Impact of a Plant Sterol Food Supplement on Eryptotic and Associated Cardiometabolic Parameters: A Randomized Placebo-Controlled Trial in Statin-Treated Patients

Eryptotic erythrocytes are prone to adhere to the vascular endothelium, provoking atherosclerosis. As statins do not prevent eryptosis compounds with anti-eryptotic effects could help treated hypercholesterolemic subjects in decreasing cardiovascular disease risk. Plant sterols (PSs) have shown this...

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Main Authors: Diego Miedes, Raquel Ortega-Luna, Sonia Broseta, Sergio Martínez-Hervás, Ángeles Álvarez-Ribelles, Víctor Collado-Díaz, Antonio Cilla, Amparo Alegría
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/13/24/4108
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author Diego Miedes
Raquel Ortega-Luna
Sonia Broseta
Sergio Martínez-Hervás
Ángeles Álvarez-Ribelles
Víctor Collado-Díaz
Antonio Cilla
Amparo Alegría
author_facet Diego Miedes
Raquel Ortega-Luna
Sonia Broseta
Sergio Martínez-Hervás
Ángeles Álvarez-Ribelles
Víctor Collado-Díaz
Antonio Cilla
Amparo Alegría
author_sort Diego Miedes
collection DOAJ
description Eryptotic erythrocytes are prone to adhere to the vascular endothelium, provoking atherosclerosis. As statins do not prevent eryptosis compounds with anti-eryptotic effects could help treated hypercholesterolemic subjects in decreasing cardiovascular disease risk. Plant sterols (PSs) have shown this anti-eryptotic effect ex vivo, along with their cholesterol-lowering activity. A parallel double-blind placebo-controlled randomized trial was conducted using a PS-food supplement (2 g of PS/day) (case, n = 13) or a placebo supplement (control, n = 13) in statin-treated hypercholesterolemic subjects. Blood samples were extracted before (T0) and after (T1) a 6-week treatment, and erythrocytes were isolated for biochemical determination, phosphatidylserine externalization (EPHS), cell size and reduced glutathione (GSH) analyses, and endothelium adhesion evaluation. A reduction in glucose (4.3%) and LDL cholesterol (9.2%) was observed only in the control group, whereas in the case group, an increase in ApoA1 (6.4%) was observed. Neither EPHS, cell size nor GSH were modified by the treatment with any of the supplements, whilst endothelium adhesion was reduced (55.1%) only in the case group. These results suggest that the PS supplement may improve some cardiovascular health parameters in the target population even though eryptosis status is not modified by this treatment.
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spelling doaj-art-6df9cfd6cd554d56beef76b6bb6ee2ce2025-08-20T02:00:27ZengMDPI AGFoods2304-81582024-12-011324410810.3390/foods13244108Impact of a Plant Sterol Food Supplement on Eryptotic and Associated Cardiometabolic Parameters: A Randomized Placebo-Controlled Trial in Statin-Treated PatientsDiego Miedes0Raquel Ortega-Luna1Sonia Broseta2Sergio Martínez-Hervás3Ángeles Álvarez-Ribelles4Víctor Collado-Díaz5Antonio Cilla6Amparo Alegría7Nutrition and Food Science Area, Faculty of Pharmacy and Food Sciences, University of Valencia, 46100 Valencia, SpainDepartment of Pharmacology, Faculty of Medicine, University of Valencia, 46010 Valencia, SpainNutrition and Food Science Area, Faculty of Pharmacy and Food Sciences, University of Valencia, 46100 Valencia, SpainEndocrinology and Nutrition Department, Hospital Clínico Universitario, 46010 Valencia, SpainDepartment of Pharmacology, Faculty of Medicine, University of Valencia, 46010 Valencia, SpainDepartment of Pharmacology, Faculty of Medicine, University of Valencia, 46010 Valencia, SpainNutrition and Food Science Area, Faculty of Pharmacy and Food Sciences, University of Valencia, 46100 Valencia, SpainNutrition and Food Science Area, Faculty of Pharmacy and Food Sciences, University of Valencia, 46100 Valencia, SpainEryptotic erythrocytes are prone to adhere to the vascular endothelium, provoking atherosclerosis. As statins do not prevent eryptosis compounds with anti-eryptotic effects could help treated hypercholesterolemic subjects in decreasing cardiovascular disease risk. Plant sterols (PSs) have shown this anti-eryptotic effect ex vivo, along with their cholesterol-lowering activity. A parallel double-blind placebo-controlled randomized trial was conducted using a PS-food supplement (2 g of PS/day) (case, n = 13) or a placebo supplement (control, n = 13) in statin-treated hypercholesterolemic subjects. Blood samples were extracted before (T0) and after (T1) a 6-week treatment, and erythrocytes were isolated for biochemical determination, phosphatidylserine externalization (EPHS), cell size and reduced glutathione (GSH) analyses, and endothelium adhesion evaluation. A reduction in glucose (4.3%) and LDL cholesterol (9.2%) was observed only in the control group, whereas in the case group, an increase in ApoA1 (6.4%) was observed. Neither EPHS, cell size nor GSH were modified by the treatment with any of the supplements, whilst endothelium adhesion was reduced (55.1%) only in the case group. These results suggest that the PS supplement may improve some cardiovascular health parameters in the target population even though eryptosis status is not modified by this treatment.https://www.mdpi.com/2304-8158/13/24/4108hypercholesterolemiaerythrocytesendothelium adhesionatherosclerosisphytosterols
spellingShingle Diego Miedes
Raquel Ortega-Luna
Sonia Broseta
Sergio Martínez-Hervás
Ángeles Álvarez-Ribelles
Víctor Collado-Díaz
Antonio Cilla
Amparo Alegría
Impact of a Plant Sterol Food Supplement on Eryptotic and Associated Cardiometabolic Parameters: A Randomized Placebo-Controlled Trial in Statin-Treated Patients
Foods
hypercholesterolemia
erythrocytes
endothelium adhesion
atherosclerosis
phytosterols
title Impact of a Plant Sterol Food Supplement on Eryptotic and Associated Cardiometabolic Parameters: A Randomized Placebo-Controlled Trial in Statin-Treated Patients
title_full Impact of a Plant Sterol Food Supplement on Eryptotic and Associated Cardiometabolic Parameters: A Randomized Placebo-Controlled Trial in Statin-Treated Patients
title_fullStr Impact of a Plant Sterol Food Supplement on Eryptotic and Associated Cardiometabolic Parameters: A Randomized Placebo-Controlled Trial in Statin-Treated Patients
title_full_unstemmed Impact of a Plant Sterol Food Supplement on Eryptotic and Associated Cardiometabolic Parameters: A Randomized Placebo-Controlled Trial in Statin-Treated Patients
title_short Impact of a Plant Sterol Food Supplement on Eryptotic and Associated Cardiometabolic Parameters: A Randomized Placebo-Controlled Trial in Statin-Treated Patients
title_sort impact of a plant sterol food supplement on eryptotic and associated cardiometabolic parameters a randomized placebo controlled trial in statin treated patients
topic hypercholesterolemia
erythrocytes
endothelium adhesion
atherosclerosis
phytosterols
url https://www.mdpi.com/2304-8158/13/24/4108
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