Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells
This study examined the interaction of mouse bone marrow mesenchymal stem cells (MSC) with cardiac HL-1 cells during coculture by fluorescent dye labeling and then flow cytometry. MSC were layered onto confluent HL-1 cell cultures in a 1 : 4 ratio. MSC gained gap junction permeant calcein from HL-1...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/653734 |
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| _version_ | 1832567038115053568 |
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| author | Robert A. Boomsma David L. Geenen |
| author_facet | Robert A. Boomsma David L. Geenen |
| author_sort | Robert A. Boomsma |
| collection | DOAJ |
| description | This study examined the interaction of mouse bone marrow mesenchymal stem cells (MSC) with cardiac HL-1 cells during coculture by fluorescent dye labeling and then flow cytometry. MSC were layered onto confluent HL-1 cell cultures in a 1 : 4 ratio. MSC gained gap junction permeant calcein from HL-1 cells after 4 hours which was partially reduced by oleamide. After 20 hours, 99% MSC gained calcein, unaffected by oleamide. Double-labeling HL-1 cells with calcein and the membrane dye DiO resulted in transfer of both calcein and DiO to MSC. When HL-1 cells were labeled with calcein and MSC with DiO, MSC gained calcein while HL-1 cells gained DiO. Very little fusion was observed since more than 90% Sca-1 positive MSC gained DiO from HL-1 cells while less than 9% gained gap junction impermeant CMFDA after 20 hours with no Sca-1 transfer to HL-1 cells. Time dependent transfer of membrane DiD was observed from HL-1 cells to MSC (100%) and vice versa (50%) after 20 hours with more limited transfer of CMFDA. These results demonstrate that MSC and HL-1 cells exchange membrane components which may account for some of the beneficial effect of MSC in the heart after myocardial infarction. |
| format | Article |
| id | doaj-art-6ba8d21193ff44fd86680a07c1ae5dd3 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-6ba8d21193ff44fd86680a07c1ae5dd32025-02-03T01:02:27ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/653734653734Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac CellsRobert A. Boomsma0David L. Geenen1Department of Biology, Trinity Christian College, Palos Heights, IL 60463, USAPhysician Assistant Studies/Allied Health Sciences, College of Health Professions, Grand Valley State University, Grand Rapids, IL 49503, USAThis study examined the interaction of mouse bone marrow mesenchymal stem cells (MSC) with cardiac HL-1 cells during coculture by fluorescent dye labeling and then flow cytometry. MSC were layered onto confluent HL-1 cell cultures in a 1 : 4 ratio. MSC gained gap junction permeant calcein from HL-1 cells after 4 hours which was partially reduced by oleamide. After 20 hours, 99% MSC gained calcein, unaffected by oleamide. Double-labeling HL-1 cells with calcein and the membrane dye DiO resulted in transfer of both calcein and DiO to MSC. When HL-1 cells were labeled with calcein and MSC with DiO, MSC gained calcein while HL-1 cells gained DiO. Very little fusion was observed since more than 90% Sca-1 positive MSC gained DiO from HL-1 cells while less than 9% gained gap junction impermeant CMFDA after 20 hours with no Sca-1 transfer to HL-1 cells. Time dependent transfer of membrane DiD was observed from HL-1 cells to MSC (100%) and vice versa (50%) after 20 hours with more limited transfer of CMFDA. These results demonstrate that MSC and HL-1 cells exchange membrane components which may account for some of the beneficial effect of MSC in the heart after myocardial infarction.http://dx.doi.org/10.1155/2014/653734 |
| spellingShingle | Robert A. Boomsma David L. Geenen Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells Stem Cells International |
| title | Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells |
| title_full | Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells |
| title_fullStr | Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells |
| title_full_unstemmed | Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells |
| title_short | Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells |
| title_sort | evidence for transfer of membranes from mesenchymal stem cells to hl 1 cardiac cells |
| url | http://dx.doi.org/10.1155/2014/653734 |
| work_keys_str_mv | AT robertaboomsma evidencefortransferofmembranesfrommesenchymalstemcellstohl1cardiaccells AT davidlgeenen evidencefortransferofmembranesfrommesenchymalstemcellstohl1cardiaccells |