Genetic Markers Associated to Dyslipidemia in HIV-Infected Individuals on HAART
This study evaluated the impact of 9 single nucleotide polymorphisms (SNPs) in 6 candidate genes (APOB, APOA5, APOE, APOC3, SCAP, and LDLR) over dyslipidemia in HIV-infected patients on stable antiretroviral therapy (ART) with undetectable viral loads. Blood samples were collected from 614 patients...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/608415 |
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| author | Rosmeri K. Lazzaretti Aline S. Gasparotto Marina G. de M. Sassi Carísi A. Polanczyk Regina Kuhmmer Jussara M. Silveira Rossana P. Basso Cezar A. T. Pinheiro Mariângela F. Silveira Eduardo Sprinz Vanessa S. Mattevi |
| author_facet | Rosmeri K. Lazzaretti Aline S. Gasparotto Marina G. de M. Sassi Carísi A. Polanczyk Regina Kuhmmer Jussara M. Silveira Rossana P. Basso Cezar A. T. Pinheiro Mariângela F. Silveira Eduardo Sprinz Vanessa S. Mattevi |
| author_sort | Rosmeri K. Lazzaretti |
| collection | DOAJ |
| description | This study evaluated the impact of 9 single nucleotide polymorphisms (SNPs) in 6 candidate genes (APOB, APOA5, APOE, APOC3, SCAP, and LDLR) over dyslipidemia in HIV-infected patients on stable antiretroviral therapy (ART) with undetectable viral loads. Blood samples were collected from 614 patients at reference services in the cities of Porto Alegre, Pelotas, and Rio Grande in Brazil. The SNPs were genotyped by conventional polymerase chain reaction (PCR) and real-time PCR. The prevalence of dyslipidemia was particularly high among the protease inhibitors-treated patients (79%). APOE (rs429358 and rs7412) genotypes and APOA5 −1131T>C (rs662799) were associated with plasma triglycerides (TG) and low-density-lipoprotein cholesterol levels (LDL-C). The APOA5 −1131T>C (rs662799) and SCAP 2386A>G (rs12487736) polymorphisms were significantly associated with high-density-lipoprotein cholesterol levels. The mean values of the total cholesterol and LDL-C levels were associated with both the APOB SP Ins/Del (rs17240441) and APOB XbaI (rs693) polymorphisms. In conclusion, our data support the importance of genetic factors in the determination of lipid levels in HIV-infected individuals. Due to the relatively high number of carriers of these risk variants, studies to verify treatment implications of genotyping before HAART initiation may be advisable to guide the selection of an appropriate antiretroviral therapy regimen. |
| format | Article |
| id | doaj-art-6d5fc963e88e4fb89b8fb0d5168228ba |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-6d5fc963e88e4fb89b8fb0d5168228ba2025-02-03T05:59:29ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/608415608415Genetic Markers Associated to Dyslipidemia in HIV-Infected Individuals on HAARTRosmeri K. Lazzaretti0Aline S. Gasparotto1Marina G. de M. Sassi2Carísi A. Polanczyk3Regina Kuhmmer4Jussara M. Silveira5Rossana P. Basso6Cezar A. T. Pinheiro7Mariângela F. Silveira8Eduardo Sprinz9Vanessa S. Mattevi10Serviço de Cardiologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, 90035-903 Porto Alegre, RS, BrazilUniversidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite 245, Sala 309, 90050-170 Porto Alegre, RS, BrazilUniversidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite 245, Sala 309, 90050-170 Porto Alegre, RS, BrazilServiço de Cardiologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, 90035-903 Porto Alegre, RS, BrazilServiço de Cardiologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, 90035-903 Porto Alegre, RS, BrazilHospital Universitário Dr. Miguel Riet Correa Jr., 96200-000 Rio Grande, RS, BrazilHospital Universitário Dr. Miguel Riet Correa Jr., 96200-000 Rio Grande, RS, BrazilServiço de Assistência Especializada em HIV/AIDS, Universidade Federal de Pelotas, 96030-001 Pelotas, RS, BrazilServiço de Assistência Especializada em HIV/AIDS, Universidade Federal de Pelotas, 96030-001 Pelotas, RS, BrazilServiço de Medicina Interna, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, 90035-903 Porto Alegre, RS, BrazilUniversidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite 245, Sala 309, 90050-170 Porto Alegre, RS, BrazilThis study evaluated the impact of 9 single nucleotide polymorphisms (SNPs) in 6 candidate genes (APOB, APOA5, APOE, APOC3, SCAP, and LDLR) over dyslipidemia in HIV-infected patients on stable antiretroviral therapy (ART) with undetectable viral loads. Blood samples were collected from 614 patients at reference services in the cities of Porto Alegre, Pelotas, and Rio Grande in Brazil. The SNPs were genotyped by conventional polymerase chain reaction (PCR) and real-time PCR. The prevalence of dyslipidemia was particularly high among the protease inhibitors-treated patients (79%). APOE (rs429358 and rs7412) genotypes and APOA5 −1131T>C (rs662799) were associated with plasma triglycerides (TG) and low-density-lipoprotein cholesterol levels (LDL-C). The APOA5 −1131T>C (rs662799) and SCAP 2386A>G (rs12487736) polymorphisms were significantly associated with high-density-lipoprotein cholesterol levels. The mean values of the total cholesterol and LDL-C levels were associated with both the APOB SP Ins/Del (rs17240441) and APOB XbaI (rs693) polymorphisms. In conclusion, our data support the importance of genetic factors in the determination of lipid levels in HIV-infected individuals. Due to the relatively high number of carriers of these risk variants, studies to verify treatment implications of genotyping before HAART initiation may be advisable to guide the selection of an appropriate antiretroviral therapy regimen.http://dx.doi.org/10.1155/2013/608415 |
| spellingShingle | Rosmeri K. Lazzaretti Aline S. Gasparotto Marina G. de M. Sassi Carísi A. Polanczyk Regina Kuhmmer Jussara M. Silveira Rossana P. Basso Cezar A. T. Pinheiro Mariângela F. Silveira Eduardo Sprinz Vanessa S. Mattevi Genetic Markers Associated to Dyslipidemia in HIV-Infected Individuals on HAART The Scientific World Journal |
| title | Genetic Markers Associated to Dyslipidemia in HIV-Infected Individuals on HAART |
| title_full | Genetic Markers Associated to Dyslipidemia in HIV-Infected Individuals on HAART |
| title_fullStr | Genetic Markers Associated to Dyslipidemia in HIV-Infected Individuals on HAART |
| title_full_unstemmed | Genetic Markers Associated to Dyslipidemia in HIV-Infected Individuals on HAART |
| title_short | Genetic Markers Associated to Dyslipidemia in HIV-Infected Individuals on HAART |
| title_sort | genetic markers associated to dyslipidemia in hiv infected individuals on haart |
| url | http://dx.doi.org/10.1155/2013/608415 |
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