Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A
In human skeletal muscle, myoblasts represent the main population of myogenic progenitors. We previously showed that, beside their myogenic differentiation capacities, myoblasts also differentiate towards osteogenic and chondrogenic lineages, some properties generally considered being hallmarks of m...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/412610 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548607762366464 |
|---|---|
| author | Séverine Lecourt Yves Lepelletier Valérie Vanneaux Rafika Jarray Thomas Domet Françoise Raynaud Réda Hadj-Slimane Audrey Cras Olivier Hermine Jean-Pierre Marolleau Jérôme Larghero |
| author_facet | Séverine Lecourt Yves Lepelletier Valérie Vanneaux Rafika Jarray Thomas Domet Françoise Raynaud Réda Hadj-Slimane Audrey Cras Olivier Hermine Jean-Pierre Marolleau Jérôme Larghero |
| author_sort | Séverine Lecourt |
| collection | DOAJ |
| description | In human skeletal muscle, myoblasts represent the main population of myogenic progenitors. We previously showed that, beside their myogenic differentiation capacities, myoblasts also differentiate towards osteogenic and chondrogenic lineages, some properties generally considered being hallmarks of mesenchymal stem cells (MSCs). MSCs are also characterized by their immunosuppressive potential, through cell-cell contacts and soluble factors, including prostaglandin E-2 (PGE-2), transforming growth factor-β1 (TGF-β1), interleukine-10, or indoleamine 2,3-dioxygenase. We and others also reported that Galectin-1 (Gal-1) and Semaphorin-3A (Sema-3A) were involved in MSCs-mediated immunosuppression. Here, we show that human myoblasts induce a significant and dose-dependant proliferation inhibition, independently of PGE-2 and TGF-β1. Our experiments revealed that myoblasts, in culture or in situ in human muscles, expressed and secreted Gal-1 and Sema-3A. Furthermore, myoblasts immunosuppressive functions were reverted by using blocking antibodies against Gal-1 or Sema-3A. Together, these results demonstrate an unsuspected immunosuppressive effect of myoblasts that may open new therapeutic perspectives. |
| format | Article |
| id | doaj-art-6ccbb356d99d4647a6598754cef9bfbe |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6ccbb356d99d4647a6598754cef9bfbe2025-02-03T06:13:38ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/412610412610Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3ASéverine Lecourt0Yves Lepelletier1Valérie Vanneaux2Rafika Jarray3Thomas Domet4Françoise Raynaud5Réda Hadj-Slimane6Audrey Cras7Olivier Hermine8Jean-Pierre Marolleau9Jérôme Larghero10INSERM UMR940, Institut Universitaire d'Hématologie, 75475 Paris Cedex 10, FranceCNRS UMR 8147, Hôpital Necker and Université Paris Descartes, 75743 Paris Cedex 15, FranceINSERM UMR940, Institut Universitaire d'Hématologie, 75475 Paris Cedex 10, FranceLaboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, CNRS UMR 8601, Université Paris Descartes, 75006 Paris, FranceUnité de Thérapie Cellulaire et CIC de Biothérapies, Hôpital Saint Louis, AP-HP, 75475 Paris Cedex 10, FranceLaboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, CNRS UMR 8601, Université Paris Descartes, 75006 Paris, FranceTRAGEX PHARMA, 75015 Paris, FranceINSERM UMR940, Institut Universitaire d'Hématologie, 75475 Paris Cedex 10, FranceCNRS UMR 8147, Hôpital Necker and Université Paris Descartes, 75743 Paris Cedex 15, FranceService d'Hématologie Clinique, Hôpital d'Amiens, 80054 Amiens Cedex 1, FranceINSERM UMR940, Institut Universitaire d'Hématologie, 75475 Paris Cedex 10, FranceIn human skeletal muscle, myoblasts represent the main population of myogenic progenitors. We previously showed that, beside their myogenic differentiation capacities, myoblasts also differentiate towards osteogenic and chondrogenic lineages, some properties generally considered being hallmarks of mesenchymal stem cells (MSCs). MSCs are also characterized by their immunosuppressive potential, through cell-cell contacts and soluble factors, including prostaglandin E-2 (PGE-2), transforming growth factor-β1 (TGF-β1), interleukine-10, or indoleamine 2,3-dioxygenase. We and others also reported that Galectin-1 (Gal-1) and Semaphorin-3A (Sema-3A) were involved in MSCs-mediated immunosuppression. Here, we show that human myoblasts induce a significant and dose-dependant proliferation inhibition, independently of PGE-2 and TGF-β1. Our experiments revealed that myoblasts, in culture or in situ in human muscles, expressed and secreted Gal-1 and Sema-3A. Furthermore, myoblasts immunosuppressive functions were reverted by using blocking antibodies against Gal-1 or Sema-3A. Together, these results demonstrate an unsuspected immunosuppressive effect of myoblasts that may open new therapeutic perspectives.http://dx.doi.org/10.1155/2012/412610 |
| spellingShingle | Séverine Lecourt Yves Lepelletier Valérie Vanneaux Rafika Jarray Thomas Domet Françoise Raynaud Réda Hadj-Slimane Audrey Cras Olivier Hermine Jean-Pierre Marolleau Jérôme Larghero Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A Stem Cells International |
| title | Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A |
| title_full | Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A |
| title_fullStr | Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A |
| title_full_unstemmed | Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A |
| title_short | Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A |
| title_sort | human muscle progenitor cells displayed immunosuppressive effect through galectin 1 and semaphorin 3a |
| url | http://dx.doi.org/10.1155/2012/412610 |
| work_keys_str_mv | AT severinelecourt humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT yveslepelletier humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT valerievanneaux humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT rafikajarray humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT thomasdomet humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT francoiseraynaud humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT redahadjslimane humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT audreycras humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT olivierhermine humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT jeanpierremarolleau humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a AT jeromelarghero humanmuscleprogenitorcellsdisplayedimmunosuppressiveeffectthroughgalectin1andsemaphorin3a |