Encapsulation of ultrasound-assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation: Physicochemical properties, release behavior under simulated gastrointestinal conditions
Flaxseed meal is rich in protein with antioxidant properties. In this study, its protein was hydrolyzed using alcalase and pancreatin (1.2 %–3 %) after ultrasonic pretreatment. The pancreatin-treated hydrolysate showed the highest antioxidant activity, with 75.66 % DPPH inhibition, 70.39 % iron ion...
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| Language: | English |
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Elsevier
2025-04-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525002366 |
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| author | Faezeh Farzanfar Alireza Sadeghi Mahoonak Mohammad Ghorbani Seyed Hossein Hosseini Ghaboos Shima Kaveh |
| author_facet | Faezeh Farzanfar Alireza Sadeghi Mahoonak Mohammad Ghorbani Seyed Hossein Hosseini Ghaboos Shima Kaveh |
| author_sort | Faezeh Farzanfar |
| collection | DOAJ |
| description | Flaxseed meal is rich in protein with antioxidant properties. In this study, its protein was hydrolyzed using alcalase and pancreatin (1.2 %–3 %) after ultrasonic pretreatment. The pancreatin-treated hydrolysate showed the highest antioxidant activity, with 75.66 % DPPH inhibition, 70.39 % iron ion chelation, and a total antioxidant activity absorption value of 0.86 nm. To enhance antioxidant stability and control release, the hydrolyzed protein was encapsulated in liposomal nanovesicles with varying flaxseed oil (0.01, 0.02 and 0.03 g/10 ml chloroform) and cholesterol (0.02, 0.03, 0.04 and 0.05 g) concentrations. Treatment L3 (0.02 g flaxseed oil) exhibited the best antioxidant and physicochemical properties, with 95.64 % encapsulation efficiency, 409 nm particle size, −15.9 mV zeta potential, 87.51 % DPPH inhibition, 56.60 % iron ion chelation, and 1.339 total antioxidant activity absorption at 695 nm. The results showed that the optimal treatment of this study (L3) can be a suitable alternative to synthetic antioxidants in food formulations. |
| format | Article |
| id | doaj-art-6da3d63349df4fa58d2674b4335b2bc6 |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-6da3d63349df4fa58d2674b4335b2bc62025-08-20T01:50:01ZengElsevierFood Chemistry: X2590-15752025-04-012710238910.1016/j.fochx.2025.102389Encapsulation of ultrasound-assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation: Physicochemical properties, release behavior under simulated gastrointestinal conditionsFaezeh Farzanfar0Alireza Sadeghi Mahoonak1Mohammad Ghorbani2Seyed Hossein Hosseini Ghaboos3Shima Kaveh4Faculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 4918943464, IranFaculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 4918943464, Iran; Corresponding author.Faculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 4918943464, IranFood Science and Technology Research Center of East Golestan, Azadshahr Branch, Islamic Azad University, Azadshahr, IranFaculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 4918943464, IranFlaxseed meal is rich in protein with antioxidant properties. In this study, its protein was hydrolyzed using alcalase and pancreatin (1.2 %–3 %) after ultrasonic pretreatment. The pancreatin-treated hydrolysate showed the highest antioxidant activity, with 75.66 % DPPH inhibition, 70.39 % iron ion chelation, and a total antioxidant activity absorption value of 0.86 nm. To enhance antioxidant stability and control release, the hydrolyzed protein was encapsulated in liposomal nanovesicles with varying flaxseed oil (0.01, 0.02 and 0.03 g/10 ml chloroform) and cholesterol (0.02, 0.03, 0.04 and 0.05 g) concentrations. Treatment L3 (0.02 g flaxseed oil) exhibited the best antioxidant and physicochemical properties, with 95.64 % encapsulation efficiency, 409 nm particle size, −15.9 mV zeta potential, 87.51 % DPPH inhibition, 56.60 % iron ion chelation, and 1.339 total antioxidant activity absorption at 695 nm. The results showed that the optimal treatment of this study (L3) can be a suitable alternative to synthetic antioxidants in food formulations.http://www.sciencedirect.com/science/article/pii/S2590157525002366FlaxseedHydrolyzed proteinNanoliposomeAntioxidantUltrasound |
| spellingShingle | Faezeh Farzanfar Alireza Sadeghi Mahoonak Mohammad Ghorbani Seyed Hossein Hosseini Ghaboos Shima Kaveh Encapsulation of ultrasound-assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation: Physicochemical properties, release behavior under simulated gastrointestinal conditions Food Chemistry: X Flaxseed Hydrolyzed protein Nanoliposome Antioxidant Ultrasound |
| title | Encapsulation of ultrasound-assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation: Physicochemical properties, release behavior under simulated gastrointestinal conditions |
| title_full | Encapsulation of ultrasound-assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation: Physicochemical properties, release behavior under simulated gastrointestinal conditions |
| title_fullStr | Encapsulation of ultrasound-assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation: Physicochemical properties, release behavior under simulated gastrointestinal conditions |
| title_full_unstemmed | Encapsulation of ultrasound-assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation: Physicochemical properties, release behavior under simulated gastrointestinal conditions |
| title_short | Encapsulation of ultrasound-assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation: Physicochemical properties, release behavior under simulated gastrointestinal conditions |
| title_sort | encapsulation of ultrasound assisted flaxseed meal protein hydrolysate in nanoliposomal systems by new formulation physicochemical properties release behavior under simulated gastrointestinal conditions |
| topic | Flaxseed Hydrolyzed protein Nanoliposome Antioxidant Ultrasound |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525002366 |
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