Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance of Ajuga multiflora Bunge by Altering Activity of Antioxidant Enzyme
We investigated the effect of Si concentration on shoot regeneration and salinity tolerance of Ajuga multiflora. Addition of Si to the shoot induction medium significantly increased the frequency of shoot induction. The average number of shoots regenerated per explant decreased on the medium contain...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/521703 |
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| author | Iyyakkannu Sivanesan Byoung Ryong Jeong |
| author_facet | Iyyakkannu Sivanesan Byoung Ryong Jeong |
| author_sort | Iyyakkannu Sivanesan |
| collection | DOAJ |
| description | We investigated the effect of Si concentration on shoot regeneration and salinity tolerance of Ajuga multiflora. Addition of Si to the shoot induction medium significantly increased the frequency of shoot induction. The average number of shoots regenerated per explant decreased on the medium containing NaCl alone, while there was less decrease when the shoot induction medium was supplemented with both NaCl and Si. The shoot induction percentage increased linearly with increasing concentration of Si in the NaCl containing medium. Addition of Si to the shoot induction medium significantly increased SOD, POD, APX, and CAT activity in regenerated shoot buds as compared with the control. The inclusion of Si to the NaCl containing medium significantly increased the SOD activity in leaves and roots, while it decreased POD, APX, and CAT activity in both organs. Scanning electron microscopic analysis showed that there are no distinct differences in the structure of stomata between the control and Si-treated plants. However, NaCl treatment significantly affected the structure and number of stomata as compared to the control. Wavelength dispersive X-ray analysis confirmed the high Si deposition in trichomes of plants grown in the Si containing medium but not in plants grown in the medium without Si. |
| format | Article |
| id | doaj-art-6e53e319cced40e88478c3d4cefce445 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-6e53e319cced40e88478c3d4cefce4452025-02-03T05:48:19ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/521703521703Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance of Ajuga multiflora Bunge by Altering Activity of Antioxidant EnzymeIyyakkannu Sivanesan0Byoung Ryong Jeong1Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaInstitute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaWe investigated the effect of Si concentration on shoot regeneration and salinity tolerance of Ajuga multiflora. Addition of Si to the shoot induction medium significantly increased the frequency of shoot induction. The average number of shoots regenerated per explant decreased on the medium containing NaCl alone, while there was less decrease when the shoot induction medium was supplemented with both NaCl and Si. The shoot induction percentage increased linearly with increasing concentration of Si in the NaCl containing medium. Addition of Si to the shoot induction medium significantly increased SOD, POD, APX, and CAT activity in regenerated shoot buds as compared with the control. The inclusion of Si to the NaCl containing medium significantly increased the SOD activity in leaves and roots, while it decreased POD, APX, and CAT activity in both organs. Scanning electron microscopic analysis showed that there are no distinct differences in the structure of stomata between the control and Si-treated plants. However, NaCl treatment significantly affected the structure and number of stomata as compared to the control. Wavelength dispersive X-ray analysis confirmed the high Si deposition in trichomes of plants grown in the Si containing medium but not in plants grown in the medium without Si.http://dx.doi.org/10.1155/2014/521703 |
| spellingShingle | Iyyakkannu Sivanesan Byoung Ryong Jeong Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance of Ajuga multiflora Bunge by Altering Activity of Antioxidant Enzyme The Scientific World Journal |
| title | Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance of Ajuga multiflora Bunge by Altering Activity of Antioxidant Enzyme |
| title_full | Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance of Ajuga multiflora Bunge by Altering Activity of Antioxidant Enzyme |
| title_fullStr | Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance of Ajuga multiflora Bunge by Altering Activity of Antioxidant Enzyme |
| title_full_unstemmed | Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance of Ajuga multiflora Bunge by Altering Activity of Antioxidant Enzyme |
| title_short | Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance of Ajuga multiflora Bunge by Altering Activity of Antioxidant Enzyme |
| title_sort | silicon promotes adventitious shoot regeneration and enhances salinity tolerance of ajuga multiflora bunge by altering activity of antioxidant enzyme |
| url | http://dx.doi.org/10.1155/2014/521703 |
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