Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
Macrophages play an important role in human immunodeficiency virus (HIV) pathogenesis and contribute to establishment of a viral reservoir responsible for continuous virus production and virus transmission to T cells. In this study, we investigated the differences between various monocyte-derived ma...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/208412 |
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| author | Elisabeth A. Diget Kaja Zuwala Randi K. Berg Rune R. Laursen Stine Søby Lars Østergaard Jesper Melchjorsen Trine H. Mogensen |
| author_facet | Elisabeth A. Diget Kaja Zuwala Randi K. Berg Rune R. Laursen Stine Søby Lars Østergaard Jesper Melchjorsen Trine H. Mogensen |
| author_sort | Elisabeth A. Diget |
| collection | DOAJ |
| description | Macrophages play an important role in human immunodeficiency virus (HIV) pathogenesis and contribute to establishment of a viral reservoir responsible for continuous virus production and virus transmission to T cells. In this study, we investigated the differences between various monocyte-derived macrophages (MDMs) generated through different differentiation protocols and evaluated different cellular, immunological, and virological properties. We found that elevated and persistent HIV-1 pWT/BaL replication could be obtained only in MDMs grown in RPMI containing macrophage colony-stimulating factor (M-CSF). Interestingly, this MDM type was also most responsive to toll-like receptor stimulation. By contrast, all MDM types were activated to a comparable extent by intracellular DNA, and the macrophage serum-free medium-(Mac-SFM-)differentiated MDMs responded strongly to membrane fusion through expression of CXCL10. Finally, we found that HIV infection of RPMI/M-CSF-differentiated MDMs induced low-grade expression of two interferon-stimulated genes in some donors. In conclusion, our study demonstrates that the differentiation protocol used greatly influences the ability of MDMs to activate innate immune reactions and support HIV-1 replication. Paradoxically, the data show that the MDMs with the strongest innate immune response were also the most permissive for HIV-1 replication. |
| format | Article |
| id | doaj-art-6d1d2d9709754c578161dd7559a7f417 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6d1d2d9709754c578161dd7559a7f4172025-02-03T06:12:22ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/208412208412Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived MacrophagesElisabeth A. Diget0Kaja Zuwala1Randi K. Berg2Rune R. Laursen3Stine Søby4Lars Østergaard5Jesper Melchjorsen6Trine H. Mogensen7Department of Infectious Diseases, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkDepartment of Infectious Diseases, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkDepartment of Infectious Diseases, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkDepartment of Infectious Diseases, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkDepartment of Infectious Diseases, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkDepartment of Infectious Diseases, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkDepartment of Infectious Diseases, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkDepartment of Infectious Diseases, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkMacrophages play an important role in human immunodeficiency virus (HIV) pathogenesis and contribute to establishment of a viral reservoir responsible for continuous virus production and virus transmission to T cells. In this study, we investigated the differences between various monocyte-derived macrophages (MDMs) generated through different differentiation protocols and evaluated different cellular, immunological, and virological properties. We found that elevated and persistent HIV-1 pWT/BaL replication could be obtained only in MDMs grown in RPMI containing macrophage colony-stimulating factor (M-CSF). Interestingly, this MDM type was also most responsive to toll-like receptor stimulation. By contrast, all MDM types were activated to a comparable extent by intracellular DNA, and the macrophage serum-free medium-(Mac-SFM-)differentiated MDMs responded strongly to membrane fusion through expression of CXCL10. Finally, we found that HIV infection of RPMI/M-CSF-differentiated MDMs induced low-grade expression of two interferon-stimulated genes in some donors. In conclusion, our study demonstrates that the differentiation protocol used greatly influences the ability of MDMs to activate innate immune reactions and support HIV-1 replication. Paradoxically, the data show that the MDMs with the strongest innate immune response were also the most permissive for HIV-1 replication.http://dx.doi.org/10.1155/2013/208412 |
| spellingShingle | Elisabeth A. Diget Kaja Zuwala Randi K. Berg Rune R. Laursen Stine Søby Lars Østergaard Jesper Melchjorsen Trine H. Mogensen Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages Mediators of Inflammation |
| title | Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages |
| title_full | Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages |
| title_fullStr | Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages |
| title_full_unstemmed | Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages |
| title_short | Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages |
| title_sort | characterization of hiv 1 infection and innate sensing in different types of primary human monocyte derived macrophages |
| url | http://dx.doi.org/10.1155/2013/208412 |
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