CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration
Neutrophils, known as an important part of the immune system, are the most abundant leukocyte population in peripheral blood, but excessive recruitment will lead to tissue/organ injury. RNA sequencing showed that ionizing radiation significantly increased the expression of characteristic genes of ne...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/7966089 |
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| _version_ | 1832558390282289152 |
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| author | Jing Hu Qian Ji Fei Chen Xiaoqin Gong Chuansheng Chen Kaijun Zhang Ye Hua Jinlei Wang Rongkun Li Xu Wang Chunhua Dai Ye Tian |
| author_facet | Jing Hu Qian Ji Fei Chen Xiaoqin Gong Chuansheng Chen Kaijun Zhang Ye Hua Jinlei Wang Rongkun Li Xu Wang Chunhua Dai Ye Tian |
| author_sort | Jing Hu |
| collection | DOAJ |
| description | Neutrophils, known as an important part of the immune system, are the most abundant leukocyte population in peripheral blood, but excessive recruitment will lead to tissue/organ injury. RNA sequencing showed that ionizing radiation significantly increased the expression of characteristic genes of neutrophils in intestinal tissues compared with liver and lung tissues. By clearing neutrophils with an anti-Ly6G antibody, we found that neutrophil infiltration is critical for irradiation-induced intestinal injury. CXCR2 is a G-protein-coupled receptor that mediates the migration of neutrophils by combining with its ligands. Compared with observations in liver and lung tissues, we found that CXCR2 and its ligands, including CXCL1, CXCL2, CXCL3, and CXCL5, were all significantly upregulated in irradiated intestinal tissues. Further studies showed that SB225002, an inhibitor of CXCR2, could effectively inhibit the chemotaxis of neutrophils and tissue damage mediated by the CXCL-CXCR2 signalling pathway. |
| format | Article |
| id | doaj-art-6c8af92bcea84e61866ba01b17f478ab |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6c8af92bcea84e61866ba01b17f478ab2025-02-03T01:32:27ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/7966089CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil InfiltrationJing Hu0Qian Ji1Fei Chen2Xiaoqin Gong3Chuansheng Chen4Kaijun Zhang5Ye Hua6Jinlei Wang7Rongkun Li8Xu Wang9Chunhua Dai10Ye Tian11Department of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyNeutrophils, known as an important part of the immune system, are the most abundant leukocyte population in peripheral blood, but excessive recruitment will lead to tissue/organ injury. RNA sequencing showed that ionizing radiation significantly increased the expression of characteristic genes of neutrophils in intestinal tissues compared with liver and lung tissues. By clearing neutrophils with an anti-Ly6G antibody, we found that neutrophil infiltration is critical for irradiation-induced intestinal injury. CXCR2 is a G-protein-coupled receptor that mediates the migration of neutrophils by combining with its ligands. Compared with observations in liver and lung tissues, we found that CXCR2 and its ligands, including CXCL1, CXCL2, CXCL3, and CXCL5, were all significantly upregulated in irradiated intestinal tissues. Further studies showed that SB225002, an inhibitor of CXCR2, could effectively inhibit the chemotaxis of neutrophils and tissue damage mediated by the CXCL-CXCR2 signalling pathway.http://dx.doi.org/10.1155/2022/7966089 |
| spellingShingle | Jing Hu Qian Ji Fei Chen Xiaoqin Gong Chuansheng Chen Kaijun Zhang Ye Hua Jinlei Wang Rongkun Li Xu Wang Chunhua Dai Ye Tian CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration Journal of Immunology Research |
| title | CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration |
| title_full | CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration |
| title_fullStr | CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration |
| title_full_unstemmed | CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration |
| title_short | CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration |
| title_sort | cxcr2 is essential for radiation induced intestinal injury by initiating neutrophil infiltration |
| url | http://dx.doi.org/10.1155/2022/7966089 |
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