Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans
Background. Dysregulated lipolysis has been implicated in mechanisms of cardiometabolic disease and inflammation in obesity. Purpose. We sought to examine the effect of bariatric weight loss on adipose tissue lipolytic gene expression and their relationship to systemic metabolic parameters in obese...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/106237 |
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| author | Shakun Karki Melissa G. Farb Samantha Myers Caroline Apovian Donald T. Hess Noyan Gokce |
| author_facet | Shakun Karki Melissa G. Farb Samantha Myers Caroline Apovian Donald T. Hess Noyan Gokce |
| author_sort | Shakun Karki |
| collection | DOAJ |
| description | Background. Dysregulated lipolysis has been implicated in mechanisms of cardiometabolic disease and inflammation in obesity. Purpose. We sought to examine the effect of bariatric weight loss on adipose tissue lipolytic gene expression and their relationship to systemic metabolic parameters in obese subjects. Methods/Results. We biopsied subcutaneous adipose tissue in 19 obese individuals (BMI 42 ± 5 kg/m2, 79% female) at baseline and after a mean period of 8 ± 5 months (range 3–15 months) following bariatric surgery. We performed adipose tissue mRNA expression of proteins involved in triglyceride hydrolysis and correlated their weight loss induced alterations with systemic parameters associated with cardiovascular disease risk. mRNA transcripts of adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and lipid droplet proteins comparative gene identification 58 (CGI-58) and perilipin increased significantly after weight loss (p<0.05 for all). ATGL expression correlated inversely with plasma triglyceride (TG), hemoglobin A1C (HbA1C), and glucose, and HSL expression correlated negatively with glucose, while CGI-58 was inversely associated with HbA1C. Conclusion. We observed increased expression of adipose tissue lipolytic genes following bariatric weight loss which correlated inversely with systemic markers of lipid and glucose metabolism. Functional alterations in lipolysis in human adipose tissue may play a role in shaping cardiometabolic phenotypes in human obesity. |
| format | Article |
| id | doaj-art-6b809f03332a418fa3df17d58842d557 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6b809f03332a418fa3df17d58842d5572025-02-03T01:27:45ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/106237106237Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese HumansShakun Karki0Melissa G. Farb1Samantha Myers2Caroline Apovian3Donald T. Hess4Noyan Gokce5Evans Department of Medicine and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USAEvans Department of Medicine and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USAEvans Department of Medicine and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USAEvans Department of Medicine and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USADepartment of General Surgery, Boston University School of Medicine, Boston, MA 02118, USAEvans Department of Medicine and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USABackground. Dysregulated lipolysis has been implicated in mechanisms of cardiometabolic disease and inflammation in obesity. Purpose. We sought to examine the effect of bariatric weight loss on adipose tissue lipolytic gene expression and their relationship to systemic metabolic parameters in obese subjects. Methods/Results. We biopsied subcutaneous adipose tissue in 19 obese individuals (BMI 42 ± 5 kg/m2, 79% female) at baseline and after a mean period of 8 ± 5 months (range 3–15 months) following bariatric surgery. We performed adipose tissue mRNA expression of proteins involved in triglyceride hydrolysis and correlated their weight loss induced alterations with systemic parameters associated with cardiovascular disease risk. mRNA transcripts of adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and lipid droplet proteins comparative gene identification 58 (CGI-58) and perilipin increased significantly after weight loss (p<0.05 for all). ATGL expression correlated inversely with plasma triglyceride (TG), hemoglobin A1C (HbA1C), and glucose, and HSL expression correlated negatively with glucose, while CGI-58 was inversely associated with HbA1C. Conclusion. We observed increased expression of adipose tissue lipolytic genes following bariatric weight loss which correlated inversely with systemic markers of lipid and glucose metabolism. Functional alterations in lipolysis in human adipose tissue may play a role in shaping cardiometabolic phenotypes in human obesity.http://dx.doi.org/10.1155/2015/106237 |
| spellingShingle | Shakun Karki Melissa G. Farb Samantha Myers Caroline Apovian Donald T. Hess Noyan Gokce Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans Mediators of Inflammation |
| title | Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans |
| title_full | Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans |
| title_fullStr | Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans |
| title_full_unstemmed | Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans |
| title_short | Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans |
| title_sort | effect of bariatric weight loss on the adipose lipolytic transcriptome in obese humans |
| url | http://dx.doi.org/10.1155/2015/106237 |
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