Viscoelastic Study of Hemostasis Disorders Associated with <i>Echis ocellatus</i> Envenoming in North Benin Using a Quantra Analyzer
<i>Echis ocellatus</i> envenomings are a public health problem in West Africa, leading to bleeding and hypocoagulability. The aim of this study was to assess the hemostasis disorders associated with <i>E. ocellatus</i> envenoming. Envenomed patients with an abnormal whole blo...
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2024-12-01
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| author | Sébastien Larréché Roland Benes Chacha Noé Sodjinou Seidou Alassane Ouorou Eric Ganhouingnon Edith Aloukoutou Layo Bruno Mégarbane Achille Massougbodji Jean-Philippe Chippaux |
| author_facet | Sébastien Larréché Roland Benes Chacha Noé Sodjinou Seidou Alassane Ouorou Eric Ganhouingnon Edith Aloukoutou Layo Bruno Mégarbane Achille Massougbodji Jean-Philippe Chippaux |
| author_sort | Sébastien Larréché |
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| description | <i>Echis ocellatus</i> envenomings are a public health problem in West Africa, leading to bleeding and hypocoagulability. The aim of this study was to assess the hemostasis disorders associated with <i>E. ocellatus</i> envenoming. Envenomed patients with an abnormal whole blood clotting test (WBCT) were prospectively included at Tanguiéta, Benin. A WBCT with a sequential reading (i.e., at 20, 30, and 60 min), viscoelastic analysis (VA) using the Quantra analyzer, and blood count were performed on admission. VA and the WBCT were also assessed at 4, 8, 12, 24, 48, and 72 h after antivenom administration. Nineteen patients were included. On admission, the main results were an absence of a clot on VA and a slight decrease in platelets. Clot time gradually decreased over time while clot stiffness, fibrinogen, and platelet contributions to stiffness increased. Sequential reading improved the sensitivity of the WBCT. At H48, all patients with recurrence bleeding after antivenom administration had an abnormal WBCT while patients with a normal WBCT never had bleeding during their follow-up. VA allows the identification of various hemostasis disorders. Hypofibrinogenemia was the main disorder that persisted for several days after treatment. A WBCT with a sequential reading is an effective alternative for monitoring hypocoagulability in the absence of a laboratory. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Toxins |
| spelling | doaj-art-6d85ab7b8239458a88e09875fa8f5a3d2025-01-24T13:51:09ZengMDPI AGToxins2072-66512024-12-01171310.3390/toxins17010003Viscoelastic Study of Hemostasis Disorders Associated with <i>Echis ocellatus</i> Envenoming in North Benin Using a Quantra AnalyzerSébastien Larréché0Roland Benes Chacha1Noé Sodjinou2Seidou Alassane Ouorou3Eric Ganhouingnon4Edith Aloukoutou Layo5Bruno Mégarbane6Achille Massougbodji7Jean-Philippe Chippaux8Department of Medical Biology, Bégin National Military Teaching Hospital, F-94160 Saint-Mandé, FranceBenin Clinical Research Institute, Abomey-Calavi, BeninBenin Clinical Research Institute, Abomey-Calavi, BeninEmergency Department, Saint-Jean de Dieu Hospital, Tanguiéta, BeninMedical Biology Laboratory, Saint-Jean de Dieu Hospital, Tanguiéta, BeninBenin Clinical Research Institute, Abomey-Calavi, BeninInserm UMRS-1144, Université Paris Cité, F-75006 Paris, FranceBenin Clinical Research Institute, Abomey-Calavi, BeninFrench National Research Institute for Development, Mother and Child in Tropical Environment: Pathogens, Health System and Epidemiological Transition, Université Paris Cité, F-75006 Paris, France<i>Echis ocellatus</i> envenomings are a public health problem in West Africa, leading to bleeding and hypocoagulability. The aim of this study was to assess the hemostasis disorders associated with <i>E. ocellatus</i> envenoming. Envenomed patients with an abnormal whole blood clotting test (WBCT) were prospectively included at Tanguiéta, Benin. A WBCT with a sequential reading (i.e., at 20, 30, and 60 min), viscoelastic analysis (VA) using the Quantra analyzer, and blood count were performed on admission. VA and the WBCT were also assessed at 4, 8, 12, 24, 48, and 72 h after antivenom administration. Nineteen patients were included. On admission, the main results were an absence of a clot on VA and a slight decrease in platelets. Clot time gradually decreased over time while clot stiffness, fibrinogen, and platelet contributions to stiffness increased. Sequential reading improved the sensitivity of the WBCT. At H48, all patients with recurrence bleeding after antivenom administration had an abnormal WBCT while patients with a normal WBCT never had bleeding during their follow-up. VA allows the identification of various hemostasis disorders. Hypofibrinogenemia was the main disorder that persisted for several days after treatment. A WBCT with a sequential reading is an effective alternative for monitoring hypocoagulability in the absence of a laboratory.https://www.mdpi.com/2072-6651/17/1/3viscoelastic assayQuantrafibrinogencoagulationfibrinolysisplatelets |
| spellingShingle | Sébastien Larréché Roland Benes Chacha Noé Sodjinou Seidou Alassane Ouorou Eric Ganhouingnon Edith Aloukoutou Layo Bruno Mégarbane Achille Massougbodji Jean-Philippe Chippaux Viscoelastic Study of Hemostasis Disorders Associated with <i>Echis ocellatus</i> Envenoming in North Benin Using a Quantra Analyzer Toxins viscoelastic assay Quantra fibrinogen coagulation fibrinolysis platelets |
| title | Viscoelastic Study of Hemostasis Disorders Associated with <i>Echis ocellatus</i> Envenoming in North Benin Using a Quantra Analyzer |
| title_full | Viscoelastic Study of Hemostasis Disorders Associated with <i>Echis ocellatus</i> Envenoming in North Benin Using a Quantra Analyzer |
| title_fullStr | Viscoelastic Study of Hemostasis Disorders Associated with <i>Echis ocellatus</i> Envenoming in North Benin Using a Quantra Analyzer |
| title_full_unstemmed | Viscoelastic Study of Hemostasis Disorders Associated with <i>Echis ocellatus</i> Envenoming in North Benin Using a Quantra Analyzer |
| title_short | Viscoelastic Study of Hemostasis Disorders Associated with <i>Echis ocellatus</i> Envenoming in North Benin Using a Quantra Analyzer |
| title_sort | viscoelastic study of hemostasis disorders associated with i echis ocellatus i envenoming in north benin using a quantra analyzer |
| topic | viscoelastic assay Quantra fibrinogen coagulation fibrinolysis platelets |
| url | https://www.mdpi.com/2072-6651/17/1/3 |
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