Protocol for conducting a single-cell sequencing assay for transposase-accessible chromatin analysis

Protocol for conducting a single-cell sequencing assay for transposase-accessible chromatin analysis

Summary: While the single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) is a powerful single-cell resolution tool for studying chromatin accessibility, its analytical workflow presents significant challenges for researchers new to scATAC-seq. Here, we present a protocol for...

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Bibliographic Details
Main Authors: Wen Ma, Peiyao Ma, Yue Yuan, Dandan Hu, Kaifeng Li, Pengfei Cai, Chuanyu Liu
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence analysis
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003661
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Summary:Summary: While the single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) is a powerful single-cell resolution tool for studying chromatin accessibility, its analytical workflow presents significant challenges for researchers new to scATAC-seq. Here, we present a protocol for conducting scATAC-seq analysis using a publicly available dataset as an example. We describe steps for data pre-processing and downstream analysis. We then detail procedures for computational multi-omics integration. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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