Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29
Interleukin 10 (IL-10) is a synthetic inhibitor of human cytokines with immunomodulatory and anti-inflammatory effects. This study was designed to investigate the expression variation of IL-10 in the multiple sites including cortex, hippocampus, and lung tissues of neonatal hypoxic-ischemic (HI) rat...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2021/6622713 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832549799870595072 |
|---|---|
| author | Xue Bai Liu-Lin Xiong Chang-Le Fang Hao-Li Zhou Lu-Lu Xue Yue Hu Qing-Jie Xia Jia Liu Jun-Yan Zhang Ting-Hua Wang Si-Jin Yang |
| author_facet | Xue Bai Liu-Lin Xiong Chang-Le Fang Hao-Li Zhou Lu-Lu Xue Yue Hu Qing-Jie Xia Jia Liu Jun-Yan Zhang Ting-Hua Wang Si-Jin Yang |
| author_sort | Xue Bai |
| collection | DOAJ |
| description | Interleukin 10 (IL-10) is a synthetic inhibitor of human cytokines with immunomodulatory and anti-inflammatory effects. This study was designed to investigate the expression variation of IL-10 in the multiple sites including cortex, hippocampus, and lung tissues of neonatal hypoxic-ischemic (HI) rats and explore the crucial role of IL-10 in alleviating HI brain damage. In this study, neonatal Sprague-Dawley rats were subjected to the right common carotid artery ligation, followed by 2 h of hypoxia. The expression of IL-10 in the cortex, hippocampus, and lung tissues was measured with immunohistochemistry, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot (WB). Immunofluorescence double staining was performed to observe the localization of IL-10 in neurons and astrocytes. Moreover, not-targeting and targeting IL-10 siRNA lentivirus vectors were injected into the rats of the negative control (NC) and IL-10 group, respectively, and the mRNA levels of B-cell lymphoma 2 (Bcl-2) and endoplasmic reticulum protein 29 (ERp29) were detected by RT-qPCR following IL-10 silence. The results demonstrated that the IL-10 expression was markedly increased after HI and IL-10 were colocalized with neurons and astrocytes which were badly injured by HI insult. In addition, Bcl-2 and ERp29 were remarkably decreased following IL-10 mRNA interference compared with the NC group. Our findings revealed that IL-10 exerted its antiapoptotic and neuroprotective effects by regulating the expression of Bcl-2 and ERp29, indicating that IL-10 may be a promising molecule target for HIE treatment. |
| format | Article |
| id | doaj-art-6babac1c78304590ba12ea1fe1c902b5 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-6babac1c78304590ba12ea1fe1c902b52025-02-03T06:08:34ZengWileyAnalytical Cellular Pathology2210-71772210-71852021-01-01202110.1155/2021/66227136622713Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29Xue Bai0Liu-Lin Xiong1Chang-Le Fang2Hao-Li Zhou3Lu-Lu Xue4Yue Hu5Qing-Jie Xia6Jia Liu7Jun-Yan Zhang8Ting-Hua Wang9Si-Jin Yang10Department of Cardiac and Cerebral Diseases, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou 646000, ChinaSchool of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide 5000, AustraliaDepartment of Cardiac and Cerebral Diseases, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou 646000, ChinaInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, ChinaInstitute of Neuroscience, Animal Zoology Department, Kunming Medical University, Kunming 650031, ChinaInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, ChinaInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, ChinaInstitute of Neuroscience, Animal Zoology Department, Kunming Medical University, Kunming 650031, ChinaDepartment of Cardiac and Cerebral Diseases, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou 646000, ChinaInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Cardiac and Cerebral Diseases, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou 646000, ChinaInterleukin 10 (IL-10) is a synthetic inhibitor of human cytokines with immunomodulatory and anti-inflammatory effects. This study was designed to investigate the expression variation of IL-10 in the multiple sites including cortex, hippocampus, and lung tissues of neonatal hypoxic-ischemic (HI) rats and explore the crucial role of IL-10 in alleviating HI brain damage. In this study, neonatal Sprague-Dawley rats were subjected to the right common carotid artery ligation, followed by 2 h of hypoxia. The expression of IL-10 in the cortex, hippocampus, and lung tissues was measured with immunohistochemistry, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot (WB). Immunofluorescence double staining was performed to observe the localization of IL-10 in neurons and astrocytes. Moreover, not-targeting and targeting IL-10 siRNA lentivirus vectors were injected into the rats of the negative control (NC) and IL-10 group, respectively, and the mRNA levels of B-cell lymphoma 2 (Bcl-2) and endoplasmic reticulum protein 29 (ERp29) were detected by RT-qPCR following IL-10 silence. The results demonstrated that the IL-10 expression was markedly increased after HI and IL-10 were colocalized with neurons and astrocytes which were badly injured by HI insult. In addition, Bcl-2 and ERp29 were remarkably decreased following IL-10 mRNA interference compared with the NC group. Our findings revealed that IL-10 exerted its antiapoptotic and neuroprotective effects by regulating the expression of Bcl-2 and ERp29, indicating that IL-10 may be a promising molecule target for HIE treatment.http://dx.doi.org/10.1155/2021/6622713 |
| spellingShingle | Xue Bai Liu-Lin Xiong Chang-Le Fang Hao-Li Zhou Lu-Lu Xue Yue Hu Qing-Jie Xia Jia Liu Jun-Yan Zhang Ting-Hua Wang Si-Jin Yang Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 Analytical Cellular Pathology |
| title | Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 |
| title_full | Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 |
| title_fullStr | Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 |
| title_full_unstemmed | Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 |
| title_short | Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 |
| title_sort | interleukin 10 plays an important role in neonatal rats with hypoxic ischemia associated with b cell lymphoma 2 and endoplasmic reticulum protein 29 |
| url | http://dx.doi.org/10.1155/2021/6622713 |
| work_keys_str_mv | AT xuebai interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT liulinxiong interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT changlefang interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT haolizhou interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT luluxue interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT yuehu interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT qingjiexia interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT jialiu interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT junyanzhang interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT tinghuawang interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 AT sijinyang interleukin10playsanimportantroleinneonatalratswithhypoxicischemiaassociatedwithbcelllymphoma2andendoplasmicreticulumprotein29 |