Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea
Calf diarrhea is a prevalent and significant health issue in dairy farming, severely impacting feed intake, weight gain, and survival rates in young calves. This study aimed to investigate the microbial composition and antibiotic resistance profiles of diarrheic calves to provide insights into the e...
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2024-12-01
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| author | Lu Zhang Jun Bai Qian Guo Long Li Yanqing Jia Xinxin Qiu Dong Zhou Zhencang Zhang Huafeng Niu |
| author_facet | Lu Zhang Jun Bai Qian Guo Long Li Yanqing Jia Xinxin Qiu Dong Zhou Zhencang Zhang Huafeng Niu |
| author_sort | Lu Zhang |
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| description | Calf diarrhea is a prevalent and significant health issue in dairy farming, severely impacting feed intake, weight gain, and survival rates in young calves. This study aimed to investigate the microbial composition and antibiotic resistance profiles of diarrheic calves to provide insights into the epidemiology and management of the condition. The prevalence of diarrhea in 1685 calves was analyzed. Rectal fecal samples were collected from five healthy and five diarrheic Holstein calves on a large dairy farm in Shaanxi Province, China. High-throughput 16S-rRNA sequencing and PCR were utilized for microbial and resistance gene analysis. In 2023, the overall diarrhea rate among 1685 calves was 9.08%, with a significantly higher diarrhea rate during the suckling period (8.13%) compared to the post-weaning period (0.95%) (<i>p</i> < 0.001). No differences in species diversity and richness were detected among the different groups. However, LEfSe analysis identified six genera (<i>Eubacterium, Eubacteriaceae, Prevotella, Comamonadaceae, Comamonas</i>, and <i>Firmicutes</i>) significantly enriched in diarrheic calves compared to healthy ones (LDA scores > 2, <i>p</i> < 0.05). Additionally, antibiotic resistance genes for quinolones, β-lactams, chloramphenicol, tetracyclines, and aminoglycosides were detected, with significantly higher prevalence in diarrheic calves. These findings demonstrate distinct microbial and antibiotic resistance profiles between healthy and diarrheic calves, emphasizing the importance of microbial management in controlling calf diarrhea. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj-art-6b9c97f5c0c54d12a5322e4ab46f5de42025-01-24T13:38:26ZengMDPI AGLife2075-17292024-12-011511010.3390/life15010010Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with DiarrheaLu Zhang0Jun Bai1Qian Guo2Long Li3Yanqing Jia4Xinxin Qiu5Dong Zhou6Zhencang Zhang7Huafeng Niu8Department of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, ChinaDepartment of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, ChinaShaanXi Province Management Station of Animal Health and Slaughter, Xi’an 710000, ChinaDepartment of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, ChinaDepartment of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, ChinaDepartment of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaDepartment of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, ChinaDepartment of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, ChinaCalf diarrhea is a prevalent and significant health issue in dairy farming, severely impacting feed intake, weight gain, and survival rates in young calves. This study aimed to investigate the microbial composition and antibiotic resistance profiles of diarrheic calves to provide insights into the epidemiology and management of the condition. The prevalence of diarrhea in 1685 calves was analyzed. Rectal fecal samples were collected from five healthy and five diarrheic Holstein calves on a large dairy farm in Shaanxi Province, China. High-throughput 16S-rRNA sequencing and PCR were utilized for microbial and resistance gene analysis. In 2023, the overall diarrhea rate among 1685 calves was 9.08%, with a significantly higher diarrhea rate during the suckling period (8.13%) compared to the post-weaning period (0.95%) (<i>p</i> < 0.001). No differences in species diversity and richness were detected among the different groups. However, LEfSe analysis identified six genera (<i>Eubacterium, Eubacteriaceae, Prevotella, Comamonadaceae, Comamonas</i>, and <i>Firmicutes</i>) significantly enriched in diarrheic calves compared to healthy ones (LDA scores > 2, <i>p</i> < 0.05). Additionally, antibiotic resistance genes for quinolones, β-lactams, chloramphenicol, tetracyclines, and aminoglycosides were detected, with significantly higher prevalence in diarrheic calves. These findings demonstrate distinct microbial and antibiotic resistance profiles between healthy and diarrheic calves, emphasizing the importance of microbial management in controlling calf diarrhea.https://www.mdpi.com/2075-1729/15/1/10calf diarrheamicrobiotahigh-throughput analysisresistant genes |
| spellingShingle | Lu Zhang Jun Bai Qian Guo Long Li Yanqing Jia Xinxin Qiu Dong Zhou Zhencang Zhang Huafeng Niu Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea Life calf diarrhea microbiota high-throughput analysis resistant genes |
| title | Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea |
| title_full | Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea |
| title_fullStr | Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea |
| title_full_unstemmed | Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea |
| title_short | Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea |
| title_sort | gut microbial composition and antibiotic resistance profiles in dairy calves with diarrhea |
| topic | calf diarrhea microbiota high-throughput analysis resistant genes |
| url | https://www.mdpi.com/2075-1729/15/1/10 |
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