The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms
Legionella pneumophila is the pathogen responsible for severe pneumonia known as Legionnaires’ disease. Legionella can live under varied stress conditions, especially in cold environments, and is common in many artificial environments. In this study, the antimicrobial activity of biogenic silver nan...
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University of Tehran
2013-12-01
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| Series: | Journal of Sciences, Islamic Republic of Iran |
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| Online Access: | https://jsciences.ut.ac.ir/article_36449_7e67de4c7bdc4f9f173be3e29cb8c95e.pdf |
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| author | M.R. Pourmand K. Shahidi P. Nazari Seyed M. Moosavian N. Samadi Gh. Pourmand A. R. Shahverdi |
| author_facet | M.R. Pourmand K. Shahidi P. Nazari Seyed M. Moosavian N. Samadi Gh. Pourmand A. R. Shahverdi |
| author_sort | M.R. Pourmand |
| collection | DOAJ |
| description | Legionella pneumophila is the pathogen responsible for severe pneumonia known as Legionnaires’ disease. Legionella can live under varied stress conditions, especially in cold environments, and is common in many artificial environments. In this study, the antimicrobial activity of biogenic silver nanoparticles, prepared using the culture supernatant of Klebsiella pneumoniae, was evaluated against different important microorganisms, especially L. pneumophila. Compared with all bacterial test strains, L. pneumophila showed lower susceptibility against silver nanoparticles (MIC 30 µg/mL). The MICs of silver nanoparticles were 10 µg/ml for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, and Salmonella typhi. In addition, the MIC values of the silver nanoparticles for the fungal teststrain(Aspergillus niger and Candida albicans) were 20 and 30 µg/ml, respectively. The time-kill course antibacterial assay of silver nanoparticles at its MIC concentration (30 µg/mL) was performed against L. pneumophila at cold (4 °C) and ambient (25 °C) temperatures. The results showed that a higher mortality rate was observed at 4 °C. In contrast, at 25 °C,a considerable tolerance was observed for L. pneumophila against 30 µg/ml of silver nanoparticles during the 24-h exposure time. Reserpine as a natural efflux inhibitor (10 µg/mL) was used in this study to reverse the resistance of L. pneumophila against silver nanoparticles at 25 °C. Higher anti-legionella activity was detected in the presence of reserpine at the mentioned temperature that indicated that efflux pumps may have an important role in the resistance of L. pneumophila against silver nanoparticles at 25 °C. |
| format | Article |
| id | doaj-art-6b58bdcc714f451586ccf6b80a92cfde |
| institution | Kabale University |
| issn | 1016-1104 2345-6914 |
| language | English |
| publishDate | 2013-12-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Journal of Sciences, Islamic Republic of Iran |
| spelling | doaj-art-6b58bdcc714f451586ccf6b80a92cfde2025-08-20T03:53:51ZengUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-11042345-69142013-12-0124431331936449The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other MicroorganismsM.R. Pourmand0K. Shahidi1P. Nazari2Seyed M. Moosavian3N. Samadi4Gh. Pourmand5A. R. Shahverdi6Department of Pathobiology, School of Public Health, and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Microbiology, School of Medicine and Tropical Research Center, Ahwaz Jundishapour University of Medical Sciences, Ahwaz, Islamic Republic of IranDepartment of Drug and Food Control and Quality Assurance Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranUrology Research Center, Sina Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of IranLegionella pneumophila is the pathogen responsible for severe pneumonia known as Legionnaires’ disease. Legionella can live under varied stress conditions, especially in cold environments, and is common in many artificial environments. In this study, the antimicrobial activity of biogenic silver nanoparticles, prepared using the culture supernatant of Klebsiella pneumoniae, was evaluated against different important microorganisms, especially L. pneumophila. Compared with all bacterial test strains, L. pneumophila showed lower susceptibility against silver nanoparticles (MIC 30 µg/mL). The MICs of silver nanoparticles were 10 µg/ml for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, and Salmonella typhi. In addition, the MIC values of the silver nanoparticles for the fungal teststrain(Aspergillus niger and Candida albicans) were 20 and 30 µg/ml, respectively. The time-kill course antibacterial assay of silver nanoparticles at its MIC concentration (30 µg/mL) was performed against L. pneumophila at cold (4 °C) and ambient (25 °C) temperatures. The results showed that a higher mortality rate was observed at 4 °C. In contrast, at 25 °C,a considerable tolerance was observed for L. pneumophila against 30 µg/ml of silver nanoparticles during the 24-h exposure time. Reserpine as a natural efflux inhibitor (10 µg/mL) was used in this study to reverse the resistance of L. pneumophila against silver nanoparticles at 25 °C. Higher anti-legionella activity was detected in the presence of reserpine at the mentioned temperature that indicated that efflux pumps may have an important role in the resistance of L. pneumophila against silver nanoparticles at 25 °C.https://jsciences.ut.ac.ir/article_36449_7e67de4c7bdc4f9f173be3e29cb8c95e.pdfantimicrobial activityefflux pumphospital infectionlegionellasilver nanoparticles |
| spellingShingle | M.R. Pourmand K. Shahidi P. Nazari Seyed M. Moosavian N. Samadi Gh. Pourmand A. R. Shahverdi The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms Journal of Sciences, Islamic Republic of Iran antimicrobial activity efflux pump hospital infection legionella silver nanoparticles |
| title | The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms |
| title_full | The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms |
| title_fullStr | The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms |
| title_full_unstemmed | The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms |
| title_short | The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms |
| title_sort | different antibacterial impact of silver nanoparticles against legionella pneumophila compared to other microorganisms |
| topic | antimicrobial activity efflux pump hospital infection legionella silver nanoparticles |
| url | https://jsciences.ut.ac.ir/article_36449_7e67de4c7bdc4f9f173be3e29cb8c95e.pdf |
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