Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources
The spontaneous expression of neural markers, already demonstrated in bone marrow (BM) mesenchymal stem cells (MSCs), has been considered as evidence of the MSCs’ predisposition to differentiate toward neural lineages, supporting their use in stem cell-based therapy for neural repair. In this study...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/987678 |
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| author | Dana Foudah Marianna Monfrini Elisabetta Donzelli Stefania Niada Anna T. Brini Monia Orciani Giovanni Tredici Mariarosaria Miloso |
| author_facet | Dana Foudah Marianna Monfrini Elisabetta Donzelli Stefania Niada Anna T. Brini Monia Orciani Giovanni Tredici Mariarosaria Miloso |
| author_sort | Dana Foudah |
| collection | DOAJ |
| description | The spontaneous expression of neural markers, already demonstrated in bone marrow (BM) mesenchymal stem cells (MSCs), has been considered as evidence of the MSCs’ predisposition to differentiate toward neural lineages, supporting their use in stem cell-based therapy for neural repair. In this study we have evaluated, by immunocytochemistry, immunoblotting, and flow cytometry experiments, the expression of neural markers in undifferentiated MSCs from different sources: human adipose stem cells (hASCs), human skin-derived mesenchymal stem cells (hS-MSCs), human periodontal ligament stem cells (hPDLSCs,) and human dental pulp stem cells (hDPSCs). Our results demonstrate that the neuronal markers βIII-tubulin and NeuN, unlike other evaluated markers, are spontaneously expressed by a very high percentage of undifferentiated hASCs, hS-MSCs, hPDLSCs, and hDPSCs. Conversely, the neural progenitor marker nestin is expressed only by a high percentage of undifferentiated hPDLSCs and hDPSCs. Our results suggest that the expression of βIII-tubulin and NeuN could be a common feature of stem cells and not exclusive to neuronal cells. This could result in a reassessment of the use of βIII-tubulin and NeuN as the only evidence proving neuronal differentiation. Further studies will be necessary to elucidate the relevance of the spontaneous expression of these markers in stem cells. |
| format | Article |
| id | doaj-art-6ca1f5badb77444194de27403c521be1 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6ca1f5badb77444194de27403c521be12025-02-03T01:02:35ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/987678987678Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different SourcesDana Foudah0Marianna Monfrini1Elisabetta Donzelli2Stefania Niada3Anna T. Brini4Monia Orciani5Giovanni Tredici6Mariarosaria Miloso7Department of Surgery and Translational Medicine, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Surgery and Translational Medicine, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Surgery and Translational Medicine, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, 20129 Milano, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, 20129 Milano, ItalyDepartment of Clinical and Molecular Sciences—Histology, University Politecnica delle Marche, 60126 Ancona, ItalyDepartment of Surgery and Translational Medicine, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Surgery and Translational Medicine, University of Milano-Bicocca, 20900 Monza, ItalyThe spontaneous expression of neural markers, already demonstrated in bone marrow (BM) mesenchymal stem cells (MSCs), has been considered as evidence of the MSCs’ predisposition to differentiate toward neural lineages, supporting their use in stem cell-based therapy for neural repair. In this study we have evaluated, by immunocytochemistry, immunoblotting, and flow cytometry experiments, the expression of neural markers in undifferentiated MSCs from different sources: human adipose stem cells (hASCs), human skin-derived mesenchymal stem cells (hS-MSCs), human periodontal ligament stem cells (hPDLSCs,) and human dental pulp stem cells (hDPSCs). Our results demonstrate that the neuronal markers βIII-tubulin and NeuN, unlike other evaluated markers, are spontaneously expressed by a very high percentage of undifferentiated hASCs, hS-MSCs, hPDLSCs, and hDPSCs. Conversely, the neural progenitor marker nestin is expressed only by a high percentage of undifferentiated hPDLSCs and hDPSCs. Our results suggest that the expression of βIII-tubulin and NeuN could be a common feature of stem cells and not exclusive to neuronal cells. This could result in a reassessment of the use of βIII-tubulin and NeuN as the only evidence proving neuronal differentiation. Further studies will be necessary to elucidate the relevance of the spontaneous expression of these markers in stem cells.http://dx.doi.org/10.1155/2014/987678 |
| spellingShingle | Dana Foudah Marianna Monfrini Elisabetta Donzelli Stefania Niada Anna T. Brini Monia Orciani Giovanni Tredici Mariarosaria Miloso Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources Journal of Immunology Research |
| title | Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources |
| title_full | Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources |
| title_fullStr | Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources |
| title_full_unstemmed | Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources |
| title_short | Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources |
| title_sort | expression of neural markers by undifferentiated mesenchymal like stem cells from different sources |
| url | http://dx.doi.org/10.1155/2014/987678 |
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