Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats
Objective. To investigate the effects of Dendrobium officinale polysaccharides (DOPS) on the expression of inflammatory factors IL-1β and TNF-α and the MKP-1/MAPK signal pathway. Methods. PTZ-induced epileptic rat models were established. The rats were randomly divided into four groups: the control...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2019/9058161 |
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| _version_ | 1832548677071142912 |
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| author | Lin Zhang Hua Peng Jin Xu Yixin Xu You Yin Bin He Jianhua Zhuang |
| author_facet | Lin Zhang Hua Peng Jin Xu Yixin Xu You Yin Bin He Jianhua Zhuang |
| author_sort | Lin Zhang |
| collection | DOAJ |
| description | Objective. To investigate the effects of Dendrobium officinale polysaccharides (DOPS) on the expression of inflammatory factors IL-1β and TNF-α and the MKP-1/MAPK signal pathway. Methods. PTZ-induced epileptic rat models were established. The rats were randomly divided into four groups: the control group, the DOPS group, the model group, and the DOPS intervention group. RT-PCR was used to measure the mRNA expression of IL-1β and TNF-α in the hippocampi of all groups; western blot was used to measure the protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, p38, and MKP-1 in the hippocampi of all groups at weeks 1, 2, 3, and 4 after modeling. Results. At weeks 1, 2, 3, and 4 after modeling, there were no significant differences between the control group and the DOPS group in the mRNA and protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, p38, and MKP-1 (all P>0.05); the mRNA and protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, and p38 were significantly increased, while the phosphorylation of MKP-1 was decreased in the model group compared with the control group. The mRNA and protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, and p38 were significantly decreased, while the phosphorylation of MKP-1 was increased in the DOPS intervention group compared with the model group. Conclusion. DOPS can reduce PTZ-induced brain inflammation and seizures of epileptic rats by inhibiting IL-1β, TNF-α, and MAPK signal pathways. |
| format | Article |
| id | doaj-art-6c913fcdf4814df3b3cb9db1f54c2187 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-6c913fcdf4814df3b3cb9db1f54c21872025-02-03T06:13:22ZengWileyInternational Journal of Polymer Science1687-94221687-94302019-01-01201910.1155/2019/90581619058161Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic RatsLin Zhang0Hua Peng1Jin Xu2Yixin Xu3You Yin4Bin He5Jianhua Zhuang6Department of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, ChinaDepartment of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, ChinaDepartment of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, ChinaDepartment of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, ChinaDepartment of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, ChinaDepartment of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, ChinaDepartment of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, ChinaObjective. To investigate the effects of Dendrobium officinale polysaccharides (DOPS) on the expression of inflammatory factors IL-1β and TNF-α and the MKP-1/MAPK signal pathway. Methods. PTZ-induced epileptic rat models were established. The rats were randomly divided into four groups: the control group, the DOPS group, the model group, and the DOPS intervention group. RT-PCR was used to measure the mRNA expression of IL-1β and TNF-α in the hippocampi of all groups; western blot was used to measure the protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, p38, and MKP-1 in the hippocampi of all groups at weeks 1, 2, 3, and 4 after modeling. Results. At weeks 1, 2, 3, and 4 after modeling, there were no significant differences between the control group and the DOPS group in the mRNA and protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, p38, and MKP-1 (all P>0.05); the mRNA and protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, and p38 were significantly increased, while the phosphorylation of MKP-1 was decreased in the model group compared with the control group. The mRNA and protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, and p38 were significantly decreased, while the phosphorylation of MKP-1 was increased in the DOPS intervention group compared with the model group. Conclusion. DOPS can reduce PTZ-induced brain inflammation and seizures of epileptic rats by inhibiting IL-1β, TNF-α, and MAPK signal pathways.http://dx.doi.org/10.1155/2019/9058161 |
| spellingShingle | Lin Zhang Hua Peng Jin Xu Yixin Xu You Yin Bin He Jianhua Zhuang Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats International Journal of Polymer Science |
| title | Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats |
| title_full | Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats |
| title_fullStr | Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats |
| title_full_unstemmed | Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats |
| title_short | Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats |
| title_sort | effects of dendrobium officinale polysaccharides on brain inflammation of epileptic rats |
| url | http://dx.doi.org/10.1155/2019/9058161 |
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