Optimizing the production of Polyphosphate from Acinetobacter towneri
Inorganic polyphosphates (PolyP) are linear polymers of few to several hundred orthophosphate residues, linked by energy-rich phosphoanhydride bonds. Four isolates had been screened from soil sample. By MALDI-TOF analysis, they were identified as Bacillius cereus, Acinetobacter towneri, B. megateriu...
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2015-01-01
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| Series: | Global Journal of Environmental Science and Management |
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| author | J. Aravind T. Saranya P. Kanmani |
| author_facet | J. Aravind T. Saranya P. Kanmani |
| author_sort | J. Aravind |
| collection | DOAJ |
| description | Inorganic polyphosphates (PolyP) are linear polymers of few to several hundred orthophosphate residues, linked by energy-rich phosphoanhydride bonds. Four isolates had been screened from soil sample. By MALDI-TOF analysis, they were identified as Bacillius cereus, Acinetobacter towneri, B. megaterium and B. cereus. The production of PolyP in four isolates was studied in phosphate uptake medium and sulfur deficient medium at pH 7. These organisms had shown significant production of PolyP after 22h of incubation. PolyP was extracted from the cells using alkaline lysis method. Among those isolates, Acinetobacter towneri was found to have high (24.57% w/w as P) accumulation of PolyP in sulfur deficient medium. The media optimization for sulfur deficiency was carried out using Response surface methodology (RSM). It was proven that increase in phosphate level in the presence of glucose, under sulfur limiting condition, enhanced the phosphate accumulation by Acinetobacter towneri and these condition can be simulated for the effective removal of phosphate from wastewater sources. |
| format | Article |
| id | doaj-art-6cada71dd2df446292b0acc1c13f9f6e |
| institution | Kabale University |
| issn | 2383-3572 2383-3866 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | GJESM Publisher |
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| series | Global Journal of Environmental Science and Management |
| spelling | doaj-art-6cada71dd2df446292b0acc1c13f9f6e2025-02-02T16:15:53ZengGJESM PublisherGlobal Journal of Environmental Science and Management2383-35722383-38662015-01-011163709607Optimizing the production of Polyphosphate from Acinetobacter towneriJ. Aravind0T. Saranya1P. Kanmani2Department of Biotechnology, Kumaraguru College of Technology, Coimbatore – 641006, IndiaDepartment of Biotechnology, Kumaraguru College of Technology, Coimbatore – 641006, IndiaDepartment of Biotechnology, Kumaraguru College of Technology, Coimbatore – 641006, IndiaInorganic polyphosphates (PolyP) are linear polymers of few to several hundred orthophosphate residues, linked by energy-rich phosphoanhydride bonds. Four isolates had been screened from soil sample. By MALDI-TOF analysis, they were identified as Bacillius cereus, Acinetobacter towneri, B. megaterium and B. cereus. The production of PolyP in four isolates was studied in phosphate uptake medium and sulfur deficient medium at pH 7. These organisms had shown significant production of PolyP after 22h of incubation. PolyP was extracted from the cells using alkaline lysis method. Among those isolates, Acinetobacter towneri was found to have high (24.57% w/w as P) accumulation of PolyP in sulfur deficient medium. The media optimization for sulfur deficiency was carried out using Response surface methodology (RSM). It was proven that increase in phosphate level in the presence of glucose, under sulfur limiting condition, enhanced the phosphate accumulation by Acinetobacter towneri and these condition can be simulated for the effective removal of phosphate from wastewater sources.http://www.gjesm.net/pdf_9607_bd53d82dabce789c407f9b3874648a64.htmlBiopolymerMALDI-TOFNeisser stainPlackett-BurmanPolyphosphatesSulfur deficient medium |
| spellingShingle | J. Aravind T. Saranya P. Kanmani Optimizing the production of Polyphosphate from Acinetobacter towneri Global Journal of Environmental Science and Management Biopolymer MALDI-TOF Neisser stain Plackett-Burman Polyphosphates Sulfur deficient medium |
| title | Optimizing the production of Polyphosphate from Acinetobacter towneri |
| title_full | Optimizing the production of Polyphosphate from Acinetobacter towneri |
| title_fullStr | Optimizing the production of Polyphosphate from Acinetobacter towneri |
| title_full_unstemmed | Optimizing the production of Polyphosphate from Acinetobacter towneri |
| title_short | Optimizing the production of Polyphosphate from Acinetobacter towneri |
| title_sort | optimizing the production of polyphosphate from acinetobacter towneri |
| topic | Biopolymer MALDI-TOF Neisser stain Plackett-Burman Polyphosphates Sulfur deficient medium |
| url | http://www.gjesm.net/pdf_9607_bd53d82dabce789c407f9b3874648a64.html |
| work_keys_str_mv | AT jaravind optimizingtheproductionofpolyphosphatefromacinetobactertowneri AT tsaranya optimizingtheproductionofpolyphosphatefromacinetobactertowneri AT pkanmani optimizingtheproductionofpolyphosphatefromacinetobactertowneri |