The relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysis

Abstract Background Atherosclerosis (AS) poses a pressing challenge in contemporary medicine. Glycolysis is a crucial bioenergetic metabolic pathway that provides the primary energy source for endothelial cells. Resveratrol (Res) is a natural compound that has been shown to possess AS. However, the...

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Main Authors: Henan Pan, Zongkai Wu, Yaran Gao, Wentao Yao, Ge Feng, Hebo Wang
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Cardiovascular Disorders
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Online Access:https://doi.org/10.1186/s12872-025-04735-3
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author Henan Pan
Zongkai Wu
Yaran Gao
Wentao Yao
Ge Feng
Hebo Wang
author_facet Henan Pan
Zongkai Wu
Yaran Gao
Wentao Yao
Ge Feng
Hebo Wang
author_sort Henan Pan
collection DOAJ
description Abstract Background Atherosclerosis (AS) poses a pressing challenge in contemporary medicine. Glycolysis is a crucial bioenergetic metabolic pathway that provides the primary energy source for endothelial cells. Resveratrol (Res) is a natural compound that has been shown to possess AS. However, the underlying mechanisms of its anti-atherosclerotic effects are not yet fully understood. Methods We established a balloon injury model of the common carotid artery in Sprague-Dawley (SD) rats and an ox-LDL endothelial cell injury model for in vivo and in vitro experiments, respectively. Results Our study showed that 14 days after balloon-induced injury to the carotid intima of SD rats in vitro, the levels of glycolysis-related proteins fructose-2,6-bisphosphatase 3 (PFKFB3), glucose transporter 1 (GLUT1) and hexokinase 2 (HK2) were increased. Meanwhile, Res treatment improved intimal hyperplasia and reduced the levels of expression of these glycolysis-related proteins, and with higher concentrations of Res leading to more pronounced improvements. In vivo, in ox-LDL HUVECs, Res reduced glucose uptake and lactate production, inhibited apoptosis, and decreased the expression of PFKFB3, GLUT1, HK2, and p-AKT. After the addition of a phosphatidylinositol 3-kinase (PI3K) inhibitor, the we established a balloon injury model of the common carotid artery in SD rats and an ox-LDL endothelial cell injury model for in vivo and in vitro experiments, respectively, and expression levels of p-AKT were observed to increase. Conclusion According to these findings, Resveratrol can reduce AS by influencing glycolysis and inhibiting apoptosis through the PI3K-AKT signalling pathway.
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spelling doaj-art-6e636c792f3e43aaa46ac1c2551a2b262025-08-20T02:30:19ZengBMCBMC Cardiovascular Disorders1471-22612025-04-0125111410.1186/s12872-025-04735-3The relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysisHenan Pan0Zongkai Wu1Yaran Gao2Wentao Yao3Ge Feng4Hebo Wang5Department of Graduate School, Hebei Medical UniversityDepartment of Neurology, Hebei General Hospital, Affiliated to Hebei Medical UniversityDepartment of Neurology, Hebei General Hospital, Affiliated to Hebei Medical UniversityDepartment of Graduate School, Hebei Medical UniversityDepartment of Graduate School, Hebei Medical UniversityDepartment of Graduate School, Hebei Medical UniversityAbstract Background Atherosclerosis (AS) poses a pressing challenge in contemporary medicine. Glycolysis is a crucial bioenergetic metabolic pathway that provides the primary energy source for endothelial cells. Resveratrol (Res) is a natural compound that has been shown to possess AS. However, the underlying mechanisms of its anti-atherosclerotic effects are not yet fully understood. Methods We established a balloon injury model of the common carotid artery in Sprague-Dawley (SD) rats and an ox-LDL endothelial cell injury model for in vivo and in vitro experiments, respectively. Results Our study showed that 14 days after balloon-induced injury to the carotid intima of SD rats in vitro, the levels of glycolysis-related proteins fructose-2,6-bisphosphatase 3 (PFKFB3), glucose transporter 1 (GLUT1) and hexokinase 2 (HK2) were increased. Meanwhile, Res treatment improved intimal hyperplasia and reduced the levels of expression of these glycolysis-related proteins, and with higher concentrations of Res leading to more pronounced improvements. In vivo, in ox-LDL HUVECs, Res reduced glucose uptake and lactate production, inhibited apoptosis, and decreased the expression of PFKFB3, GLUT1, HK2, and p-AKT. After the addition of a phosphatidylinositol 3-kinase (PI3K) inhibitor, the we established a balloon injury model of the common carotid artery in SD rats and an ox-LDL endothelial cell injury model for in vivo and in vitro experiments, respectively, and expression levels of p-AKT were observed to increase. Conclusion According to these findings, Resveratrol can reduce AS by influencing glycolysis and inhibiting apoptosis through the PI3K-AKT signalling pathway.https://doi.org/10.1186/s12872-025-04735-3GlycolysisAtherosclerosisVascular diseasesResveratrolPI3K-Akt
spellingShingle Henan Pan
Zongkai Wu
Yaran Gao
Wentao Yao
Ge Feng
Hebo Wang
The relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysis
BMC Cardiovascular Disorders
Glycolysis
Atherosclerosis
Vascular diseases
Resveratrol
PI3K-Akt
title The relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysis
title_full The relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysis
title_fullStr The relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysis
title_full_unstemmed The relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysis
title_short The relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysis
title_sort relevance of resveratrol in ameliorating carotid atherosclerosis through glycolysis
topic Glycolysis
Atherosclerosis
Vascular diseases
Resveratrol
PI3K-Akt
url https://doi.org/10.1186/s12872-025-04735-3
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