DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status
Intervertebral disc (IVD) degeneration is the primary cause for low back pain that has a high prevalence in modern society and poses enormous economic burden on patients. Few effective therapeutic strategies are available for IVD degeneration treatment. To understand the biological effects of dental...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/7590337 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548821517729792 |
|---|---|
| author | Xiwen Dong Fanqi Hu Jing Yi Yuning Zhang Chao Liu Panpan Geng Han Duan Chu-Tse Wu Xuesong Zhang Hua Wang |
| author_facet | Xiwen Dong Fanqi Hu Jing Yi Yuning Zhang Chao Liu Panpan Geng Han Duan Chu-Tse Wu Xuesong Zhang Hua Wang |
| author_sort | Xiwen Dong |
| collection | DOAJ |
| description | Intervertebral disc (IVD) degeneration is the primary cause for low back pain that has a high prevalence in modern society and poses enormous economic burden on patients. Few effective therapeutic strategies are available for IVD degeneration treatment. To understand the biological effects of dental pulp stem cells (DPSCs) on nucleus pulposus (NP) cells, we carried out RNA sequencing, bioinformatic analysis which unveiled gene expression differences, and pathway variation in primarily isolated patients’ NP cells after treatment with DPSCs supernatant. Western blot and immunofluorescence were used to verify these molecular alterations. Besides, to evaluate the therapeutic effect of DPSCs in IVD degeneration treatment, DPSCs were injected into a degeneration rat model in situ, with treatment outcome measured by micro-CT and histological analysis. RNA sequencing and in vitro experiments demonstrated that DPSCs supernatant could downregulate NP cells’ inflammation-related NF-κB and JAK-STAT pathways, reduce IL-6 production, increase collagen II expression, and mitigate apoptosis. In vivo results showed that DPSCs treatment protected the integrity of the disc structure, alleviated extracellular matrix degradation, and increased collagen fiber expression. In this study, we verified the therapeutic effect of DPSCs in an IVD degeneration rat model and elucidated the underlying molecular mechanism of DPSCs treatment, which provides a foundation for the application of DPSCs in IVD degeneration treatment. |
| format | Article |
| id | doaj-art-6afdb79c7dfb4a24ac00f4e8b96413c9 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6afdb79c7dfb4a24ac00f4e8b96413c92025-02-03T06:13:01ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/7590337DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune StatusXiwen Dong0Fanqi Hu1Jing Yi2Yuning Zhang3Chao Liu4Panpan Geng5Han Duan6Chu-Tse Wu7Xuesong Zhang8Hua Wang9Department of Experimental HematologyMedical School of Chinese PLADepartment of Experimental HematologyDepartment of Experimental HematologyDepartment of OrthopaedicsDepartment of Experimental HematologyDepartment of Experimental HematologyDepartment of Experimental HematologyDepartment of OrthopaedicsDepartment of Experimental HematologyIntervertebral disc (IVD) degeneration is the primary cause for low back pain that has a high prevalence in modern society and poses enormous economic burden on patients. Few effective therapeutic strategies are available for IVD degeneration treatment. To understand the biological effects of dental pulp stem cells (DPSCs) on nucleus pulposus (NP) cells, we carried out RNA sequencing, bioinformatic analysis which unveiled gene expression differences, and pathway variation in primarily isolated patients’ NP cells after treatment with DPSCs supernatant. Western blot and immunofluorescence were used to verify these molecular alterations. Besides, to evaluate the therapeutic effect of DPSCs in IVD degeneration treatment, DPSCs were injected into a degeneration rat model in situ, with treatment outcome measured by micro-CT and histological analysis. RNA sequencing and in vitro experiments demonstrated that DPSCs supernatant could downregulate NP cells’ inflammation-related NF-κB and JAK-STAT pathways, reduce IL-6 production, increase collagen II expression, and mitigate apoptosis. In vivo results showed that DPSCs treatment protected the integrity of the disc structure, alleviated extracellular matrix degradation, and increased collagen fiber expression. In this study, we verified the therapeutic effect of DPSCs in an IVD degeneration rat model and elucidated the underlying molecular mechanism of DPSCs treatment, which provides a foundation for the application of DPSCs in IVD degeneration treatment.http://dx.doi.org/10.1155/2022/7590337 |
| spellingShingle | Xiwen Dong Fanqi Hu Jing Yi Yuning Zhang Chao Liu Panpan Geng Han Duan Chu-Tse Wu Xuesong Zhang Hua Wang DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status Stem Cells International |
| title | DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status |
| title_full | DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status |
| title_fullStr | DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status |
| title_full_unstemmed | DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status |
| title_short | DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status |
| title_sort | dpscs protect architectural integrity and alleviate intervertebral disc degeneration by regulating nucleus pulposus immune status |
| url | http://dx.doi.org/10.1155/2022/7590337 |
| work_keys_str_mv | AT xiwendong dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT fanqihu dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT jingyi dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT yuningzhang dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT chaoliu dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT panpangeng dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT handuan dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT chutsewu dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT xuesongzhang dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus AT huawang dpscsprotectarchitecturalintegrityandalleviateintervertebraldiscdegenerationbyregulatingnucleuspulposusimmunestatus |