Robust ordered mRNA differential display: an improved method for global gene expression profiling
Global gene expression profiling (GGEP) plays a pivotal role in biological research. We developed an improved GGEP method called “robust ordered mRNA differential display (RoDD)” by combining mRNA differential display (DD) and complementary DNA amplified fragment length polymorphisms (cDNA-AFLP) usi...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2011-10-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000113752 |
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| author | Honggao Liu Jihong Hu Lei Pan Shuzhen Wang Ying He Yi Ding |
| author_facet | Honggao Liu Jihong Hu Lei Pan Shuzhen Wang Ying He Yi Ding |
| author_sort | Honggao Liu |
| collection | DOAJ |
| description | Global gene expression profiling (GGEP) plays a pivotal role in biological research. We developed an improved GGEP method called “robust ordered mRNA differential display (RoDD)” by combining mRNA differential display (DD) and complementary DNA amplified fragment length polymorphisms (cDNA-AFLP) using elaborately designed primers and a poly (dT:A) replacement technique. Redundancy was minimized by bead-based isolation and coverage was improved by using restriction enzymes that recognized 4-bp sites. This method offers the common virtues of gel-based methods along with the reliability of cDNA-AFLP. The most significant advantage of RoDD over current gel-based methods is greatly improved coverage and minimized redundancy.global gene expression profiling; mRNA differential display; complementary DNA amplified fragment length polymorphisms |
| format | Article |
| id | doaj-art-6d24d066f30242598a8fc0c0c486d7e7 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2011-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-6d24d066f30242598a8fc0c0c486d7e72025-08-20T02:25:37ZengTaylor & Francis GroupBioTechniques0736-62051940-98182011-10-0151427127510.2144/000113752Robust ordered mRNA differential display: an improved method for global gene expression profilingHonggao Liu0Jihong Hu1Lei Pan2Shuzhen Wang3Ying He4Yi Ding51State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, China1State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, China1State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, China1State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, China1State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, China1State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, ChinaGlobal gene expression profiling (GGEP) plays a pivotal role in biological research. We developed an improved GGEP method called “robust ordered mRNA differential display (RoDD)” by combining mRNA differential display (DD) and complementary DNA amplified fragment length polymorphisms (cDNA-AFLP) using elaborately designed primers and a poly (dT:A) replacement technique. Redundancy was minimized by bead-based isolation and coverage was improved by using restriction enzymes that recognized 4-bp sites. This method offers the common virtues of gel-based methods along with the reliability of cDNA-AFLP. The most significant advantage of RoDD over current gel-based methods is greatly improved coverage and minimized redundancy.global gene expression profiling; mRNA differential display; complementary DNA amplified fragment length polymorphismshttps://www.future-science.com/doi/10.2144/000113752global gene expression profilingmRNA differential displaycomplementary DNA amplified fragment length polymorphisms |
| spellingShingle | Honggao Liu Jihong Hu Lei Pan Shuzhen Wang Ying He Yi Ding Robust ordered mRNA differential display: an improved method for global gene expression profiling BioTechniques global gene expression profiling mRNA differential display complementary DNA amplified fragment length polymorphisms |
| title | Robust ordered mRNA differential display: an improved method for global gene expression profiling |
| title_full | Robust ordered mRNA differential display: an improved method for global gene expression profiling |
| title_fullStr | Robust ordered mRNA differential display: an improved method for global gene expression profiling |
| title_full_unstemmed | Robust ordered mRNA differential display: an improved method for global gene expression profiling |
| title_short | Robust ordered mRNA differential display: an improved method for global gene expression profiling |
| title_sort | robust ordered mrna differential display an improved method for global gene expression profiling |
| topic | global gene expression profiling mRNA differential display complementary DNA amplified fragment length polymorphisms |
| url | https://www.future-science.com/doi/10.2144/000113752 |
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