Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques
Stroke induces a robust inflammatory response. However, it still lacks a systematic view of the various immune cell types due to the limited numbers of fluorophore used in the traditional FACS technique. In our current study, we utilized the novel technique mass cytometry (CyTOF) to analyze multiple...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/9132410 |
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| author | Yaning Li Yan Wang Yang Yao Brian B. Griffiths Liangshu Feng Tao Tao Feng Wang Baohui Xu Creed M. Stary Heng Zhao |
| author_facet | Yaning Li Yan Wang Yang Yao Brian B. Griffiths Liangshu Feng Tao Tao Feng Wang Baohui Xu Creed M. Stary Heng Zhao |
| author_sort | Yaning Li |
| collection | DOAJ |
| description | Stroke induces a robust inflammatory response. However, it still lacks a systematic view of the various immune cell types due to the limited numbers of fluorophore used in the traditional FACS technique. In our current study, we utilized the novel technique mass cytometry (CyTOF) to analyze multiple immune cell types. We detected these immune cells from the ischemic brain, peripheral blood, spleen, and bone marrow at different time courses after stroke. Our data showed (1) dynamic changes in the immune cell numbers in the ischemic brain and peripheral organs. (2) The expression levels of cell surface markers indicate the inflammation response status after stroke. Interestingly, CD62L, a key adhesion molecule, regulates the migration of leukocytes from blood vessels into secondary lymphoid tissues and peripheral tissues. (3) A strong leukocyte network across the brain and peripheral immune organs was identified using the R program at day 1 after ischemia, suggesting that the peripheral immune cells dramatically migrated into the ischemic areas after stroke. This study provides a systematic, wide view of the immune components in the brain and peripheral organs for a deep understanding of the immune response after ischemic stroke. |
| format | Article |
| id | doaj-art-6c494e0b2a7245faa53f4d01f8895b95 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6c494e0b2a7245faa53f4d01f8895b952025-02-03T05:49:53ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/91324109132410Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF TechniquesYaning Li0Yan Wang1Yang Yao2Brian B. Griffiths3Liangshu Feng4Tao Tao5Feng Wang6Baohui Xu7Creed M. Stary8Heng Zhao9Department of Neurosurgery, School of Medicine, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, School of Medicine, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, School of Medicine, Stanford University, Stanford, CA 94305, USADepartment of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, CA 94305, USADepartment of Neurosurgery, School of Medicine, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, School of Medicine, Stanford University, Stanford, CA 94305, USACenter for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDivision of Vascular Surgery, School of Medicine, Stanford University, Stanford, CA 94305, USADepartment of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, CA 94305, USADepartment of Neurosurgery, School of Medicine, Stanford University, Stanford, CA 94305, USAStroke induces a robust inflammatory response. However, it still lacks a systematic view of the various immune cell types due to the limited numbers of fluorophore used in the traditional FACS technique. In our current study, we utilized the novel technique mass cytometry (CyTOF) to analyze multiple immune cell types. We detected these immune cells from the ischemic brain, peripheral blood, spleen, and bone marrow at different time courses after stroke. Our data showed (1) dynamic changes in the immune cell numbers in the ischemic brain and peripheral organs. (2) The expression levels of cell surface markers indicate the inflammation response status after stroke. Interestingly, CD62L, a key adhesion molecule, regulates the migration of leukocytes from blood vessels into secondary lymphoid tissues and peripheral tissues. (3) A strong leukocyte network across the brain and peripheral immune organs was identified using the R program at day 1 after ischemia, suggesting that the peripheral immune cells dramatically migrated into the ischemic areas after stroke. This study provides a systematic, wide view of the immune components in the brain and peripheral organs for a deep understanding of the immune response after ischemic stroke.http://dx.doi.org/10.1155/2020/9132410 |
| spellingShingle | Yaning Li Yan Wang Yang Yao Brian B. Griffiths Liangshu Feng Tao Tao Feng Wang Baohui Xu Creed M. Stary Heng Zhao Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques Journal of Immunology Research |
| title | Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques |
| title_full | Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques |
| title_fullStr | Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques |
| title_full_unstemmed | Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques |
| title_short | Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques |
| title_sort | systematic study of the immune components after ischemic stroke using cytof techniques |
| url | http://dx.doi.org/10.1155/2020/9132410 |
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