Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigs
Abstract Sepsis is a severe disease and induces skeletal muscle atrophy, which has a significant impact on patients. Moreover, the onset and severity of muscle atrophy can vary across different anatomical regions during sepsis. Our previous study demonstrated that ZBED6 knockout not only promotes sk...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05620-6 |
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| author | Huan Liu Wen Shi Yanjun Zheng Song Yang Yihui Wang Maosen Yang Zhitao Yang Enqiang Mao Hanbing Shang Erzhen Chen Ying Chen |
| author_facet | Huan Liu Wen Shi Yanjun Zheng Song Yang Yihui Wang Maosen Yang Zhitao Yang Enqiang Mao Hanbing Shang Erzhen Chen Ying Chen |
| author_sort | Huan Liu |
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| description | Abstract Sepsis is a severe disease and induces skeletal muscle atrophy, which has a significant impact on patients. Moreover, the onset and severity of muscle atrophy can vary across different anatomical regions during sepsis. Our previous study demonstrated that ZBED6 knockout not only promotes skeletal muscle growth under physiological conditions, but also alleviates systemic muscle atrophy during sepsis. However, its region-specific effects on sepsis-induced muscle atrophy have not been analyzed. In this study, we performed RNA sequencing for 54 samples of skeletal muscle from nine different anatomical regions from male ZBED6 knockout and wild-type (WT) minipigs subjected to sepsis. We generated 364.63 Gb of high-quality bulk RNA sequencing data from the skeletal muscle samples (approximately 6.75 Gb per sample). This dataset provided insightful gene expression information to understand the role of ZBED6 in region-specific muscle atrophy during sepsis. In addition, this dataset can be used for heterogeneity analysis between skeletal muscle tissues from multiple species or female pigs. |
| format | Article |
| id | doaj-art-6b2b2ba91f7c45f5ae6eef9168f5aaf7 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-6b2b2ba91f7c45f5ae6eef9168f5aaf72025-08-20T03:42:23ZengNature PortfolioScientific Data2052-44632025-07-011211710.1038/s41597-025-05620-6Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigsHuan Liu0Wen Shi1Yanjun Zheng2Song Yang3Yihui Wang4Maosen Yang5Zhitao Yang6Enqiang Mao7Hanbing Shang8Erzhen Chen9Ying Chen10Department of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi UniversityDepartment of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineShanghai Institute of Aviation Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineAbstract Sepsis is a severe disease and induces skeletal muscle atrophy, which has a significant impact on patients. Moreover, the onset and severity of muscle atrophy can vary across different anatomical regions during sepsis. Our previous study demonstrated that ZBED6 knockout not only promotes skeletal muscle growth under physiological conditions, but also alleviates systemic muscle atrophy during sepsis. However, its region-specific effects on sepsis-induced muscle atrophy have not been analyzed. In this study, we performed RNA sequencing for 54 samples of skeletal muscle from nine different anatomical regions from male ZBED6 knockout and wild-type (WT) minipigs subjected to sepsis. We generated 364.63 Gb of high-quality bulk RNA sequencing data from the skeletal muscle samples (approximately 6.75 Gb per sample). This dataset provided insightful gene expression information to understand the role of ZBED6 in region-specific muscle atrophy during sepsis. In addition, this dataset can be used for heterogeneity analysis between skeletal muscle tissues from multiple species or female pigs.https://doi.org/10.1038/s41597-025-05620-6 |
| spellingShingle | Huan Liu Wen Shi Yanjun Zheng Song Yang Yihui Wang Maosen Yang Zhitao Yang Enqiang Mao Hanbing Shang Erzhen Chen Ying Chen Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigs Scientific Data |
| title | Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigs |
| title_full | Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigs |
| title_fullStr | Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigs |
| title_full_unstemmed | Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigs |
| title_short | Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigs |
| title_sort | bulk rna seq of skeletal muscle reveals the role of zbed6 in sepsis induced muscle atrophy in minipigs |
| url | https://doi.org/10.1038/s41597-025-05620-6 |
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