Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma
Abstract Objective This study aimed to investigate the role of the autophagy-related long noncoding RNA (lncRNA) MIR210HG in hepatocellular carcinoma and its potential as a therapeutic target. Methods LncRNA MIR210HG expression and its correlation with survival outcomes in hepatocellular carcinoma p...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-01765-3 |
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| _version_ | 1832585642124509184 |
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| author | Chaoqun Zhang Dianxing Sun Huifang Zhou Chao Liu Jie Ruan Jiwen Kang Ying Xie |
| author_facet | Chaoqun Zhang Dianxing Sun Huifang Zhou Chao Liu Jie Ruan Jiwen Kang Ying Xie |
| author_sort | Chaoqun Zhang |
| collection | DOAJ |
| description | Abstract Objective This study aimed to investigate the role of the autophagy-related long noncoding RNA (lncRNA) MIR210HG in hepatocellular carcinoma and its potential as a therapeutic target. Methods LncRNA MIR210HG expression and its correlation with survival outcomes in hepatocellular carcinoma patients were analyzed using data from The Cancer Genome Atlas (TCGA). Kaplan–Meier and Cox regression analyses were conducted to assess survival correlations. Quantitative reverse transcription PCR was used to measure lncRNA MIR210HG expression in liver cancer cells and normal liver cells. Functional assays, including CCK-8, Transwell, flow cytometry, and western blot, were performed to evaluate the effects of lncRNA MIR210HG on cell proliferation, invasion, apoptosis, and autophagy in hepatocellular carcinoma. Results Elevated lncRNA MIR210HG expression correlated with poor overall survival in hepatocellular carcinoma patients. LncRNA MIR210HG expression was significantly up-regulated in hepatocellular carcinoma cells compared to normal liver cells. Knockdown of lncRNA MIR210HG inhibited cell proliferation and autophagy, while promoting apoptosis in hepatocellular carcinoma cells, findings that were confirmed through both in vitro and in vivo studies. Conclusion The findings suggest that lncRNA MIR210HG contributes to hepatocellular carcinoma progression by regulating autophagy and could serve as a promising therapeutic target in hepatocellular carcinoma treatment strategies. |
| format | Article |
| id | doaj-art-6bd7804094094da1a3f19be9ca6d34ac |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-6bd7804094094da1a3f19be9ca6d34ac2025-01-26T12:39:48ZengSpringerDiscover Oncology2730-60112025-01-0116111410.1007/s12672-025-01765-3Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinomaChaoqun Zhang0Dianxing Sun1Huifang Zhou2Chao Liu3Jie Ruan4Jiwen Kang5Ying Xie6Hebei Key Laboratory of Laboratory Animal Science, Hebei Medical UniversityHebei Key Laboratory of Laboratory Animal Science, Hebei Medical UniversityDepartment of Infection, The 980, Hospital of PLA Joint Logistics Support ForceHebei Key Laboratory of Laboratory Animal Science, Hebei Medical UniversityHebei Key Laboratory of Laboratory Animal Science, Hebei Medical UniversityDepartment of Infection, The 980, Hospital of PLA Joint Logistics Support ForceHebei Key Laboratory of Laboratory Animal Science, Hebei Medical UniversityAbstract Objective This study aimed to investigate the role of the autophagy-related long noncoding RNA (lncRNA) MIR210HG in hepatocellular carcinoma and its potential as a therapeutic target. Methods LncRNA MIR210HG expression and its correlation with survival outcomes in hepatocellular carcinoma patients were analyzed using data from The Cancer Genome Atlas (TCGA). Kaplan–Meier and Cox regression analyses were conducted to assess survival correlations. Quantitative reverse transcription PCR was used to measure lncRNA MIR210HG expression in liver cancer cells and normal liver cells. Functional assays, including CCK-8, Transwell, flow cytometry, and western blot, were performed to evaluate the effects of lncRNA MIR210HG on cell proliferation, invasion, apoptosis, and autophagy in hepatocellular carcinoma. Results Elevated lncRNA MIR210HG expression correlated with poor overall survival in hepatocellular carcinoma patients. LncRNA MIR210HG expression was significantly up-regulated in hepatocellular carcinoma cells compared to normal liver cells. Knockdown of lncRNA MIR210HG inhibited cell proliferation and autophagy, while promoting apoptosis in hepatocellular carcinoma cells, findings that were confirmed through both in vitro and in vivo studies. Conclusion The findings suggest that lncRNA MIR210HG contributes to hepatocellular carcinoma progression by regulating autophagy and could serve as a promising therapeutic target in hepatocellular carcinoma treatment strategies.https://doi.org/10.1007/s12672-025-01765-3AutophagyHepatocellular carcinomaLiver cancerLong non-coding RNALncRNA MIR210HGPrognostic model; survival |
| spellingShingle | Chaoqun Zhang Dianxing Sun Huifang Zhou Chao Liu Jie Ruan Jiwen Kang Ying Xie Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma Discover Oncology Autophagy Hepatocellular carcinoma Liver cancer Long non-coding RNA LncRNA MIR210HG Prognostic model; survival |
| title | Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma |
| title_full | Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma |
| title_fullStr | Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma |
| title_full_unstemmed | Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma |
| title_short | Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma |
| title_sort | autophagy related long non coding rna mir210hg plays a therapeutic role in hepatocellular carcinoma |
| topic | Autophagy Hepatocellular carcinoma Liver cancer Long non-coding RNA LncRNA MIR210HG Prognostic model; survival |
| url | https://doi.org/10.1007/s12672-025-01765-3 |
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