Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method
Tiliroside exhibits a wide spectrum of effects on the human body; considering expensive synthesis of tiliroside, linden trees seem to be a good source of this compound. For the first time, 46 various extraction methods were developed to receive tiliroside from Tilia L., including ultrasound-assisted...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/9052425 |
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| author | Aleksandra Pieczykolan Wioleta Pietrzak Renata Nowak Józefina Pielczyk Karolina Łamacz |
| author_facet | Aleksandra Pieczykolan Wioleta Pietrzak Renata Nowak Józefina Pielczyk Karolina Łamacz |
| author_sort | Aleksandra Pieczykolan |
| collection | DOAJ |
| description | Tiliroside exhibits a wide spectrum of effects on the human body; considering expensive synthesis of tiliroside, linden trees seem to be a good source of this compound. For the first time, 46 various extraction methods were developed to receive tiliroside from Tilia L., including ultrasound-assisted extraction, maceration, maceration with stirring, accelerated solvent extraction, and extraction under reflux. The effects of extraction techniques, solvents, additives, and temperature on the content of tiliroside were studied using analytical and statistical methods. A new, rapid, simple, sensitive, and selective liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to determine the content of tiliroside in Tilia L. flowers. The LC-ESI-MS/MS analysis demonstrated the highest content of tiliroside in Tilia L. flowers obtained using accelerated solvent extraction (ASE) where 70% ethanol with addition of 1% acetic acid was used as a solvent (7.400 ± 0.019 mg of tiliroside per g dry extract).The results showed that the extracts of Tiliae inflorescentia contained large amounts of tiliroside; therefore, they are good sources of this compound. Moreover, ASE was found to be superior to other extraction techniques due to its high efficiency as well as considerable saving of time and solvent. |
| format | Article |
| id | doaj-art-6beeb8662bbd4754b7dd65c264e16fca |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-6beeb8662bbd4754b7dd65c264e16fca2025-02-03T01:10:22ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/90524259052425Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS MethodAleksandra Pieczykolan0Wioleta Pietrzak1Renata Nowak2Józefina Pielczyk3Karolina Łamacz4Department of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1 Str., 20-093 Lublin, PolandDepartment of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1 Str., 20-093 Lublin, PolandDepartment of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1 Str., 20-093 Lublin, PolandDepartment of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1 Str., 20-093 Lublin, PolandDepartment of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1 Str., 20-093 Lublin, PolandTiliroside exhibits a wide spectrum of effects on the human body; considering expensive synthesis of tiliroside, linden trees seem to be a good source of this compound. For the first time, 46 various extraction methods were developed to receive tiliroside from Tilia L., including ultrasound-assisted extraction, maceration, maceration with stirring, accelerated solvent extraction, and extraction under reflux. The effects of extraction techniques, solvents, additives, and temperature on the content of tiliroside were studied using analytical and statistical methods. A new, rapid, simple, sensitive, and selective liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to determine the content of tiliroside in Tilia L. flowers. The LC-ESI-MS/MS analysis demonstrated the highest content of tiliroside in Tilia L. flowers obtained using accelerated solvent extraction (ASE) where 70% ethanol with addition of 1% acetic acid was used as a solvent (7.400 ± 0.019 mg of tiliroside per g dry extract).The results showed that the extracts of Tiliae inflorescentia contained large amounts of tiliroside; therefore, they are good sources of this compound. Moreover, ASE was found to be superior to other extraction techniques due to its high efficiency as well as considerable saving of time and solvent.http://dx.doi.org/10.1155/2019/9052425 |
| spellingShingle | Aleksandra Pieczykolan Wioleta Pietrzak Renata Nowak Józefina Pielczyk Karolina Łamacz Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method Journal of Analytical Methods in Chemistry |
| title | Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method |
| title_full | Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method |
| title_fullStr | Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method |
| title_full_unstemmed | Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method |
| title_short | Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method |
| title_sort | optimization of extraction conditions for determination of tiliroside in tilia l flowers using an lc esi ms ms method |
| url | http://dx.doi.org/10.1155/2019/9052425 |
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