Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring
This study investigated whether a high Met diet influences biochemical parameters, MMPs activities in plasma, and biochemical and histological remodeling in aorta, in both pregnant female rabbits and their offspring. Four female rabbit groups are constituted (each n=8), nonpregnant control (NPC), pr...
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| Format: | Article |
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Wiley
2017-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2017/2785142 |
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| author | Khira Othmani Mecif Souhila Aouichat Bouguerra Yasmina Benazzoug |
| author_facet | Khira Othmani Mecif Souhila Aouichat Bouguerra Yasmina Benazzoug |
| author_sort | Khira Othmani Mecif |
| collection | DOAJ |
| description | This study investigated whether a high Met diet influences biochemical parameters, MMPs activities in plasma, and biochemical and histological remodeling in aorta, in both pregnant female rabbits and their offspring. Four female rabbit groups are constituted (each n=8), nonpregnant control (NPC), pregnant control (PC) that received normal commercial chow, nonpregnant Met (NPMet), and pregnant Met (PMet) that received the same diet supplemented with 0,35% L-methionine (w/w) for 3 months (500 mg/d). All pregnant females realize 3 successive pregnancies. Plasma results showed that Met excess increased Hcy, raised CRP in NPMet and decreased it in PMet, enhanced significantly proMMP-2 and proMMP-9 activities in NPMet, and reduced them in PMet. Aorta showed a rise in collagen level, essentially in PMet, a reduction of elastin content in both PMet and NPMet, and a significant decrease in lipid content in PMet, with histological changes that are more pronounced in NPMet than PMet. Met excess enhanced proMMP-9 activities in NPMet while it decreased them in PMet. PMet newborn presented increase in uremia and CRP and significant rise of active MMP-2 and MMP-9 forms. In aorta, media and adventitia thickness increased, total lipids content decreased, proMMP-9 activity decreased, and proMMP-2 activity increased. |
| format | Article |
| id | doaj-art-6c8c388f5fa845478dff375cbc938c40 |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-6c8c388f5fa845478dff375cbc938c402025-02-03T01:21:24ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322017-01-01201710.1155/2017/27851422785142Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their OffspringKhira Othmani Mecif0Souhila Aouichat Bouguerra1Yasmina Benazzoug2Laboratory of Cellular and Molecular Biology, Biochemistry of Extracellular Matrix Remodeling, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, El Alia, 16011 Algiers, AlgeriaLaboratory of Physiology of Organisms, Team of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, EL Alia, 16011 Algiers, AlgeriaLaboratory of Cellular and Molecular Biology, Biochemistry of Extracellular Matrix Remodeling, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, El Alia, 16011 Algiers, AlgeriaThis study investigated whether a high Met diet influences biochemical parameters, MMPs activities in plasma, and biochemical and histological remodeling in aorta, in both pregnant female rabbits and their offspring. Four female rabbit groups are constituted (each n=8), nonpregnant control (NPC), pregnant control (PC) that received normal commercial chow, nonpregnant Met (NPMet), and pregnant Met (PMet) that received the same diet supplemented with 0,35% L-methionine (w/w) for 3 months (500 mg/d). All pregnant females realize 3 successive pregnancies. Plasma results showed that Met excess increased Hcy, raised CRP in NPMet and decreased it in PMet, enhanced significantly proMMP-2 and proMMP-9 activities in NPMet, and reduced them in PMet. Aorta showed a rise in collagen level, essentially in PMet, a reduction of elastin content in both PMet and NPMet, and a significant decrease in lipid content in PMet, with histological changes that are more pronounced in NPMet than PMet. Met excess enhanced proMMP-9 activities in NPMet while it decreased them in PMet. PMet newborn presented increase in uremia and CRP and significant rise of active MMP-2 and MMP-9 forms. In aorta, media and adventitia thickness increased, total lipids content decreased, proMMP-9 activity decreased, and proMMP-2 activity increased.http://dx.doi.org/10.1155/2017/2785142 |
| spellingShingle | Khira Othmani Mecif Souhila Aouichat Bouguerra Yasmina Benazzoug Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring Journal of Nutrition and Metabolism |
| title | Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring |
| title_full | Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring |
| title_fullStr | Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring |
| title_full_unstemmed | Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring |
| title_short | Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring |
| title_sort | plasma and aorta biochemistry and mmps activities in female rabbit fed methionine enriched diet and their offspring |
| url | http://dx.doi.org/10.1155/2017/2785142 |
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