular DNA production, or protein production and behenic acid concentration. Thus, it was hypothesized that the surfactant properties of behenic acid contribute to its ability to inhibit biofilm formation, as a similar biofilm-inhibitory effect was observed when <i>S. aureus</i> was administered 1% Tween 80, a surfactant. <b>Methods</b>: A methanolic extract of <i>Moringa oleifera</i> seeds was selected from a library of edible plant extracts to inhibit <i>Staphylococcus aureus</i> biofilm formation without cell killing. <b>Conclusions</b>: Behenic acid is a saturated fatty acid that is used as an ingredient in cosmetics and ointments; thus, behenic acid may benefit the skin by inhibiting the biofilm formation of <i>S</i>. <i>aureus</i>, a commensal skin pathogen.https://www.mdpi.com/2079-6382/14/1/19behenic acidbiofilm<i>Moringa oleifera</i> seed<i>Staphylococcus aureus</i> |
| spellingShingle | Seoyoung Kim Tae-Jong Kim Inhibitory Effect of <i>Moringa oleifera</i> Seed Extract and Its Behenic Acid Component on <i>Staphylococcus aureus</i> Biofilm Formation Antibiotics behenic acid biofilm <i>Moringa oleifera</i> seed <i>Staphylococcus aureus</i> |
| title | Inhibitory Effect of <i>Moringa oleifera</i> Seed Extract and Its Behenic Acid Component on <i>Staphylococcus aureus</i> Biofilm Formation |
| title_full | Inhibitory Effect of <i>Moringa oleifera</i> Seed Extract and Its Behenic Acid Component on <i>Staphylococcus aureus</i> Biofilm Formation |
| title_fullStr | Inhibitory Effect of <i>Moringa oleifera</i> Seed Extract and Its Behenic Acid Component on <i>Staphylococcus aureus</i> Biofilm Formation |
| title_full_unstemmed | Inhibitory Effect of <i>Moringa oleifera</i> Seed Extract and Its Behenic Acid Component on <i>Staphylococcus aureus</i> Biofilm Formation |
| title_short | Inhibitory Effect of <i>Moringa oleifera</i> Seed Extract and Its Behenic Acid Component on <i>Staphylococcus aureus</i> Biofilm Formation |
| title_sort | inhibitory effect of i moringa oleifera i seed extract and its behenic acid component on i staphylococcus aureus i biofilm formation |
| topic | behenic acid biofilm <i>Moringa oleifera</i> seed <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/2079-6382/14/1/19 |
| work_keys_str_mv | AT seoyoungkim inhibitoryeffectofimoringaoleiferaiseedextractanditsbehenicacidcomponentonistaphylococcusaureusibiofilmformation AT taejongkim inhibitoryeffectofimoringaoleiferaiseedextractanditsbehenicacidcomponentonistaphylococcusaureusibiofilmformation |