High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing <i>Escherichia coli</i> ST69
<b>Background/Objectives:</b> In 2022, an outbreak of H5N1 highly pathogenic avian influenza (HPAI) killed 60% of the largest breeding colony of Dalmatian pelicans (DPs) in the world at Mikri Prespa Lake (Greece), prompting a multidisciplinary study on HPAI and other pathogens. This stud...
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2025-01-01
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| author | Teresa Cardona-Cabrera Sandra Martínez-Álvarez Carmen González-Azcona Carlos Javier Gijón-García Olga Alexandrou Giorgos Catsadorakis Panagiotis Azmanis Carmen Torres Ursula Höfle |
| author_facet | Teresa Cardona-Cabrera Sandra Martínez-Álvarez Carmen González-Azcona Carlos Javier Gijón-García Olga Alexandrou Giorgos Catsadorakis Panagiotis Azmanis Carmen Torres Ursula Höfle |
| author_sort | Teresa Cardona-Cabrera |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> In 2022, an outbreak of H5N1 highly pathogenic avian influenza (HPAI) killed 60% of the largest breeding colony of Dalmatian pelicans (DPs) in the world at Mikri Prespa Lake (Greece), prompting a multidisciplinary study on HPAI and other pathogens. This study determines the antimicrobial resistance rates of cloacal enterococci and <i>Escherichia coli</i> in DPs. <b>Methods:</b> Fifty-two blood and cloacal swab samples were collected from 31 nestlings (20 DP/11 great white pelicans) hatched after the H5N1 outbreak at the Prespa colony and 21 subadult/adult DPs captured at a spring migration stopover. The swabs were inoculated in non-selective and chromogenic-selective media. Identification was performed using MALDI-TOF, and antimicrobial susceptibility was tested. The genetic content was characterized using PCR and sequencing, and the clonality of extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> isolates was characterized using Multilocus Sequence Typing. <b>Results:</b> Twenty-eight non-repetitive <i>E. coli</i> and 45 enterococci isolates were recovered in non-selective media; most of them were susceptible to all antibiotics tested (85.7% <i>E. coli</i>/91.1% enterococci). Three of the fifty-two samples (6%, all adults) contained ESBL-<i>E. coli</i> isolates (detected in chromogenic ESBL plates), all carrying the <i>bla</i><sub>CTX-M-15</sub> gene and belonging to the lineage ST69. <b>Conclusions:</b> Despite the susceptibility of most fecal <i>E. coli</i> and enterococci isolates to all antibiotics tested, the finding that <i>E. coli</i> of lineage ST69 carry <i>bla</i><sub>CTX-M-15</sub> is of concern. This high-risk clone needs further investigation to elucidate its primary sources and address the growing threat of antimicrobial resistance from an integrated “One Health” perspective. Furthermore, it is imperative to study the potential impacts of ESBL-<i>E. coli</i> on the endangered DP further. |
| format | Article |
| id | doaj-art-6d162ee2f5b343c1bd6c64ffc9d32282 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-6d162ee2f5b343c1bd6c64ffc9d322822025-01-24T13:18:54ZengMDPI AGAntibiotics2079-63822025-01-011418310.3390/antibiotics14010083High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing <i>Escherichia coli</i> ST69Teresa Cardona-Cabrera0Sandra Martínez-Álvarez1Carmen González-Azcona2Carlos Javier Gijón-García3Olga Alexandrou4Giorgos Catsadorakis5Panagiotis Azmanis6Carmen Torres7Ursula Höfle8SaBio Health and Biotechnology Research Group, Institute for Game and Wildlife Research (IREC), Ronda de Toledo 12, 13071 Ciudad Real, SpainArea of Biochemistry and Molecular Biology, One Health-UR Research Group, University of La Rioja, Calle Madre de Dios 53, 26006 Logroño, SpainArea of Biochemistry and Molecular Biology, One Health-UR Research Group, University of La Rioja, Calle Madre de Dios 53, 26006 Logroño, SpainSaBio Health and Biotechnology Research Group, Institute for Game and Wildlife Research (IREC), Ronda de Toledo 12, 13071 Ciudad Real, SpainSociety for