Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors
<b>Aim:</b> The study objectives were to determine whether dietary supplementation with long chain omega-3 polyunsaturated fatty acids in the form of enriched fish oil (FO) for 90 days or treatment with bovine somatotropin (bST) at the time of ovulation and 11 days post-ovulation influen...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=101 |
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| author | Leslie A. MacLaren Todd R. Bilby Frank Michel Aydin Guzeloglu Charles R. Staples William W. Thatcher |
| author_facet | Leslie A. MacLaren Todd R. Bilby Frank Michel Aydin Guzeloglu Charles R. Staples William W. Thatcher |
| author_sort | Leslie A. MacLaren |
| collection | DOAJ |
| description | <b>Aim:</b> The study objectives were to determine whether dietary
supplementation with long chain omega-3 polyunsaturated
fatty acids in the form of enriched fish oil (FO)
for 90 days or treatment with bovine somatotropin (bST) at
the time of ovulation and 11 days post-ovulation influenced
PPAR expression and activation in bovine endometrium in
Holstein cows.<p>
<b>Materials and Methods:</b> Non-lactating cows were assigned
to one of four treatments: cyclic, cyclic-bST, pregnant
or pregnant-bST. Lactating cows were assigned to one of
six treatments: cyclic, cyclic-bST, pregnant, pregnant-bST,
cyclic-FO or cyclic-FO-bST.<p>
<b>Results:</b> Northern and Western blot analyses indicated
that PPARα and PPARδ, but not PPARγ, are expressed in
endometrium from all cows at day 17 post-ovulation. Treatment
with bST is associated with increased PPARδ mRNA
abundance in pregnant but not cyclic cows, suggesting that
the effect may be mediated by the embryo. Increased abundances
of PPARα mRNA are observed in response to bST
during pregnancy in lactating cows but not in non-lactating
cows, highlighting the importance of lactation status in determining
bST response. Fish oil supplementation is associated
with reduced PPARδ mRNA abundance, but did not affect
steady-state PPARα mRNA abundance. PPARδ protein is
expressed in the luminal epithelium, glandular epithelium,
subepithelial stroma and to a lesser extent in the adluminal
stroma. Anti-PPARδ reactivity is reduced in response to bST
and fish oil treatments in pregnant cows.<p>
<b>Conclusion:</b> bST and fish oil treatments affect endometrial
PPARα and PPARδ expression in lactating dairy cows. |
| format | Article |
| id | doaj-art-6b146f6dc7234f56b7f6e1a98c993654 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-6b146f6dc7234f56b7f6e1a98c9936542025-02-03T11:25:41ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-1953274207218101Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptorsLeslie A. MacLarenTodd R. Bilby0Frank Michel1Aydin Guzeloglu2Charles R. Staples3William W. Thatcher4Department of Animal Sciences, University of Florida, Gainesville, Florida, USADepartment of Animal Sciences, University of Florida, Gainesville, Florida, USADepartment of Genetic, Faculty of Veterinary Medicine, Selcuk University, Konya, TurkeyDepartment of Animal Sciences, University of Florida, Gainesville, Florida, USADepartment of Animal Sciences, University of Florida, Gainesville, Florida, USA<b>Aim:</b> The study objectives were to determine whether dietary supplementation with long chain omega-3 polyunsaturated fatty acids in the form of enriched fish oil (FO) for 90 days or treatment with bovine somatotropin (bST) at the time of ovulation and 11 days post-ovulation influenced PPAR expression and activation in bovine endometrium in Holstein cows.<p> <b>Materials and Methods:</b> Non-lactating cows were assigned to one of four treatments: cyclic, cyclic-bST, pregnant or pregnant-bST. Lactating cows were assigned to one of six treatments: cyclic, cyclic-bST, pregnant, pregnant-bST, cyclic-FO or cyclic-FO-bST.<p> <b>Results:</b> Northern and Western blot analyses indicated that PPARα and PPARδ, but not PPARγ, are expressed in endometrium from all cows at day 17 post-ovulation. Treatment with bST is associated with increased PPARδ mRNA abundance in pregnant but not cyclic cows, suggesting that the effect may be mediated by the embryo. Increased abundances of PPARα mRNA are observed in response to bST during pregnancy in lactating cows but not in non-lactating cows, highlighting the importance of lactation status in determining bST response. Fish oil supplementation is associated with reduced PPARδ mRNA abundance, but did not affect steady-state PPARα mRNA abundance. PPARδ protein is expressed in the luminal epithelium, glandular epithelium, subepithelial stroma and to a lesser extent in the adluminal stroma. Anti-PPARδ reactivity is reduced in response to bST and fish oil treatments in pregnant cows.<p> <b>Conclusion:</b> bST and fish oil treatments affect endometrial PPARα and PPARδ expression in lactating dairy cows.http://eurasianjvetsci.org/pdf.php3?id=101cowendometriumpparbstfish oil |
| spellingShingle | Leslie A. MacLaren Todd R. Bilby Frank Michel Aydin Guzeloglu Charles R. Staples William W. Thatcher Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors Eurasian Journal of Veterinary Sciences cow endometrium ppar bst fish oil |
| title | Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors |
| title_full | Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors |
| title_fullStr | Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors |
| title_full_unstemmed | Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors |
| title_short | Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors |
| title_sort | supplementation of dairy cows with bovine somatotropin or omega 3 rich fish oil affects the endometrial expression of peroxisome proliferator activated receptors |
| topic | cow endometrium ppar bst fish oil |
| url | http://eurasianjvetsci.org/pdf.php3?id=101 |
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