Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications
Cyanobacteria, renowned for their photosynthetic capabilities, serve as efficient microbial chassis capable of converting carbon dioxide into a spectrum of bio-chemicals. However, conventional genetic manipulation strategies have proven incompatible with the precise and systematic modifications requ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1552030/full |
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| author | Yongjiu Zhang Shuxiao Yang Xianliang Zheng Xianliang Zheng Xianliang Zheng Xiaoming Tan |
| author_facet | Yongjiu Zhang Shuxiao Yang Xianliang Zheng Xianliang Zheng Xianliang Zheng Xiaoming Tan |
| author_sort | Yongjiu Zhang |
| collection | DOAJ |
| description | Cyanobacteria, renowned for their photosynthetic capabilities, serve as efficient microbial chassis capable of converting carbon dioxide into a spectrum of bio-chemicals. However, conventional genetic manipulation strategies have proven incompatible with the precise and systematic modifications required in the field of cyanobacterial synthetic biology. Here, we present an in-depth analysis of endogenous CRISPR-Cas systems within cyanobacterial genomes, with a particular focus on the Type I systems, which are the most widely distributed. We provide a comprehensive summary of the reported DNA defense mechanisms mediated by cyanobacterial Type I CRISPR-Cas systems and their current applications in genome editing. Furthermore, we offer insights into the future applications of these systems in the context of cyanobacterial genome editing, underscoring their potential to revolutionize synthetic biology approaches. |
| format | Article |
| id | doaj-art-6e2a39c73f554ca19fc0e30cbef5d4b3 |
| institution | OA Journals |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-6e2a39c73f554ca19fc0e30cbef5d4b32025-08-20T02:03:38ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-02-011310.3389/fbioe.2025.15520301552030Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applicationsYongjiu Zhang0Shuxiao Yang1Xianliang Zheng2Xianliang Zheng3Xianliang Zheng4Xiaoming Tan5State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, ChinaAngelYeast Co., Ltd., Yichang, Hubei, ChinaNational Key Laboratory of Agricultural Microbiology, AngelYeast Co., Ltd., Yichang, Hubei, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, ChinaCyanobacteria, renowned for their photosynthetic capabilities, serve as efficient microbial chassis capable of converting carbon dioxide into a spectrum of bio-chemicals. However, conventional genetic manipulation strategies have proven incompatible with the precise and systematic modifications required in the field of cyanobacterial synthetic biology. Here, we present an in-depth analysis of endogenous CRISPR-Cas systems within cyanobacterial genomes, with a particular focus on the Type I systems, which are the most widely distributed. We provide a comprehensive summary of the reported DNA defense mechanisms mediated by cyanobacterial Type I CRISPR-Cas systems and their current applications in genome editing. Furthermore, we offer insights into the future applications of these systems in the context of cyanobacterial genome editing, underscoring their potential to revolutionize synthetic biology approaches.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1552030/fulltype I CRISPR-Cascyanobacteriumgenome editingsynthetic biologymetabolic engineering |
| spellingShingle | Yongjiu Zhang Shuxiao Yang Xianliang Zheng Xianliang Zheng Xianliang Zheng Xiaoming Tan Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications Frontiers in Bioengineering and Biotechnology type I CRISPR-Cas cyanobacterium genome editing synthetic biology metabolic engineering |
| title | Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications |
| title_full | Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications |
| title_fullStr | Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications |
| title_full_unstemmed | Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications |
| title_short | Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications |
| title_sort | cyanobacterial type i crispr cas systems distribution mechanisms and genome editing applications |
| topic | type I CRISPR-Cas cyanobacterium genome editing synthetic biology metabolic engineering |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1552030/full |
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