Purification and Characterization of a New Lectin from Loach Skin Mucus
Lectin from loach skin mucus plays an important role in pathogen defense. However, hardly can any paper relevant to the character of lectin from loach skin mucus be found in recent years. In this study, a kind of new lectin (LML), with a high hemagglutination activity of 166.23 × 103 HU/mg, was succ...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/3853646 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548767621971968 |
|---|---|
| author | Peng-peng Sun Yuan-yuan Ren Jie Zheng Ai-jun Hu |
| author_facet | Peng-peng Sun Yuan-yuan Ren Jie Zheng Ai-jun Hu |
| author_sort | Peng-peng Sun |
| collection | DOAJ |
| description | Lectin from loach skin mucus plays an important role in pathogen defense. However, hardly can any paper relevant to the character of lectin from loach skin mucus be found in recent years. In this study, a kind of new lectin (LML), with a high hemagglutination activity of 166.23 × 103 HU/mg, was successfully isolated and purified from loach skin mucus. LML was a kind of glycoprotein with a molecular weight of 245 kDa. Also, the monosaccharide composition suggested that its carbohydrate chain was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 2.02 : 11.66 : 2.06 : 1.00 : 14.09 : 6.00. Besides, LML depended on Ca2+ to induce hemagglutination and was strongly inhibited by D-lactose. The lectin exhibited powerful resistance to alkali and kept about 30% hemagglutination activity at pH 14.0, whereas its capacity of acid resistance was weak. The maximum hemagglutination activity of LML maintained at a temperature range from 20°C to 50°C. Moreover, the structure of LML was preliminarily studied, indicating it contained abundant glutamic acid, histidine, and serine, and its secondary structure contained α-helix (4.97%), β-sheet (27.55%), turns structure (49.78%), and unordered structure (17.70%). |
| format | Article |
| id | doaj-art-6c9ea3891cf14be6a0eedd200cbd2632 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-6c9ea3891cf14be6a0eedd200cbd26322025-02-03T06:13:11ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/38536463853646Purification and Characterization of a New Lectin from Loach Skin MucusPeng-peng Sun0Yuan-yuan Ren1Jie Zheng2Ai-jun Hu3State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaLectin from loach skin mucus plays an important role in pathogen defense. However, hardly can any paper relevant to the character of lectin from loach skin mucus be found in recent years. In this study, a kind of new lectin (LML), with a high hemagglutination activity of 166.23 × 103 HU/mg, was successfully isolated and purified from loach skin mucus. LML was a kind of glycoprotein with a molecular weight of 245 kDa. Also, the monosaccharide composition suggested that its carbohydrate chain was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 2.02 : 11.66 : 2.06 : 1.00 : 14.09 : 6.00. Besides, LML depended on Ca2+ to induce hemagglutination and was strongly inhibited by D-lactose. The lectin exhibited powerful resistance to alkali and kept about 30% hemagglutination activity at pH 14.0, whereas its capacity of acid resistance was weak. The maximum hemagglutination activity of LML maintained at a temperature range from 20°C to 50°C. Moreover, the structure of LML was preliminarily studied, indicating it contained abundant glutamic acid, histidine, and serine, and its secondary structure contained α-helix (4.97%), β-sheet (27.55%), turns structure (49.78%), and unordered structure (17.70%).http://dx.doi.org/10.1155/2019/3853646 |
| spellingShingle | Peng-peng Sun Yuan-yuan Ren Jie Zheng Ai-jun Hu Purification and Characterization of a New Lectin from Loach Skin Mucus Journal of Chemistry |
| title | Purification and Characterization of a New Lectin from Loach Skin Mucus |
| title_full | Purification and Characterization of a New Lectin from Loach Skin Mucus |
| title_fullStr | Purification and Characterization of a New Lectin from Loach Skin Mucus |
| title_full_unstemmed | Purification and Characterization of a New Lectin from Loach Skin Mucus |
| title_short | Purification and Characterization of a New Lectin from Loach Skin Mucus |
| title_sort | purification and characterization of a new lectin from loach skin mucus |
| url | http://dx.doi.org/10.1155/2019/3853646 |
| work_keys_str_mv | AT pengpengsun purificationandcharacterizationofanewlectinfromloachskinmucus AT yuanyuanren purificationandcharacterizationofanewlectinfromloachskinmucus AT jiezheng purificationandcharacterizationofanewlectinfromloachskinmucus AT aijunhu purificationandcharacterizationofanewlectinfromloachskinmucus |