the Protection of Prespa, Agios Germanos, Prespa, 53150 Florina, GreeceSociety for the Protection of Prespa, Agios Germanos, Prespa, 53150 Florina, GreeceDubai Falcon Hospital, 22a Street, Zabeel 2, Dubai P.O. Box 23919, United Arab EmiratesArea of Biochemistry and Molecular Biology, One Health-UR Research Group, University of La Rioja, Calle Madre de Dios 53, 26006 Logroño, SpainSaBio Health and Biotechnology Research Group, Institute for Game and Wildlife Research (IREC), Ronda de Toledo 12, 13071 Ciudad Real, Spain<b>Background/Objectives:</b> In 2022, an outbreak of H5N1 highly pathogenic avian influenza (HPAI) killed 60% of the largest breeding colony of Dalmatian pelicans (DPs) in the world at Mikri Prespa Lake (Greece), prompting a multidisciplinary study on HPAI and other pathogens. This study determines the antimicrobial resistance rates of cloacal enterococci and <i>Escherichia coli</i> in DPs. <b>Methods:</b> Fifty-two blood and cloacal swab samples were collected from 31 nestlings (20 DP/11 great white pelicans) hatched after the H5N1 outbreak at the Prespa colony and 21 subadult/adult DPs captured at a spring migration stopover. The swabs were inoculated in non-selective and chromogenic-selective media. Identification was performed using MALDI-TOF, and antimicrobial susceptibility was tested. The genetic content was characterized using PCR and sequencing, and the clonality of extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> isolates was characterized using Multilocus Sequence Typing. <b>Results:</b> Twenty-eight non-repetitive <i>E. coli</i> and 45 enterococci isolates were recovered in non-selective media; most of them were susceptible to all antibiotics tested (85.7% <i>E. coli</i>/91.1% enterococci). Three of the fifty-two samples (6%, all adults) contained ESBL-<i>E. coli</i> isolates (detected in chromogenic ESBL plates), all carrying the <i>bla</i><sub>CTX-M-15</sub> gene and belonging to the lineage ST69. <b>Conclusions:</b> Despite the susceptibility of most fecal <i>E. coli</i> and enterococci isolates to all antibiotics tested, the finding that <i>E. coli</i> of lineage ST69 carry <i>bla</i><sub>CTX-M-15</sub> is of concern. This high-risk clone needs further investigation to elucidate its primary sources and address the growing threat of antimicrobial resistance from an integrated “One Health” perspective. Furthermore, it is imperative to study the potential impacts of ESBL-<i>E. coli</i> on the endangered DP further.https://www.mdpi.com/2079-6382/14/1/83Dalmatian pelicanantimicrobial resistanceESBL-<i>E. coli</i>One Health |
| spellingShingle | Teresa Cardona-Cabrera Sandra Martínez-Álvarez Carmen González-Azcona Carlos Javier Gijón-García Olga Alexandrou Giorgos Catsadorakis Panagiotis Azmanis Carmen Torres Ursula Höfle High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing <i>Escherichia coli</i> ST69 Antibiotics Dalmatian pelican antimicrobial resistance ESBL-<i>E. coli</i> One Health |
| title | High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing <i>Escherichia coli</i> ST69 |
| title_full | High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing <i>Escherichia coli</i> ST69 |
| title_fullStr | High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing <i>Escherichia coli</i> ST69 |
| title_full_unstemmed | High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing <i>Escherichia coli</i> ST69 |
| title_short | High Antimicrobial Susceptibility of Cloacal Enterococci and <i>Escherichia coli</i> from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing <i>Escherichia coli</i> ST69 |
| title_sort | high antimicrobial susceptibility of cloacal enterococci and i escherichia coli i from free living dalmatian and great white pelicans with detection of cefotaximase ctx m 15 producing i escherichia coli i st69 |
| topic | Dalmatian pelican antimicrobial resistance ESBL-<i>E. coli</i> One Health |
| url | https://www.mdpi.com/2079-6382/14/1/83 |
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