<i>Thermithiobacillus plumbiphilus</i> AAFK—Arsenic-Resistant Bacteria Isolated from Arsenopyrite Material
Autotrophic sulfur-oxidizing bacteria can play a key role in the metal bioleaching from low-grade sulfide-containing ores. The most commonly used bioleaching group is presented with acidophilic bacteria of the order <i>Acidithiobacillales</i>. We studied the diversity of bacteria in the...
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2025-01-01
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| author | Artem Shaikin Anton Zvonarev Mikhail Vainshtein Tatiana Abashina |
| author_facet | Artem Shaikin Anton Zvonarev Mikhail Vainshtein Tatiana Abashina |
| author_sort | Artem Shaikin |
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| description | Autotrophic sulfur-oxidizing bacteria can play a key role in the metal bioleaching from low-grade sulfide-containing ores. The most commonly used bioleaching group is presented with acidophilic bacteria of the order <i>Acidithiobacillales</i>. We studied the diversity of bacteria in the arsenopyrite gold-bearing ore and also discovered a wide distribution of neutrophilic non-thermophilic bacteria <i>Thermithiobacillus plumbiphilus</i> in this ore, as well as its drainage and flotation concentrate. For the first time, <i>T. plumbiphilus</i> was isolated from the natural arsenic-containing mineral material. The first description of complete genome for the species <i>T. plumbiphilus</i> was also carried out and discovered genes providing the As resistance. Culturing the isolated strain <i>T. plumbiphilus</i> AAFK confirmed the found bacterial resistance to arsenite and cocadylate during the effective thiosulfate oxidation. Experiments on the arsenopyrite bioleaching showed that <i>T. plumbiphilus</i> AAFK can be used as an auxiliary bacterial culture capable of oxidizing reduced / intermediate sulfur compounds. The genetic basis of the <i>T. plumbiphilus</i> AAFK resistance to the arsenic compounds is discussed; the mechanisms are similar with the ones known for acidophilic thiobacilli. The biofilm formation is shown for the first time for <i>T. plumbiphilus</i>; presumably, it could provide some protection and immobilization of the cells. Structures of the <i>T. plumbiphilus</i> AAFK cells and their production of outer membrane vesicles are described and discussed. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Microbiology Research |
| spelling | doaj-art-6b49fefdfa5f4be28bc2ac4c24e67a3a2025-01-24T13:41:44ZengMDPI AGMicrobiology Research2036-74812025-01-011611410.3390/microbiolres16010014<i>Thermithiobacillus plumbiphilus</i> AAFK—Arsenic-Resistant Bacteria Isolated from Arsenopyrite MaterialArtem Shaikin0Anton Zvonarev1Mikhail Vainshtein2Tatiana Abashina3G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Pushchino 142290, RussiaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Pushchino 142290, RussiaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Pushchino 142290, RussiaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Pushchino 142290, RussiaAutotrophic sulfur-oxidizing bacteria can play a key role in the metal bioleaching from low-grade sulfide-containing ores. The most commonly used bioleaching group is presented with acidophilic bacteria of the order <i>Acidithiobacillales</i>. We studied the diversity of bacteria in the arsenopyrite gold-bearing ore and also discovered a wide distribution of neutrophilic non-thermophilic bacteria <i>Thermithiobacillus plumbiphilus</i> in this ore, as well as its drainage and flotation concentrate. For the first time, <i>T. plumbiphilus</i> was isolated from the natural arsenic-containing mineral material. The first description of complete genome for the species <i>T. plumbiphilus</i> was also carried out and discovered genes providing the As resistance. Culturing the isolated strain <i>T. plumbiphilus</i> AAFK confirmed the found bacterial resistance to arsenite and cocadylate during the effective thiosulfate oxidation. Experiments on the arsenopyrite bioleaching showed that <i>T. plumbiphilus</i> AAFK can be used as an auxiliary bacterial culture capable of oxidizing reduced / intermediate sulfur compounds. The genetic basis of the <i>T. plumbiphilus</i> AAFK resistance to the arsenic compounds is discussed; the mechanisms are similar with the ones known for acidophilic thiobacilli. The biofilm formation is shown for the first time for <i>T. plumbiphilus</i>; presumably, it could provide some protection and immobilization of the cells. Structures of the <i>T. plumbiphilus</i> AAFK cells and their production of outer membrane vesicles are described and discussed.https://www.mdpi.com/2036-7481/16/1/14<i>Thermithiobacillus plumbiphilus</i>arsenopyritearsenic resistancesulfur compounds oxidation |
| spellingShingle | Artem Shaikin Anton Zvonarev Mikhail Vainshtein Tatiana Abashina <i>Thermithiobacillus plumbiphilus</i> AAFK—Arsenic-Resistant Bacteria Isolated from Arsenopyrite Material Microbiology Research <i>Thermithiobacillus plumbiphilus</i> arsenopyrite arsenic resistance sulfur compounds oxidation |
| title | <i>Thermithiobacillus plumbiphilus</i> AAFK—Arsenic-Resistant Bacteria Isolated from Arsenopyrite Material |
| title_full | <i>Thermithiobacillus plumbiphilus</i> AAFK—Arsenic-Resistant Bacteria Isolated from Arsenopyrite Material |
| title_fullStr | <i>Thermithiobacillus plumbiphilus</i> AAFK—Arsenic-Resistant Bacteria Isolated from Arsenopyrite Material |
| title_full_unstemmed | <i>Thermithiobacillus plumbiphilus</i> AAFK—Arsenic-Resistant Bacteria Isolated from Arsenopyrite Material |
| title_short | <i>Thermithiobacillus plumbiphilus</i> AAFK—Arsenic-Resistant Bacteria Isolated from Arsenopyrite Material |
| title_sort | i thermithiobacillus plumbiphilus i aafk arsenic resistant bacteria isolated from arsenopyrite material |
| topic | <i>Thermithiobacillus plumbiphilus</i> arsenopyrite arsenic resistance sulfur compounds oxidation |
| url | https://www.mdpi.com/2036-7481/16/1/14 |
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