<i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from Somalia
Ehrlichioses, caused by <i>Ehrlichia</i> species, are tick-borne diseases (TBDs) that affect animals and humans worldwide. This study aimed to investigate the molecular occurrence of <i>Ehrlichia</i> spp. in 530 animals (155 Dromedary camels, 199 goats, 131 cattle, and 45 she...
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2025-01-01
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| author | Aamir M. Osman Ahmed A. Hassan-Kadle Marcos R. André Flávia C. M. Collere Amir Salvador Alabí Córdova Fabiano Montiani-Ferreira Thállitha S. W. J. Vieira Abdalla M. Ibrahim Abdulkarim A. Yusuf Rosangela Z. Machado Rafael F. C. Vieira |
| author_facet | Aamir M. Osman Ahmed A. Hassan-Kadle Marcos R. André Flávia C. M. Collere Amir Salvador Alabí Córdova Fabiano Montiani-Ferreira Thállitha S. W. J. Vieira Abdalla M. Ibrahim Abdulkarim A. Yusuf Rosangela Z. Machado Rafael F. C. Vieira |
| author_sort | Aamir M. Osman |
| collection | DOAJ |
| description | Ehrlichioses, caused by <i>Ehrlichia</i> species, are tick-borne diseases (TBDs) that affect animals and humans worldwide. This study aimed to investigate the molecular occurrence of <i>Ehrlichia</i> spp. in 530 animals (155 Dromedary camels, 199 goats, 131 cattle, and 45 sheep) in the Benadir and Lower Shabelle regions of Somalia. Blood DNA samples were tested for PCR targeting <i>dsb</i> and <i>sodB</i> genes of <i>Ehrlichia</i> spp. and <i>PCS20</i> and <i>map1</i> genes of <i>E. ruminantium</i>. The obtained sequences were submitted for phylogenetic analyses. <i>Ehrlichia</i> spp. were detected in 26.4% (140/530) of animals by <i>dsb</i>-PCR, with the highest prevalence in dromedary camels (54.8%), followed by cattle (29.8%), goats (7.0%), and sheep (4.4%). Dromedary camels, cattle, and goats had significantly higher infection odds compared to sheep (<i>p</i> < 0.05). Among <i>dsb</i>-PCR-positive samples, 76.9% (30/39) of cattle tested <i>sodB</i>-positive, while other species were negative. <i>E. ruminantium</i> was detected in 13.7% (18/131) of cattle by <i>pCS20</i>-PCR, but none were positive for the <i>map1</i> gene. Phylogenetic analysis confirmed <i>E. minasensis</i> in camels, sheep, and goats and <i>E. ruminantium</i> in cattle, marking the first molecular evidence of <i>E. minasensis</i> in dromedary camels, sheep, and goats globally, and <i>E. ruminantium</i> in cattle from Somalia. These findings emphasize the need for further research on its economic and public health impact. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-6de7621f1e404eb2929146c815018d572025-01-24T13:44:46ZengMDPI AGPathogens2076-08172025-01-011416510.3390/pathogens14010065<i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from SomaliaAamir M. Osman0Ahmed A. Hassan-Kadle1Marcos R. André2Flávia C. M. Collere3Amir Salvador Alabí Córdova4Fabiano Montiani-Ferreira5Thállitha S. W. J. Vieira6Abdalla M. Ibrahim7Abdulkarim A. Yusuf8Rosangela Z. Machado9Rafael F. C. Vieira10Vector-Borne Diseases Laboratory, Department of Veterinary Medicine, Universidade Federal do Paraná, Curitiba 80035-050, BrazilSomali One Health Centre, Abrar University, Mogadishu P.O. Box 25, SomaliaVector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (FCAV/UNESP), Jaboticabal CEP 14884-900, BrazilVector-Borne Diseases Laboratory, Department of Veterinary Medicine, Universidade Federal do Paraná, Curitiba 80035-050, BrazilVector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (FCAV/UNESP), Jaboticabal CEP 14884-900, BrazilVector-Borne Diseases Laboratory, Department of Veterinary Medicine, Universidade Federal do Paraná, Curitiba 80035-050, BrazilDepartment of Chemistry, The University of North Carolina at Charlotte, Charlotte, NC 28223, USASomali One Health Centre, Abrar University, Mogadishu P.O. Box 25, SomaliaAbrar Research and Training Centre, Abrar University, Mogadishu P.O. Box 25, SomaliaVector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (FCAV/UNESP), Jaboticabal CEP 14884-900, BrazilVector-Borne Diseases Laboratory, Department of Veterinary Medicine, Universidade Federal do Paraná, Curitiba 80035-050, BrazilEhrlichioses, caused by <i>Ehrlichia</i> species, are tick-borne diseases (TBDs) that affect animals and humans worldwide. This study aimed to investigate the molecular occurrence of <i>Ehrlichia</i> spp. in 530 animals (155 Dromedary camels, 199 goats, 131 cattle, and 45 sheep) in the Benadir and Lower Shabelle regions of Somalia. Blood DNA samples were tested for PCR targeting <i>dsb</i> and <i>sodB</i> genes of <i>Ehrlichia</i> spp. and <i>PCS20</i> and <i>map1</i> genes of <i>E. ruminantium</i>. The obtained sequences were submitted for phylogenetic analyses. <i>Ehrlichia</i> spp. were detected in 26.4% (140/530) of animals by <i>dsb</i>-PCR, with the highest prevalence in dromedary camels (54.8%), followed by cattle (29.8%), goats (7.0%), and sheep (4.4%). Dromedary camels, cattle, and goats had significantly higher infection odds compared to sheep (<i>p</i> < 0.05). Among <i>dsb</i>-PCR-positive samples, 76.9% (30/39) of cattle tested <i>sodB</i>-positive, while other species were negative. <i>E. ruminantium</i> was detected in 13.7% (18/131) of cattle by <i>pCS20</i>-PCR, but none were positive for the <i>map1</i> gene. Phylogenetic analysis confirmed <i>E. minasensis</i> in camels, sheep, and goats and <i>E. ruminantium</i> in cattle, marking the first molecular evidence of <i>E. minasensis</i> in dromedary camels, sheep, and goats globally, and <i>E. ruminantium</i> in cattle from Somalia. These findings emphasize the need for further research on its economic and public health impact.https://www.mdpi.com/2076-0817/14/1/65tick-borne diseasesehrlichiosis<i>Ehrlichia minasensis</i><i>Ehrlichia ruminantium</i>heartwaterSomalia |
| spellingShingle | Aamir M. Osman Ahmed A. Hassan-Kadle Marcos R. André Flávia C. M. Collere Amir Salvador Alabí Córdova Fabiano Montiani-Ferreira Thállitha S. W. J. Vieira Abdalla M. Ibrahim Abdulkarim A. Yusuf Rosangela Z. Machado Rafael F. C. Vieira <i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from Somalia Pathogens tick-borne diseases ehrlichiosis <i>Ehrlichia minasensis</i> <i>Ehrlichia ruminantium</i> heartwater Somalia |
| title | <i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from Somalia |
| title_full | <i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from Somalia |
| title_fullStr | <i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from Somalia |
| title_full_unstemmed | <i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from Somalia |
| title_short | <i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from Somalia |
| title_sort | i ehrlichia i species in dromedary camels i camelus dromedarius i and ruminants from somalia |
| topic | tick-borne diseases ehrlichiosis <i>Ehrlichia minasensis</i> <i>Ehrlichia ruminantium</i> heartwater Somalia |
| url | https://www.mdpi.com/2076-0817/14/1/65 |
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