Dexamethasone reduces cell surface levels of CD11b on human eosinophils
Overnight incubation of human eosinophils (Eøs) with the glucocorticoid hormone dexamethasone (DEX; 0.1 μM) resulted in lower expression of the CD11b, but not CD49d, antigen on their plasma membrane, as assessed by flow cytometry. DEX produced a consistent inhibitory effect (ranging from 16% to 20%)...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1997-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359791505 |
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| _version_ | 1832561708942491648 |
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| author | A. M. Das L. H. K. Lim R. J. Flower M. Perretti |
| author_facet | A. M. Das L. H. K. Lim R. J. Flower M. Perretti |
| author_sort | A. M. Das |
| collection | DOAJ |
| description | Overnight incubation of human eosinophils (Eøs) with the glucocorticoid hormone dexamethasone (DEX; 0.1 μM) resulted in lower expression of the CD11b, but not CD49d, antigen on their plasma membrane, as assessed by flow cytometry. DEX produced a consistent inhibitory effect (ranging from 16% to 20%) when tested at a concentration of 0.1 μM. Eø stimulation with 100 ng/ml eotaxin produced an increase in CD11b (+26%), but not CD11c, levels and concomitantly a reduction (–25%) on CD62L expression. The inhibition exerted by DEX upon CD11b levels was also evident following eotaxin upregulation, with a degree of inhibition similar to that seen on basal levels. These data highlight a novel mechanism of action by which glucocorticoid hormones may be effective in reducing Eø accumulation during allergic inflammation in man. |
| format | Article |
| id | doaj-art-6d6d7f7d204c449e90721178145e9009 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6d6d7f7d204c449e90721178145e90092025-02-03T01:24:22ZengWileyMediators of Inflammation0962-93511466-18611997-01-0165-636336710.1080/09629359791505Dexamethasone reduces cell surface levels of CD11b on human eosinophilsA. M. Das0L. H. K. Lim1R. J. Flower2M. Perretti3Department of Biochemical Pharmacology, The William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, UKDepartment of Biochemical Pharmacology, The William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, UKDepartment of Biochemical Pharmacology, The William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, UKDepartment of Biochemical Pharmacology, The William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, UKOvernight incubation of human eosinophils (Eøs) with the glucocorticoid hormone dexamethasone (DEX; 0.1 μM) resulted in lower expression of the CD11b, but not CD49d, antigen on their plasma membrane, as assessed by flow cytometry. DEX produced a consistent inhibitory effect (ranging from 16% to 20%) when tested at a concentration of 0.1 μM. Eø stimulation with 100 ng/ml eotaxin produced an increase in CD11b (+26%), but not CD11c, levels and concomitantly a reduction (–25%) on CD62L expression. The inhibition exerted by DEX upon CD11b levels was also evident following eotaxin upregulation, with a degree of inhibition similar to that seen on basal levels. These data highlight a novel mechanism of action by which glucocorticoid hormones may be effective in reducing Eø accumulation during allergic inflammation in man.http://dx.doi.org/10.1080/09629359791505 |
| spellingShingle | A. M. Das L. H. K. Lim R. J. Flower M. Perretti Dexamethasone reduces cell surface levels of CD11b on human eosinophils Mediators of Inflammation |
| title | Dexamethasone reduces cell surface levels of CD11b on human eosinophils |
| title_full | Dexamethasone reduces cell surface levels of CD11b on human eosinophils |
| title_fullStr | Dexamethasone reduces cell surface levels of CD11b on human eosinophils |
| title_full_unstemmed | Dexamethasone reduces cell surface levels of CD11b on human eosinophils |
| title_short | Dexamethasone reduces cell surface levels of CD11b on human eosinophils |
| title_sort | dexamethasone reduces cell surface levels of cd11b on human eosinophils |
| url | http://dx.doi.org/10.1080/09629359791505 |
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