Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Conditions
The high pathogenic activity of <i>Bacillus thuringiensis</i> (<i>Bt</i>) strains against various insect orders has positioned it as the most effective, environmentally safe, and sustainable approach to integrated insect management. We aimed to identify <i>Bt</i>...
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2024-12-01
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| author | Tatiane Aparecida Nascimento Maria José Paes Fernando Hercos Valicente Margareth Maria de Carvalho Queiroz |
| author_facet | Tatiane Aparecida Nascimento Maria José Paes Fernando Hercos Valicente Margareth Maria de Carvalho Queiroz |
| author_sort | Tatiane Aparecida Nascimento |
| collection | DOAJ |
| description | The high pathogenic activity of <i>Bacillus thuringiensis</i> (<i>Bt</i>) strains against various insect orders has positioned it as the most effective, environmentally safe, and sustainable approach to integrated insect management. We aimed to identify <i>Bt</i> strains capable of effectively controlling the housefly <i>Musca domestica</i>, a species of significant medical, veterinary, and public health concern. Twelve <i>Bt</i> strains from Macapá, Brazil, were tested against housefly larvae. PCR was employed to detect genes encoding Cry and Cyt proteins and Vips. Six strains exhibited 70 to 100% larval mortality, with five containing <i>cry</i> genes, none harboring <i>cyt</i> genes, and four carrying <i>vip</i> genes. Scanning electron microscopy revealed the production of crystal inclusions with distinct morphologies: spherical (TR4J, SOL5DM, SOL6RN), cuboidal and bipyramidal (TRO1TN and TRO2MQ), and spherical and bipyramidal (UNI2MA). The potential presence of genes from the <i>cry1</i>, <i>cry2</i>, <i>vip1</i>, and <i>vip3</i> families suggests that these strains also exhibit bioinsecticidal activity against other muscoid flies and insect pests across various orders. This study underscores the bioactive potential of these Brazilian <i>Bt</i> strains for developing new bioinsecticides. |
| format | Article |
| id | doaj-art-6bbe6dcf84284257bae74c59bae97953 |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-6bbe6dcf84284257bae74c59bae979532025-01-24T13:35:38ZengMDPI AGInsects2075-44502024-12-011612710.3390/insects16010027Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory ConditionsTatiane Aparecida Nascimento0Maria José Paes1Fernando Hercos Valicente2Margareth Maria de Carvalho Queiroz3Programa de Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz–Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro 21040-900, RJ, BrazilLaboratório Integrado: Simulídeos e Oncocercose & Entomologia Médica e Forense—LSOEMF, Instituto Oswaldo Cruz–Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro 21040-900, RJ, BrazilEmbrapa Milho e Sorgo, Sete Lagoas 35701-970, MG, BrazilPrograma de Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz–Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro 21040-900, RJ, BrazilThe high pathogenic activity of <i>Bacillus thuringiensis</i> (<i>Bt</i>) strains against various insect orders has positioned it as the most effective, environmentally safe, and sustainable approach to integrated insect management. We aimed to identify <i>Bt</i> strains capable of effectively controlling the housefly <i>Musca domestica</i>, a species of significant medical, veterinary, and public health concern. Twelve <i>Bt</i> strains from Macapá, Brazil, were tested against housefly larvae. PCR was employed to detect genes encoding Cry and Cyt proteins and Vips. Six strains exhibited 70 to 100% larval mortality, with five containing <i>cry</i> genes, none harboring <i>cyt</i> genes, and four carrying <i>vip</i> genes. Scanning electron microscopy revealed the production of crystal inclusions with distinct morphologies: spherical (TR4J, SOL5DM, SOL6RN), cuboidal and bipyramidal (TRO1TN and TRO2MQ), and spherical and bipyramidal (UNI2MA). The potential presence of genes from the <i>cry1</i>, <i>cry2</i>, <i>vip1</i>, and <i>vip3</i> families suggests that these strains also exhibit bioinsecticidal activity against other muscoid flies and insect pests across various orders. This study underscores the bioactive potential of these Brazilian <i>Bt</i> strains for developing new bioinsecticides.https://www.mdpi.com/2075-4450/16/1/27biological controlbacteriamuscoid fliespest insectsvectorultrastructure |
| spellingShingle | Tatiane Aparecida Nascimento Maria José Paes Fernando Hercos Valicente Margareth Maria de Carvalho Queiroz Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Conditions Insects biological control bacteria muscoid flies pest insects vector ultrastructure |
| title | Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Conditions |
| title_full | Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Conditions |
| title_fullStr | Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Conditions |
| title_full_unstemmed | Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Conditions |
| title_short | Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Conditions |
| title_sort | bioactive potential of some i bacillus thuringiensis i strains from macapa amazon brazil against the housefly i musca domestica i diptera muscidae under laboratory conditions |
| topic | biological control bacteria muscoid flies pest insects vector ultrastructure |
| url | https://www.mdpi.com/2075-4450/16/1/27 |
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