Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats
Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administratio...
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Wiley
2016-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2016/3126280 |
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| author | Artur Dembiński Zygmunt Warzecha Piotr Ceranowicz Marcin Dembiński Jakub Cieszkowski Tomasz Gosiewski Małgorzata Bulanda Beata Kuśnierz-Cabala Krystyna Gałązka Peter Christopher Konturek |
| author_facet | Artur Dembiński Zygmunt Warzecha Piotr Ceranowicz Marcin Dembiński Jakub Cieszkowski Tomasz Gosiewski Małgorzata Bulanda Beata Kuśnierz-Cabala Krystyna Gałązka Peter Christopher Konturek |
| author_sort | Artur Dembiński |
| collection | DOAJ |
| description | Background. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (109 CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats. |
| format | Article |
| id | doaj-art-6bb14e9eeb6a49058ea8e688c3f06991 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-6bb14e9eeb6a49058ea8e688c3f069912025-02-03T05:53:59ZengWileyGastroenterology Research and Practice1687-61211687-630X2016-01-01201610.1155/2016/31262803126280Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in RatsArtur Dembiński0Zygmunt Warzecha1Piotr Ceranowicz2Marcin Dembiński3Jakub Cieszkowski4Tomasz Gosiewski5Małgorzata Bulanda6Beata Kuśnierz-Cabala7Krystyna Gałązka8Peter Christopher Konturek9Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, PolandDepartment of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, PolandDepartment of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, PolandThe Second Department of General Surgery, Faculty of Medicine, Jagiellonian University Medical College, 21 Kopernika Street, 31-501 Cracow, PolandDepartment of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, PolandDepartment of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Cracow, PolandDepartment of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Cracow, PolandDepartment of Diagnostics, Chair of Clinical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, 15a Kopernika Street, 31-501 Cracow, PolandDepartment of Pathomorphology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531 Cracow, PolandDepartment of Internal Medicine II, Thuringia Clinic, Teaching Hospital of the University of Jena, Rainweg 68, 07318 Saalfeld, GermanyBackground. Inflammatory bowel disease results from the dysregulation of immune response to environmental and microbial agents in genetically susceptible individuals. The aim of the present study was to examine the effect of rifaximin and/or Mutaflor (Escherichia coli Nissle 1917, EcN) administration on the healing of acetic acid-induced colitis. Methods. Colitis was induced in male Wistar rats by rectal enema with 3.5% acetic acid solution. Rifaximin (50 mg/kg/dose) and/or Mutaflor (109 CFU/dose) were given intragastrically once a day. The severity of colitis was assessed at the 8th day after induction of inflammation. Results. Treatment with rifaximin significantly accelerated the healing of colonic damage. This effect was associated with significant reversion of the acetic acid-evoked decrease in mucosal blood flow and DNA synthesis. Moreover, administration of rifaximin significantly reduced concentration of proinflammatory TNF-α and activity of myeloperoxidase in colonic mucosa. Mutaflor given alone was without significant effect on activity of colitis. In contrast, Mutaflor given in combination with rifaximin significantly enhanced therapeutic effect of rifaximin. Moreover, Mutaflor led to settle of the colon by EcN and this effect was augmented by pretreatment with rifaximin. Conclusion. Rifaximin and Mutaflor exhibit synergic anti-inflammatory and therapeutic effect in acetic acid-induced colitis in rats.http://dx.doi.org/10.1155/2016/3126280 |
| spellingShingle | Artur Dembiński Zygmunt Warzecha Piotr Ceranowicz Marcin Dembiński Jakub Cieszkowski Tomasz Gosiewski Małgorzata Bulanda Beata Kuśnierz-Cabala Krystyna Gałązka Peter Christopher Konturek Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats Gastroenterology Research and Practice |
| title | Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats |
| title_full | Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats |
| title_fullStr | Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats |
| title_full_unstemmed | Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats |
| title_short | Synergic Interaction of Rifaximin and Mutaflor (Escherichia coli Nissle 1917) in the Treatment of Acetic Acid-Induced Colitis in Rats |
| title_sort | synergic interaction of rifaximin and mutaflor escherichia coli nissle 1917 in the treatment of acetic acid induced colitis in rats |
| url | http://dx.doi.org/10.1155/2016/3126280 |
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