Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro
Human embryonic stem cells (hESCs) are being used extensively in array of studies to understand different mechanisms such as early human embryogenesis, drug toxicity testing, disease modeling, and cell replacement therapy. The protocols for the directed differentiation of hESCs towards specific cell...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/2451927 |
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| author | Divya S. Varghese Shama Parween Mustafa T. Ardah Bright Starling Emerald Suraiya A. Ansari |
| author_facet | Divya S. Varghese Shama Parween Mustafa T. Ardah Bright Starling Emerald Suraiya A. Ansari |
| author_sort | Divya S. Varghese |
| collection | DOAJ |
| description | Human embryonic stem cells (hESCs) are being used extensively in array of studies to understand different mechanisms such as early human embryogenesis, drug toxicity testing, disease modeling, and cell replacement therapy. The protocols for the directed differentiation of hESCs towards specific cell types often require long-term cell cultures. To avoid bacterial contamination, these protocols include addition of antibiotics such as pen-strep and gentamicin. Although aminoglycosides, streptomycin, and gentamicin have been shown to cause cytotoxicity in various animal models, the effect of these antibiotics on hESCs is not clear. In this study, we found that antibiotics, pen-strep, and gentamicin did not affect hESC cell viability or expression of pluripotency markers. However, during directed differentiation towards neural and hepatic fate, significant cell death was noted through the activation of caspase cascade. Also, the expression of neural progenitor markers Pax6, Emx2, Otx2, and Pou3f2 was significantly reduced suggesting that gentamicin may adversely affect early embryonic neurogenesis whereas no effect was seen on the expression of endoderm or hepatic markers during differentiation. Our results suggest that the use of antibiotics in cell culture media for the maintenance and differentiation of hESCs needs thorough investigation before use to avoid erroneous results. |
| format | Article |
| id | doaj-art-6b5dfb1d84e44c50b06b1564d5822078 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6b5dfb1d84e44c50b06b1564d58220782025-02-03T06:11:58ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/24519272451927Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In VitroDivya S. Varghese0Shama Parween1Mustafa T. Ardah2Bright Starling Emerald3Suraiya A. Ansari4Department of Biochemistry, College of Medicine and Health Sciences, UAE University, Al Ain, Abu Dhabi, UAEDepartment of Biochemistry, College of Medicine and Health Sciences, UAE University, Al Ain, Abu Dhabi, UAEDepartment of Biochemistry, College of Medicine and Health Sciences, UAE University, Al Ain, Abu Dhabi, UAEDepartment of Anatomy, College of Medicine and Health Sciences, UAE University, Al Ain, Abu Dhabi, UAEDepartment of Biochemistry, College of Medicine and Health Sciences, UAE University, Al Ain, Abu Dhabi, UAEHuman embryonic stem cells (hESCs) are being used extensively in array of studies to understand different mechanisms such as early human embryogenesis, drug toxicity testing, disease modeling, and cell replacement therapy. The protocols for the directed differentiation of hESCs towards specific cell types often require long-term cell cultures. To avoid bacterial contamination, these protocols include addition of antibiotics such as pen-strep and gentamicin. Although aminoglycosides, streptomycin, and gentamicin have been shown to cause cytotoxicity in various animal models, the effect of these antibiotics on hESCs is not clear. In this study, we found that antibiotics, pen-strep, and gentamicin did not affect hESC cell viability or expression of pluripotency markers. However, during directed differentiation towards neural and hepatic fate, significant cell death was noted through the activation of caspase cascade. Also, the expression of neural progenitor markers Pax6, Emx2, Otx2, and Pou3f2 was significantly reduced suggesting that gentamicin may adversely affect early embryonic neurogenesis whereas no effect was seen on the expression of endoderm or hepatic markers during differentiation. Our results suggest that the use of antibiotics in cell culture media for the maintenance and differentiation of hESCs needs thorough investigation before use to avoid erroneous results.http://dx.doi.org/10.1155/2017/2451927 |
| spellingShingle | Divya S. Varghese Shama Parween Mustafa T. Ardah Bright Starling Emerald Suraiya A. Ansari Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro Stem Cells International |
| title | Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro |
| title_full | Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro |
| title_fullStr | Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro |
| title_full_unstemmed | Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro |
| title_short | Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro |
| title_sort | effects of aminoglycoside antibiotics on human embryonic stem cell viability during differentiation in vitro |
| url | http://dx.doi.org/10.1155/2017/2451927 |
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